COL7A1和LAMC2在口腔鳞状细胞癌中的表达及预后意义

doi:10.13701/j.cnki.kqyxyj.2024.07.012

口腔医学研究 ›› 2024, Vol. 40 ›› Issue (7): 634-639.DOI: 10.13701/j.cnki.kqyxyj.2024.07.012

COL7A1和LAMC2在口腔鳞状细胞癌中的表达及预后意义

刘家琦¹, 梁登桓¹, 张佳佳¹, 郭超杰¹, 刘昱词¹, 徐江¹, 曾妍², 郑军^1*

1.石河子大学第一附属医院口腔科新疆石河子 832000;
2.广东医科大学湛江中心医院精准医学检验实验室广东湛江 524000

收稿日期:2024-03-26 出版日期:2024-07-28 发布日期:2024-07-24
通讯作者: *郑军,E-mail: haohaoay@yeah.net
作者简介:刘家琦(1996~ ),男,河南许昌人,硕士在读,主要从事口腔医学相关研究工作。
基金资助:
国家自然科学基金(编号:81560473);广东省自然科学基金面上项目(编号:2024A1515010858)

Expression and Prognostic Significance of COL7A1 and LAMC2 in Oral Squamous Cell Carcinoma.

LIU Jiaqi¹, LIANG Denghuan¹, ZHANG Jiajia¹, GUO Chaojie¹, LIU Yuci¹, XU Jiang¹, ZENG Yan², ZHENG Jun^1*

1. Department of Stomatology, The First Affiliated Hospital of Shihezi University, Shihezi 832002, China;
2. Laboratory of Precision Medicine, Zhanjiang Central Hospital of Guangdong Medical University, Zhanjiang 524000, China

Received:2024-03-26 Online:2024-07-28 Published:2024-07-24

摘要/Abstract

摘要： 目的:探讨口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)中Ⅶ胶原蛋白 α-1链(type Ⅶ Collagen α-1 chain,COL7A1)和层粘连蛋白γ-2(laminin γ-2,LAMC2)的表达水平,明确COL7A1和LAMC2的表达水平与OSCC患者临床病理特征及预后的相关性。方法:应用免疫组织化学法检测108例OSCC组织和55例癌旁组织中COL7A1和LAMC2的表达,分析COL7A1和LAMC2表达水平与OSCC患者临床病理特征及预后的关系;采用实时荧光定量聚合酶链反应和Western blot检测OSCC细胞系中COL7A1和LAMC2的表达量;应用生物信息学数据库进行验证。结果:OSCC组织中COL7A1和LAMC2的表达均显著高于癌旁组织(P<0.001),且两者的表达呈正相关(r=0.655,P<0.001);COL7A1的表达与T分期、TNM分期及淋巴结转移有关(P<0.05),LAMC2的表达与TNM分期和淋巴结转移有关(P<0.05);与正常口腔角质细胞相比,COL7A1和LAMC2在OSCC 细胞系中均呈过表达(P<0.05);生存分析显示,COL7A1和LAMC2高表达组的总生存期均短于低表达组(P<0.05);多因素Cox回归分析表明COL7A1和LAMC2是影响OSCC患者预后的独立危险因素(P<0.05)。结论:COL7A1和LAMC2在OSCC中表达增高,与肿瘤的TNM分期以及转移有关,对OSCC患者的预后评估具有一定的参考价值。

关键词: 口腔鳞状细胞癌, Ⅶ型胶原蛋白, 层粘连蛋白γ2, 预后

Abstract: Objective: To investigate the expression of type Ⅶ Collagen α-1 chain (COL7A1) and laminin γ-2 (LAMC2) in oral squamous cell carcinoma, and to clarify the correlation between the expression of COL7A1 and LAMC2 and clinical parameters and prognosis in OSCC patients. Methods: Immunohistochemistry was used to detect the expression of COL7A1 and LAMC2 in 108 cases of OSCC tissues and 55 cases of adjacent tissues, and to analyze the correlation between COL7A1 and LAMC2 and the clinicopathological features and prognosis of OSCC patients. The expression levels of COL7A1 and LAMC2 in OSCC cell lines were detected by real-time fluorescence quantitative PCR and western blotting. Finally, the bioinformatics database was used for verification. Results: The expressions of COL7A1 and LAMC2 in OSCC tissues were significantly higher than those in adjacent tissues (P<0.001), and the expressions of COL7A1 and LAMC2 were positively correlated (r=0.655,P<0.001). The expression of COL7A1 was related to T stage, TNM stage, and lymph node metastasis (P<0.05), and that of LAMC2 was related to TNM stage and lymph node metastasis (P<0.05). Compared with normal oral keratinocytes, COL7A1 and LAMC2 were overexpressed in OSCC cell lines (P<0.05). Kaplan-Meier analysis showed that the overall survival time of the high expression group of COL7A1 and LAMC2 was shorter than that of the low expression group (P<0.05). Multiple Cox regression analysis showed that COL7A1 and LAMC2 was an independent risk factor for OSCC patients (P<0.05). Conclusion: The increased expression of COL7A1 and LAMC2 in OSCC has certain reference value for the prognosis evaluation of patients with oral squamous cell carcinoma, and is related to the TNM stage and metastasis of the tumor.

