口腔医学研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (7): 684-687.

• 临床研究论著 • 上一篇    下一篇

蛋白激酶C介导转化生长因子β诱导人口腔鳞癌细胞整合素β6的表达

谷胤征1,2,邓小玲1#,李祺福1,石松林1,陈锡和2,许铭炎1,3*   

  1. 1. 福建省厦门大学医学院 福建 厦门 361102;
    2. 广东省汕头大学医学院;
    3. 福建省厦门市口腔医院
  • 收稿日期:2015-01-26 出版日期:2015-07-28 发布日期:2015-07-28
  • 通讯作者: 许铭炎,E-mail:myxu@xmu.edu.cn
  • 作者简介:谷胤征(1987~ ),男,山东人,硕士,主要从事口腔癌研究工作。邓小玲(1977~ ),女,江西人,副教授,博士,主要从事基因调控及信号传导研究工作。
  • 基金资助:
    国家自然科学基金项目(编号:81072208、81370160、30900661);福建省医学创新课题项目(2014-CXB-47);中央高校基本科研业务费专项资金资助

Protein Kinase C Mediates Transforming Growth Factor β-induced integrin β6 Expression in Human Oral Squamous Cancer Cells

GU Yin-zheng, DENG Xiao-ling, LI Qi-fu, et al   

  1. Medical College of Xiamen University, Xiamen 361102
  • Received:2015-01-26 Online:2015-07-28 Published:2015-07-28

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摘要： 目的:研究蛋白激酶C(PKC)是否参与转化生长因子β(TGF-β)诱导的人口腔鳞癌细胞(SAS)整合素β6表达。方法:人口腔鳞癌细胞SAS 培养于含10%胎牛血清DMEM培养基中,经TGF-β诱导后,裂解细胞提取总RNA和蛋白质,采用实时荧光定量RT-PCR及蛋白印迹方法分别检测整合素β6 mRNA和蛋白表达情况;利用PKC阻断剂研究PKC对整合素β6表达的影响。结果:TGF-β诱导人口腔鳞癌SAS细胞整合素β6 mRNA及蛋白表达呈时间依赖性;广谱型PKC抑制剂RO-31-8220呈剂量依赖性阻断TGF-β诱导的SAS细胞 整合素β6 mRNA和蛋白表达,而Ca2+依赖型PKC抑制剂G6976则没有阻断作用。结论:非Ca2+依赖途径的PKC介导TGF-β诱导人口腔鳞癌细胞SAS细胞整合素β6表达。

关键词: 转化生长因子β, 蛋白激酶C抑制剂, 整合素β6, 人口腔鳞癌细胞

Abstract: Objective: To investigate whether protein kinase C (PKC) is involved in the expression of integrin β6 by human oral squamous cancer cells (SAS) after transforming growth factor β (TGF-β) stimulation. Methods: Human oral squamous cancer cells SAS were cultured with DMEM with 10% fetal bovine serum, after TGF-β stimulation, integrin β6 protein and mRNA levels in the cell lysate were measured by western-blot and quantitative real-time PCR, respectively. Effect of PKC inhibition on integrin β6 expression was investigated by using PKC inhibitors. Results: TGF-β induced SAS integrin β6 mRNA and protein expression levels in a time-dependent manner. These effects of TGF-β could be attenuated by the broad spectrum PKC inhibitor RO-31-8220 in a concentration-dependent manner, whereas Ca2+-dependent PKC inhibitor G6976 had no effect. Conclusion: Ca2+-independent PKC mediates TGF-β-induced integrin β6 expression in cultured SAS cells.

Key words: Transforming growth factor β, Protein kinase C inhibitor Integrin β6, Human oral squamous cancer cells

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