口腔医学研究

口腔医学研究 ›› 2023, Vol. 39 ›› Issue (5): 422-428.DOI: 10.13701/j.cnki.kqyxyj.2023.05.009

• 口腔材料学研究 • 上一篇    下一篇

三氯生提高树脂水门汀抗菌性能的研究

侯妍妍, 陈欢, 冯杉杉, 丁景瑜, 朱松*   

  1. 吉林大学口腔医院修复科 吉林 长春 130021
  • 收稿日期:2022-09-27 出版日期:2023-05-28 发布日期:2023-05-16
  • 通讯作者: *朱松,E-mail: zhusong1965@163.com
  • 作者简介:侯妍妍(1996~ ),女,吉林长春人,硕士在读,研究方向:口腔材料学。
  • 基金资助:
    吉林省科技发展计划项目(编号:YDZJ202202CXJD044)

Study on Triclosan in Improving the Antibacterial Properties of Resin Cement

HOU Yanyan, CHEN Huan, FENG Shanshan, DING Jingyu, ZHU Song*   

  1. Department of Prosthodontics, Stomatology Hospital, Jilin University, Changchun 130021, China
  • Received:2022-09-27 Online:2023-05-28 Published:2023-05-16

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摘要： 目的: 制备一种含可光固化三氯生的抗菌树脂水门汀,并对其表征性能、力学性能、粘接强度、抗菌性能及体外细胞毒性进行评估。方法: 将可光固化三氯生衍生物(TCS-IH)以25wt%溶于丙酮后,按照混合后0、2、4、6、8 wt%的质量比加入商品树脂水门汀Multilink Speed中。采用FTIR检测各组树脂水门汀固化前后的双键转化率;静态水接触角法检测各组树脂水门汀的表面润湿性;用三点弯曲实验测试弯曲强度;微拉伸实验检测即刻粘接性能;选择变异链球菌进行抗菌实验,通过菌落计数法、MTT实验、活/死细菌荧光染色评价树脂水门汀的抗菌性能;并通过CCK-8进行细胞毒性的测定,评价体外细胞毒性等级。结果: 2 wt%和4 wt%组对树脂水门汀的表征性能、力学性能及粘接强度无明显影响(P>0.05 );当TCS-IH含量为4 wt%、6 wt%、8 wt%时,树脂水门汀均具有显著的抗菌效果(P<0.05 );2 wt%和4 wt%组树脂水门汀均具有良好的生物相容性。结论: 当TCS-IH的质量百分比为4 wt%时,在有效提高树脂水门汀抗菌性能的同时,并未影响其表征性能、力学性能、粘接性能及生物安全性。

关键词: 树脂水门汀, 自粘接, 可光固化三氯生, 抗菌性能, 粘接性能

Abstract: Objective: To prepare antibacterial resin cement containing photocurable triclosan derivative, and to evaluate its characterization performance, mechanical properties, adhesive strength, antibacterial properties, and cytotoxicity in vitro. Methods: The photocurable triclosan derivative (TCS-IH) was dissolved in acetone at 25 wt% and then added to Multilink Speed at a mass ratio of 0 wt%, 2 wt%, 4 wt%, 6 wt%, and 8 wt%, to obtain the resin cement with antibacterial properties for different components. FTIR was used to examine the conversion of double bonds before and after curing of resin cement in each group. The surface wettability of resin cement in each group was examined by static water contact angle. Its flexure strength was tested with a three-point bending test. Micro-tensile test was used to examine its instant bonding properties. S.mutans was selected for antibacterial experiments, and the antibacterial properties of resin cements were evaluated by colony counting, MTT, and live/dead bacterial fluorescence staining. The cytotoxicity grade in vitro was evaluated by CCK-8 cytotoxicity assay. Results: The addition of TCS-IH acetone solution at 2 wt% and 4 wt% had no significant effect on characterization performance, mechanical properties, and bonding strength of resin cement (P>0.05). When the mass percentage of TCS-IH reached 4 wt%, 6 wt%, and 8 wt%, resin cement all showed significant antimicrobial activity (P<0.05). Both 2 wt% and 4 wt% resin cements had good biocompatibility. Conclusion: When the mass percentage of TCS-IH was 4 wt%, the antibacterial properties of resin cement were effectively improved while its characterization properties, mechanical properties, adhesive properties, and biosafety were not affected.

Key words: resin cement, self bonding, photocurable triclosan derivative, antibacterial properties, adhesion property