Key words: oral squamous cell carcinoma, type Ⅶ collagen, laminin γ2, prognosis

刘家琦, 梁登桓, 张佳佳, 郭超杰, 刘昱词, 徐江, 曾妍, 郑军. COL7A1和LAMC2在口腔鳞状细胞癌中的表达及预后意义[J]. 口腔医学研究, 2024, 40(7): 634-639.

LIU Jiaqi, LIANG Denghuan, ZHANG Jiajia, GUO Chaojie, LIU Yuci, XU Jiang, ZENG Yan, ZHENG Jun. Expression and Prognostic Significance of COL7A1 and LAMC2 in Oral Squamous Cell Carcinoma.[J]. Journal of Oral Science Research, 2024, 40(7): 634-639.

参考文献

[1] Tan Y, Wang Z, Xu M, et al. Oral squamous cell carcinomas: state of the field and emerging directions [J]. Int J Oral Sci, 2023, 15(1):44.
[2] Sung H, Ferlay J, Siegel RL, et al. Global Cancer Statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries [J]. CA Cancer J Clin, 2021, 71(3):209-249.
[3] Badwelan M, Muaddi H, Ahmed A, et al.Oral squamous cell carcinoma and concomitant primary tumors, What do we know? A review of the literature [J]. Curr Oncol, 2023, 30(4): 3721-3734.
[4] Pekarek L, Garrido-Gil MJ, Sánchez-Cendra A, et al. Emerging histological and serological biomarkers in oral squamous cell carcinoma: Applications in diagnosis, prognosis evaluation and personalized therapeutics (Review) [J]. Oncol Rep, 2023, 50(6):213.
[5] Tartaglia G, Cao Q, Padron ZM, et al. Impaired wound healing, fibrosis, and cancer: The paradigm of recessive dystrophic epidermolysis bullosa [J]. Int J Mol Sci, 2021, 22(10):5104.
[6] Jiang P, He S, Li Y, et al. Identification of therapeutic and prognostic biomarkers of lamin C (LAMC) family members in head and neck squamous cell carcinoma [J]. Med Sci Monit, 2020, 26:e925735.
[7] Chamoli A, Gosavi AS, Shirwadkar UP, et al. Overview of oral cavity squamous cell carcinoma: Risk factors, mechanisms, and diagnostics [J]. Oral Oncol, 2021, 121:105451.
[8] Wang X, Xu H, Zhou Z, et al. IGF2BP2 maybe a novel prognostic biomarker in oral squamous cell carcinoma [J]. Biosci Rep, 2022, 42(2):BSR20212119.
[9] Ding C, Yu Z, Li X, et al. Collagen type Ⅶ α1 chain: A promising prognostic and immune infiltration biomarker of pancreatic cancer [J]. Oncol Lett, 2023, 25(2):77.
[10] Oh SE, Oh MY, An JY, et al. Prognostic value of highly expressed type Ⅶ collagen (COL7A1) in patients with gastric cancer [J]. Pathol Oncol Res, 2021, 27:1609860.
[11] Koca D, Séraudie I, Jardillier R, et al. COL7A1 expression improves prognosis prediction for patients with clear cell renal cell carcinoma atop of stage [J]. Cancers (Basel), 2023, 15(10):2701.
[12] Tao Y, Gross N, Fan X, et al. Identification of novel enriched recurrent chimeric COL7A1-UCN2 in human laryngeal cancer samples using deep sequencing [J]. BMC Cancer, 2018, 18(1):248.
[13] Seles M, Hutterer GC, Foβelteder J, et al. Long non-coding RNA PANTR1 is associated with poor prognosis and influences angiogenesis and apoptosis in clear-cell renal cell cancer [J]. Cancers (Basel), 2020, 12(5):1200.
[14] Cave DD, Buonaiuto S, Sainz B Jr, et al. LAMC2 marks a tumor-initiating cell population with an aggressive signature in pancreatic cancer [J]. J Exp Clin Cancer Res, 2022, 41(1):315.
[15] Tong D, Wang X, Liu L, et al. LAMC2 promotes EGFR cell membrane localization and acts as a novel biomarker for tyrosine kinase inhibitors (TKIs) sensitivity in lung cancer [J]. Cancer Gene Ther, 2023, 30(11):1498-1512.
[16] Meireles Da Costa N, Mendes FA, Pontes B, et al. Potential therapeutic significance of laminin in head and neck squamous carcinomas [J]. Cancers (Basel), 2021, 13(8):1890.
[17] Shan F, Liang L, Feng C, et al. LAMC2 regulates proliferation, migration, and invasion mediated by the Pl3K/AKT/mTOR pathway in oral [J]. Oncol Res, 2023, 31(4):481-493.
[18] Saha D, Mitra D, Alam N, et al. Orchestrated expression of vasculogenic mimicry and laminin-5γ2 is an independent prognostic marker in oral squamous cell carcinoma [J]. Int J Exp Pathol, 2022, 103(2):54-64.

