口腔医学研究

口腔医学研究 ›› 2025, Vol. 41 ›› Issue (12): 1065-1069.DOI: 10.13701/j.cnki.kqyxyj.2025.12.009

• 口腔颌面外科学研究 • 上一篇    下一篇

碘基增强Micro-CT成像技术辅助病理评估口腔鳞状细胞癌区域淋巴结状态的可行性研究

顾洲宇, 贾辉, 王育新*, 夏成万*   

  1. 南京大学医学院附属口腔医院,南京市口腔医院口腔颌面创伤正颌整形外科,南京大学口腔医学研究所 江苏 南京 210008
  • 收稿日期:2025-04-17 出版日期:2025-12-28 发布日期:2025-12-23
  • 通讯作者: *王育新,E-mail:wangyuxin0212@126.com; 夏成万,E-mail:2665927917@qq.com
  • 作者简介:顾洲宇(1998~ ),男,江苏人,硕士在读,住院医师,研究方向:口腔颌面外科学。
  • 基金资助:
    国自然青年科学基金(编号:82403466)南京市卫生科技发展项目(编号:YKK22177)南京市口腔医院高水平医院建设科研项目(编号:0224c028)

Feasibility Study on Lugol's Iodine Enhanced Micro-CT Imaging to Assist Pathological Assessment of Lymph Node Status of Oral Squamous Cell Carcinoma

GU Zhouyu, JIA Hui, WANG Yuxin*, XIA Chengwan*   

  1. Department of Oral and Maxillofacial Trauma Orthognathic Plastic Surgery, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Research Institute of Stomatology, Nanjing University, Nanjing 210008, China
  • Received:2025-04-17 Online:2025-12-28 Published:2025-12-23

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摘要： 目的: 准确评估区域淋巴结转移状态对于口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)诊治至关重要,本研究拟利用碘基增强Micro-CT成像技术辅助病理切片三维评估OSCC区域淋巴结状态。方法: 收集75枚OSCC患者离体淋巴结样本,经3% Lugol’s iodine染色12 h后行Micro-CT扫描。随后将样本行常规苏木精-伊红(hematoxylin-eosin, HE)染色切片,制作数字化病理切片。随后对比Micro-CT图像与病理图像对淋巴结转移状态的评估情况。结果: 碘基增强Micro-CT成像可以清晰勾勒淋巴结的转移灶,以二维病理切片为金标准,其特异度为90.4％,灵敏度为91.3％,准确度为90.7％。结论: 碘基增强Micro-CT成像可以三维准确评估OSCC的离体淋巴结转移状态,有效降低单纯二维病理切片对微小淋巴结转移的漏诊,为OSCC术后辅助治疗的制定提供更准确的依据。

关键词: 口腔鳞状细胞癌, 淋巴结, 卢戈氏碘液, 显微计算机断层成像

Abstract: Objective: To evaluate the status of regional lymph nodes in oral squamous cell carcinoma (OSCC) with Lugol's iodine enhanced micro-computed tomography (Micro-CT) imaging. Methods: Seventy-five isolated lymph node samples were collected from OSCC patients, and were stained with 3% Lugol's iodine for 12 hours before Micro-CT scan. The samples were sliced by conventional hematoxylin-eosin (H&E) staining, and digital pathological sections were made. The evaluation of lymph node metastasis status by Micro-CT image and pathological image was compared. Results: Lugol's iodine enhanced Micro-CT imaging could delineate lymph node metastasis clearly. 2D pathological sections were used as gold standard, with specificity of 90.4%, sensitivity of 91.3%, and accuracy of 90.7%. Conclusion: Lugol's iodine enhanced Micro-CT imaging can accurately evaluate the status of isolated lymph node metastasis in OSCC in three dimensions, effectively reduce the missed diagnosis of microlymph node metastasis in simple two-dimensional pathological sections, and provide a more accurate basis for the formulation of postoperative adjuvant treatment for OSCC.

Key words: OSCC, lymph node, Lugol’s iodine, Micro-CT