STAT3促进舌鳞癌细胞侵袭与转移的实验研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (7): 688-690.

STAT3促进舌鳞癌细胞侵袭与转移的实验研究

赵艳¹,张壁¹,陈琦¹,王贻宁¹,魏军成^2*

1. 武汉大学口腔医院修复科湖北武汉 430079;
2. 华中科技大学同济医学院附属同济医院妇产科

收稿日期:2014-12-15 出版日期:2015-07-28 发布日期:2015-07-28
通讯作者: 魏军成,E-mail:wjcwjc999@126.com
作者简介:赵艳(1976~ ),女,湖北人,博士,主治医师,主要从事口腔修复科的临床治疗工作。
基金资助:
国家自然科学基金青年项目(编号:81400544 )

STAT3 Promotes Human Lingual Squamous Cell Carcinoma Invasion and Metastasis

ZHAO Yan, ZHANG Bi, CHEN Qi, et al

Department of Prosthodontics, Hospital of Stomatology, Wuhan University, Wuhan 430079

Received:2014-12-15 Online:2015-07-28 Published:2015-07-28

摘要/Abstract

摘要： 目的:研究信号转导和转录激活因子3(STAT3)在人舌鳞状细胞癌组织中的表达,探讨STAT3对舌鳞癌细胞侵袭与转移的促进作用。方法:免疫组织化学染色法检测STAT3在舌鳞状细胞癌组织中的表达,利用小分子干扰RNA(siRNA)体外抑制舌鳞癌细胞系Tca-8113中STAT3的表达,运用体外侵袭实验检测细胞侵袭能力的改变。结果:STAT3表达与肿瘤临床分期(P=0.029)及淋巴结转移(P=0.009)密切相关;siRNA抑制STAT3表达后,细胞侵袭能力明显下降。结论:STAT3促进舌鳞癌细胞侵袭与转移,STAT3 siRNA可以抑制肿瘤细胞的体外侵袭能力。

关键词: 舌鳞状细胞癌, 信号转导和转录激活因子3, 小分子干扰RNA

Abstract: Objective: To investigate the expression of signal transducers and activators of transcription 3 (Stat3) in human lingual squamous cell carcinoma and to evaluate the role of STAT3 for tumor cell invasion and metastasis. Methods: The immunohistochemistry method was used to detect the expression of STAT3 in human lingual squamous cell carcinoma. The human lingual squamous cell carcinoma cell line, Tca-8113 cells were transfected with STAT3 small interfering RNA(siRNA). Transwell assay was used to study cell invasion. Results: There was a significant correlation between STAT3 expression and stages (P=0.029) and lymph node metastasis (P=0.009). The specific knockdown of STAT3 by RNA interference strongly inhibited the motile and invasion activity of tumor cells. Conclusion: STAT3 promotes human lingual squamous cell carcinoma invasion and metastasis. STAT3 siRNA can inhibit the invasion ability of tumor cells.

Key words: Lingual squamous cell carcinoma, STAT3, siRNA

中图分类号:

R739.8

赵艳,张壁,陈琦,王贻宁,魏军成. STAT3促进舌鳞癌细胞侵袭与转移的实验研究[J]. 口腔医学研究, 2015, 31(7): 688-690.

ZHAO Yan, ZHANG Bi, CHEN Qi, et al. STAT3 Promotes Human Lingual Squamous Cell Carcinoma Invasion and Metastasis[J]. Journal of Oral Science Research, 2015, 31(7): 688-690.

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