口腔医学研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (7): 681-683.

• 临床研究论著 • 上一篇    下一篇

牙周炎患者自身组织核酸内NLRP3 mRNA表达水平测定

丁子清,申玉芹,周岳,于海蛟,刘引,高涵,林崇韬*   

  1. 吉林大学口腔医学院牙周病科 吉林 长春 130021
  • 收稿日期:2014-12-15 出版日期:2015-07-28 发布日期:2015-07-28
  • 通讯作者: 林崇韬,电话:0431-85579516
  • 作者简介:丁子清(1988~ ),女,吉林人,硕士在读,主要从事牙周病感染病因学研究。
  • 基金资助:
    国家自然科学基金面上项目(编号:81371153)

Detection of NLRP3 mRNA Expression in Periodontitis Patient's Own Tissue Nucleic Acid

DING Zi-qing, SHEN Yu-qin, ZHOU Yue, et al   

  1. Department of Periodontology, Stomatology Hospital, Jilin University, Changchun 130021
  • Received:2014-12-15 Online:2015-07-28 Published:2015-07-28

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摘要： 目的:通过对牙周炎患者自身组织核酸内炎性小体复合物NLRP3,NLRC4及其相关基因IL-18,IL-1β,Caspase-1等mRNA表达水平的检测,明确炎性小体NLRP3在牙周炎中具有调控作用。方法:采集翻瓣术中慢性牙周炎患者炎性牙周组织以及正畸患者健康牙拔除术获取的健康牙周组织,提取总RNA逆转录cDNA ,-20 ℃冻存备用。Real-time PCR检测NLRP3、NLRC4、IL-18、IL-1β、Caspase-1 mRNA的表达。结果:与健康组牙周组织相比较,牙周炎患者自身组织核酸内NLRP3、IL-18、IL-1β、Caspase-1 mRNA的表达水平明显增高;而NLRC4 mRNA的表达在两组间差异不显著。结论:NLRP3及其相关基因IL-18、IL-1β、Caspase-1 mRNA在牙周炎患者自身组织核酸内的高表达,提示NLRP3在牙周炎的发病机制中具有影响作用。

关键词: 牙周炎患者自身组织细胞核酸, 炎性小体复合物, NLRP3, NLRC4

Abstract: Objective: To determine the mRNA expression of NLRP3,NLRC4, and related genes IL-18,IL-1β,Caspase-1 mRNA in periodontitis patient's own tissue nucleic acid. To define a regulatory role of NLRP3 in periodontitis. Methods: Inflammatory periodontal tissue samples of chronic periodontitis patients were taken in periodontal flap surgery,and healthy gingival tissue samples were taken from orthodontic patients during tooth extractions.Total RNA from gingival tissue was extracted and reverse transcribed into cDNA, cryopreserved for further use. Real-time PCR was used to detect the mRNA expression of NLRP3,NLRC4, related genes IL-18,IL-1β, and Caspase-1 mRNA. Results: The mRNA levels of NLRP3,IL-18,IL-1β,Caspase-1 in the periodontitis patient's own tissue nucleic acid were higher than healthy gingival tissue. However, the mRNA expression of NLRC4 was not significant different between two groups. Conclusion: The high expression of NLRP3 and related genes IL-18, IL-1β, Caspase-1 mRNA in periodontitis patient's own tissue nucleic acid suggested that NLRP3 has an influence on the pathogenesis of periodontitis.

Key words: Periodontitis patient's own tissue nucleic acid, Inflammasomes, NLRP3, NLRC4

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