口腔枯草芽孢杆菌代谢产物的抑菌活性研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (10): 991-994.

口腔枯草芽孢杆菌代谢产物的抑菌活性研究

游祥磊¹,王少果¹,曾飒¹,车春晓¹,周建业²,王佳佳²,李志强²,何祥一^1*

1. 兰州大学口腔医学院修复科甘肃兰州 730000;
2. 西北民族大学口腔医学院甘肃兰州 730030

收稿日期:2015-03-20 出版日期:2015-10-28 发布日期:2016-05-04
通讯作者: 何祥一,E-mail: hexy@lzu.edu.cn
作者简介:游祥磊(1988~ ),男,硕士在读,主要从事口腔修复学和口腔微生态学研究。
基金资助:
国家自然科学基金地区项目(编号:31160124/C0309);甘肃省科技计划项目(编号:1204FKCA168、1308RJZA248);甘肃省科技支撑项目(编号:144WCGA167)

In Vitro Inhibitive Activity of Metabolites of Bacillus Subtilis Isolated from Oral Cavity

YOU Xiang-lei¹, WANG Shao-guo¹, ZENG Sa¹, CHE Chun-xiao¹, ZHOU Jian-ye², Wang Jia-jia², LI Zhi-qiang², HE Xiang-yi^1*

1. School of Stomatology, Lanzhou University, Lanzhou 730000, China;
2. School of Stomatology, Northwest University for Nationalities, Lanzhou 730030, China

Received:2015-03-20 Online:2015-10-28 Published:2016-05-04

摘要/Abstract

摘要： 目的:从口腔中分离枯草芽孢杆菌并验证其代谢产物对主要致龋菌的抑制作用。方法:搔刮法提取牙菌斑,平板稀释法筛选出细菌后,进行形态学观察及16S rDNA基因序列的比对。琼脂平板打孔法检测其代谢产物对变形链球菌、粘性放线菌及嗜酸乳杆菌的抑菌活性。结果:从牙菌斑中分离出3株细菌,通过形态学及16S rDNA基因序列检测确定为枯草芽孢杆菌;其代谢产物对变形链球菌、粘性放线菌、嗜酸乳杆菌均具有抑制作用,其中对粘性放线菌抑制作用最强。结论:牙菌斑中可以分离出枯草芽孢杆菌,并且其代谢产物对主要致龋菌具有抑制作用,枯草芽孢杆菌有望作为益生菌在龋病防治中发挥重要作用。

关键词: 枯草芽孢杆菌, 代谢产物, 抑菌活性, 致龋菌

Abstract: Objective: To isolate Bacillus subtilis and verify whether its metabolic product could inhibit the major cariogenic bacteria. Methods: The dental plaque was achieved by scratching method, and Bacillus subtilis were then isolated using plate culture method, which were identified through morphology and 16S rDNA gene sequence analysis. The inhibitory activity of the metabolic products against the major cariogenic bacteria was observed by use of the agar well diffusion method. Results: Three strains of bacteria were isolated from the dental plaque, which were identified as Bacillus subtilis through morphology and 16S rDNA gene sequence analysis. The metabolic products showed significant inhibitory activity to Streptococcus mutans, Viscous actinomycetes and Lactobacillus acidophilus, especially Viscous actinomycetes. Conclusion: Bacillus subtilis can be isolated from the dental plaque, and its metabolic products can inhibit the major cariogenic bacteria. Bacillus subtilis is expected to play an important role in prevention and control of caries disease as an inhibitive bacterium.

Key words: Bacillus subtilis, Metabolic product, Inhibitive activity, Cariogenic bacteria

中图分类号:

R780.1

游祥磊,王少果,曾飒,车春晓,周建业,王佳佳,李志强,何祥一. 口腔枯草芽孢杆菌代谢产物的抑菌活性研究[J]. 口腔医学研究, 2015, 31(10): 991-994.

YOU Xiang-lei, WANG Shao-guo, ZENG Sa, CHE Chun-xiao, ZHOU Jian-ye, Wang Jia-jia, LI Zhi-qiang, HE Xiang-yi. In Vitro Inhibitive Activity of Metabolites of Bacillus Subtilis Isolated from Oral Cavity[J]. Journal of Oral Science Research, 2015, 31(10): 991-994.

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