口腔医学研究

口腔医学研究 ›› 2023, Vol. 39 ›› Issue (12): 1097-1105.DOI: 10.13701/j.cnki.kqyxyj.2023.12.013

• 口腔生物学研究 • 上一篇    下一篇

镁离子影响人上颌窦黏膜干细胞体外成骨分化的研究

张致浩1,2, 张秀秀3, 赵思楠1,2, 宫宁1,2, 王英1, 谈飞1,2*   

  1. 1.青岛大学附属医院口腔修复科 山东 青岛 266003;
    2.青岛大学 口腔医学院 山东 青岛 266023;
    3.青岛大学附属青岛市第三人民医院口腔科 山东 青岛 266041
  • 收稿日期:2023-06-10 出版日期:2023-12-28 发布日期:2023-12-25
  • 通讯作者: * 谈飞,E-mail:tanf_1984@163.com
  • 作者简介:张致浩(1998~ ),男,济南人,硕士在读,主要从事口腔修复临床及基础研究。
  • 基金资助:
    山东省自然科学基金面上项目(编号:ZR2022MH239)

Effect of Magnesium Ions on Biological Activity of Human Maxillary Sinus Membrane Stem Cells

ZHANG Zhihao1,2, ZHANG Xiuxiu3, ZHAO Sinan1,2, GONG Ning1,2, WANG Ying1, TAN Fei1,2   

  1. 1. Department of Prosthodontics, The Affiliated Hospital of Qingdao University, Qingdao 266003, China;
    2. School of Stomatology, Qingdao University, Qingdao 266023, China;
    3. Department of Stomatology, Affiliated Qingdao Third People's Hospital, Qingdao University, Qingdao 266041, China
  • Received:2023-06-10 Online:2023-12-28 Published:2023-12-25

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摘要： 目的:研究不同浓度镁离子(Mg2+)对人上颌窦黏膜干细胞(human maxillary sinus membrane stem cells,hMSMSCs)生物行为的影响。方法:体外提取hMSMSCs后利用流式细胞术和多向分化实验来鉴定其间充质干细胞特征,随后将细胞分别培养在0.8 mmol/L、1.8 mmol/L、2.8 mmol/L和5.8 mmol/L的Mg2+浓度下。通过CCK-8实验和Calcein AM/PI染色检测hMSMSCs的细胞黏附和增殖,采用罗丹明鬼笔环肽-DAPI染色评估细胞的黏附伸展。对成骨诱导培养后的hMSMSCs进行ALP活性、ALP染色和茜素红染色,同时利用qRT-PCR实验检测成骨相关生长因子的表达差异。结果:适宜浓度Mg2+能有效改善hMSMSCs的黏附、伸展和增殖。1.8 mmol/L Mg2+提高了hMSMSCs的ALP活性,并显著改善细胞外钙结节的形成并上调成骨相关基因的表达,而过高浓度的Mg2+相对抑制了hMSMSCs的成骨分化(P＜0.05)。结论:适宜浓度的Mg2+(1.8 mmol/L)可以促进hMSMSCs的细胞骨架的伸展和黏附,并有利于增殖和成骨分化。

关键词: 间充质干细胞, 成骨, 上颌窦, 镁离子

Abstract: Objective: To investigate the effects of different concentrations of magnesium ions (Mg2+) on the biological behavior of human maxillary sinus membrane stem cells (hMSMSCs). Methods: hMSMSCs were extracted in vitro, and flow cytometry and multilineage differentiation experiments were conducted to identify their mesenchymal stem cell characteristics. Subsequently, the cells were cultured at concentrations of 0.8 mmol/L, 1.8 mmol/L, 2.8 mmol/L, and 5.8 mmol/L of Mg2+. Cell adhesion and proliferation of hMSMSCs were evaluated using CCK-8 assay and Calcein AM/PI staining, while cell adhesion extension was assessed using Rhodamine phalloidin-DAPI staining. ALP activity, ALP staining, and Alizarin Red staining were performed on hMSMSCs after osteogenic induction culture, and qRT-PCR experiments were conducted to detect the expression differences of osteogenic-related growth factors. Results: Optimal concentration of Mg2+ effectively improved cell adhesion, extension, and proliferation of hMSMSCs. 1.8 mmol/L Mg2+ enhanced ALP activity in hMSMSCs, significantly improved extracellular calcium nodule formation, and upregulated the expression of osteogenic-related genes. Conversely, excessively high Mg2+ concentrations inhibited osteogenic differentiation of hMSMSCs (P＜0.05). Conclusion: Appropriate concentration of Mg2+ (1.8 mmol/L) promotes cell spreading and adhesion of hMSMSCs, facilitating proliferation and osteogenic differentiation.

Key words: mesenchymal stem cell, osteogenesis, maxillary sinus, magnesium ion