COL7A1和LAMC2在口腔鳞状细胞癌中的表达及预后意义

Expression and Prognostic Significance of COL7A1 and LAMC2 in Oral Squamous Cell Carcinoma.

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	庞晓婵, 陈青立, 雒国凤, 尚政军. 牙龈卟啉单胞菌对口腔鳞状细胞癌化疗耐药行为的影响[J]. 口腔医学研究, 2024, 40(5): 413-416.
[2]	梁登桓, 刘家琦, 李东慧, 张佳佳, 郭超杰, 曾妍, 徐江, 郑军. 头颈部鳞状细胞癌中USP10的表达及其预后价值[J]. 口腔医学研究, 2024, 40(5): 417-421.
[3]	付凯钰, 常云汉, 史恩宇, 史澍睿. 共载吲哚菁绿和Nrf2-siRNA的多功能纳米粒子对抗口腔鳞状细胞癌的体外作用研究[J]. 口腔医学研究, 2024, 40(5): 422-428.
[4]	陈嘉雯, 丁啸, 栾可峰, 王高君, 李洪利, 胡温庭, 孙学辉. miR-485-5p靶向RAPTOR影响口腔鳞状细胞癌迁移、侵袭和增殖[J]. 口腔医学研究, 2024, 40(5): 429-435.
[5]	卢芳, 宁彤彤, 王婕, 赵嘉, 房静, 宁倩倩, 李家锋. MYO5A 35号外显子跳跃对头颈鳞状细胞癌患者的预后相关研究[J]. 口腔医学研究, 2024, 40(3): 236-241.
[6]	房敏健, 刘亮, 邱若兰, 曹伟, 葛素云, 柴大敏. 肿瘤间质比在口腔鳞状细胞癌患者预后评估中的价值分析[J]. 口腔医学研究, 2024, 40(3): 242-247.
[7]	李易玮, 孙雅楠, 潘军臣, 胡雅英, 张钰莹, 马纪源, 张佳莉. Barx2驱动口腔鳞状细胞癌肿瘤特异性MHC-Ⅱ类信号诱导CD4⁺T/CD8⁺T细胞活化[J]. 口腔医学研究, 2023, 39(8): 684-689.
[8]	陈河林, 舒佳玉, 董强. CENPN对头颈鳞状细胞癌患者预后的预测价值研究[J]. 口腔医学研究, 2023, 39(8): 690-695.
[9]	陈卓晅, 陈林林. 早期口腔鳞状细胞癌浸润深度检查方法的研究进展[J]. 口腔医学研究, 2023, 39(6): 487-490.
[10]	杨可, 孙雅楠, 胡雅英, 吕宜楠, 郑小风, 李易玮, 张佳莉. cyclin D1表达模式深度学习识别和预测模型对HPV阴性口腔鳞状细胞癌和口咽鳞状细胞癌患者预后的预测作用[J]. 口腔医学研究, 2023, 39(4): 308-315.
[11]	李芷, 陈伟. Notch信号通路在腺样囊性癌中的研究进展[J]. 口腔医学研究, 2023, 39(12): 1042-1045.
[12]	王韧, 周诗洁, 郭锦材. 基于Clinicaltrials.gov平台的口腔鳞状细胞癌临床研究注册特点分析[J]. 口腔医学研究, 2023, 39(12): 1080-1084.
[13]	牛燕红, 夏成万, 章茜, 马冠宇, 王育新, 胡勤刚, 蒲玉梅. 超声加速卢戈氏碘液增强Micro-CT成像用于口腔鳞状细胞癌术中成像的可行性研究[J]. 口腔医学研究, 2023, 39(11): 982-987.
[14]	郎雪莲, 胡涛. 恒牙活髓切断术-从基础到临床的新进展[J]. 口腔医学研究, 2023, 39(10): 866-870.
[15]	吴天赐, 郁佳鑫, 黄晓峰, 陈盛, 王育新, 蒲玉梅. 深度学习图片分类模型ResNet-18用于判定口腔鳞状细胞癌浸润方式的初步研究[J]. 口腔医学研究, 2023, 39(10): 917-922.