口腔医学研究

口腔医学研究 ›› 2023, Vol. 39 ›› Issue (10): 911-916.DOI: 10.13701/j.cnki.kqyxyj.2023.10.012

• 口腔颌面外科学研究 • 上一篇    下一篇

辣椒素纳米粒的制作及其在治疗大鼠三叉神经痛中的作用

熊雨1,2,3, 郑欣欣1,2,3, 陈敬林1,2,3, 陈丽珍1,2,3, 金幼虹1,2,3*, 王沛2,3   

  1. 1.南昌大学附属口腔医院牙周科 江西 南昌 330006;
    2.江西省口腔生物医学重点实验室 江西 南昌 330006;
    3.江西省口腔疾病临床医学研究中心 江西 南昌 330006
  • 收稿日期:2023-04-17 出版日期:2023-10-28 发布日期:2023-10-25
  • 通讯作者: *金幼虹,E-mail:2670954014@qq.com
  • 作者简介:熊雨(1996~ ),女,江西丰城人,硕士,研究方向:三叉神经痛。
  • 基金资助:
    江西省科技厅重点研发计划项目(编号:20192BBG70020),江西省卫健委科技计划课题(编号:SKJP 220202269)

Preparation of Capsaicin Nanoparticles and Their Effect on Treatment of Trigeminal Neuralgia in Rats

XIONG Yu1,2,3, ZHENG Xinxin1,2,3, CHEN Jinglin1,2,3, CHEN Lizhen1,2,3, JIN Youhong1,2,3*, WANG Pei2,3   

  1. 1. Department of Periodontology, The Affiliated Stomatological Hospital of Nanchang University, Nanchang 330006, China;
    2. The Key Laboratory of Oral Biomedicine, Nanchang 330006, China;
    3. Jiangxi Province Clinical Research Center for Oral Diseases, Nanchang 330006, China
  • Received:2023-04-17 Online:2023-10-28 Published:2023-10-25

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摘要： 目的: 将辣椒素(capsaicin,Cap)制备成纳米颗粒,并进一步制成凝胶观察其对大鼠三叉神经痛的治疗作用。方法: 以聚乳酸-羟基乙酸(polylactic-co-glycolic acid,PLGA)为载体,以牛血清白蛋白(bovine serum albumin, BSA)为稳定剂,负载辣椒素,采用乳液溶剂蒸发法制备辣椒素纳米粒(polylactic-co-glycolic acid/capsaicin nanoparticles,PLGA/Cap NPs)。利用扫描电子显微镜-能量色散光谱(scanning electron microscope,SEM)、傅立叶变换红外光谱(fourier transform infrared spectroscopy,FTIR)、高灵敏度Zeta电位和纳米颗粒尺寸分析仪对纳米颗粒进行了表征,用差示扫描量热法(differential scanning caborimetry,DSC)分析了纳米颗粒的性质。将辣椒素纳米颗粒用卡波姆934制备成凝胶,采用高效液相色谱法(high performance liquid chromatography,HPLC)测定载药量、包封率及体外释药性能,将其应用于大鼠三叉神经痛手术部位,观察其疗效。结果: 17 mg剂量的PLGA/Cap NPs呈规则球形,水化粒径为(389.58±12.02) nm, 聚合物分散性指数(polymer dispersity index,PDI)为0.270±0.018,Zeta电位为(33.56±2.61) mV,载药量为(57.56±0.05)%,包封率为(90.26±0.90)%,最大累积释放率为77.19%。在大鼠手术部位涂抹0.03%、0.3%和3% PLGA/Cap NPs凝胶后,疼痛阈值明显升高(P＜0.05)。敷药组P物质(substance P,SP)和前列腺素E2(prostaglandin E2,PGE2)血清中的浓度较模型组显著下降。结论: 辣椒素纳米凝胶对大鼠三叉神经痛有一定的治疗作用。

关键词: 辣椒素, 三叉神经痛, P物质, 前列腺素E2

Abstract: Objective: To prepare capsaicin (Cap) nanoparticles and develop them into gel to observe their therapeutic effect on trigeminal neuralgia in rats. Methods: Cap-loaded polylactic-co-glycolic acid/Cap nanoparticles (PLGA/Cap NPs) were prepared using PLGA as the carrier and bovine serum albumin as the stabilizer by emulsion solvent evaporation method. The nanoparticles were characterized by scanning electron microscopy-energy dispersive spectroscopy, Fourier transform infrared spectroscopy, high sensitivity zeta potential analyzer, and nanoparticle size analyzer. Differential scanning calorimetry was used to analyze the properties of the nanoparticles. The Cap nanoparticles were then prepared into gel with Carbopol 934. The drug loading capacity, encapsulation efficiency, and in vitro release performance were determined by high-performance liquid chromatography. Finally, they were applied to the surgical site of rat trigeminal neuralgia to observe the efficacy. Results: At a dose of 17 mg, PLGA/Cap NPs had regular spherical shape with hydrated particle size of (389.58±12.02) nm; polymer dispersity index (PDI) of 0.270±0.018; Zeta potential of (33.56±2.61) mV; drug loading capacity of (57.56±0.05)%; encapsulation efficiency of (90.26±0.90)%; and maximum cumulative release rate of 77.19%. After applying 0.03%, 0.3%, or 3% PLGA/Cap NP gels to the surgical site in rats' faces, trigeminal pain threshold was significantly increased (P<0.05). The level of substance P (SP) and prostaglandin E2 (PGE2) in serum were significantly decreased in the treatment group compared with the model group (P<0.05). Conclusion: Cap nano-gel has certain therapeutic effects on rat trigeminal neuralgia.

Key words: capsaicin, trigeminal neuralgia, substance P, prostaglandin E2