超声加速卢戈氏碘液增强Micro-CT成像用于口腔鳞状细胞癌术中成像的可行性研究

doi:10.13701/j.cnki.kqyxyj.2023.11.009

摘要/Abstract

摘要： 目的: 将超声引入卢戈氏碘液(Lugol's iodine)增强Micro-CT成像中,评价其加速口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)样本Micro-CT增强成像的可行性。方法: 首先,超声(3 W)作用于Lugol's iodine(1%、3%、5%)染色Cal-27细胞过程中,作用时间为5、10、15 min。平均光密度值(average optical density, AOD)评价Cal-27染色差异。其次,选择36只Balb/c裸鼠[8周龄,(25±2) g]断颈法处死后分离小鼠舌体组织,按照随机分组分为超声组及无超声组,再以1%、3%、5% Lugol's iodine染色(n=6),在染色后0.5 h和1 h行Micro-CT成像和CT值定量分析。选取南京市口腔医院口腔颌面外科的4例病理诊断为颊黏膜鳞状细胞癌的OSCC男性患者,纳入8块肿瘤样本,随机分为超声组和无超声组。以3%Lugol's iodine浸染组织块,Micro-CT成像时间为染色后0.5 h、1 h、2 h、4 h。CNR值定量分析肿瘤和正常组织间的成像清晰度。结果: 细胞实验结果显示,超声作用Cal-27在5 min和10 min时,1%、3%Lugol's iodine的AOD值高于对照组(P<0.05)。动物实验结果显示,超声作用下小鼠舌体组织的Micro-CT成像的 CT值高于对照组(P<0.05)。临床研究结果显示,OSCC标本超声作用3%Lugol's iodine染色2 h的Micro-CT成像可见肿瘤和正常组织边界, 同时HE切片可见分界线。结论: 超声辅助Lugol's iodine可加速Cal-27的浸润,与此同时超声辅助Lugol's iodine增强Micro-CT成像用于OSCC样本术中切缘评价具有一定的临床可行性。

关键词: 超声, Micro-CT, 卢戈氏碘液, 口腔鳞状细胞癌, 切缘

Abstract: Objective: To evaluate the feasibility of Lugol's iodine enhanced micro-CT imaging accelerated by ultrasound for the oral squamous cell carcinoma (OSCC) samples. Methods: Firstly, ultrasound (3W) was applied to accelerate Lugol's iodine (1%, 3%, and 5%) staining of Cal-27 cell line for 5, 10, and 15 min, respectively. The average optical density (AOD) was used to evaluate the staining difference. Then, thirty-six BALB/C nude mice [8 weeks old, (25±2) g] were randomly divided into ultrasound group and non-ultrasound group, which were stained by 1%, 3%, and 5% Lugol's iodine respectively. Micro-CT imaging and CT quantitative analysis were performed at 0.5 h and 1 h after staining. Finally, four male OSCC patients with buccal mucosa squamous-cell carcinoma were selected and randomly divided into ultrasound group and non-ultrasound group. Micro-CT images were obtained at 0.5 h, 1 h, 2 h, and 4 h after staining with 3% Lugol's iodine, and CNR was used to quantitative analyze the image clarity between tumor and normal tissue. Results: When Cal-27 was exposed to ultrasound for 5 min and 10 min, the AOD of 1% and 3% Lugol's iodine was higher than that of the control group (P<0.05). The value of micro-CT imaging of mouse tongue under ultrasound was higher than that of control group (P<0.05). The OSCC specimen results showed that the tumor and normal tissue boundaries were visible on micro-CT imaging with 3% Lugol's iodine staining for 2 h, while the dividing line was visible on HE sections. Conclusion: Ultrasound-assisted Lugol's iodine accelerates the infiltration of Cal-27, while ultrasound-assisted Lugol's iodine-enhanced micro-CT imaging provides some clinical guidance for intraoperative margin assessment of OSCC specimens.

Key words: ultrasound, micro-CT, Lugol's iodine, oral squamous cell carcinoma, surgical margin

牛燕红, 夏成万, 章茜, 马冠宇, 王育新, 胡勤刚, 蒲玉梅. 超声加速卢戈氏碘液增强Micro-CT成像用于口腔鳞状细胞癌术中成像的可行性研究[J]. 口腔医学研究, 2023, 39(11): 982-987.

NIU Yanhong, XIA Chengwan, ZHANG Qian, MA Guanyu, WANG Yuxin, HU Qingang, PU Yumei. Feasibility Study on Intraoperative Lugol's Iodine Enhanced Micro-CT Imaging Accelerated by Ultrasound for Specimen of Oral Squamous Cell Carcinoma[J]. Journal of Oral Science Research, 2023, 39(11): 982-987.

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