口腔医学研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (8): 771-.

• • 上一篇    下一篇

载rhPTH(1-34)纳米缓释纤维促成骨作用的体外研究

袁月1,刘童斌1,孟帅岑1,蔡青1,张喆2,王晶晶1,孟维艳1*   

  1. 1. 吉林大学口腔医院口腔种植科 吉林 长春 130021;
    2. 中国科学院长春应化研究所
  • 收稿日期:2015-02-10 出版日期:2015-08-28 发布日期:2016-05-04
  • 通讯作者: 孟维艳,电话:0431-88796025
  • 作者简介:袁月(1990~ ),女,黑龙江人,硕士,主要从事口腔种植学临床及基础的研究工作。
  • 基金资助:
    吉林省发展与改革委员会项目(编号:2013C022-6)

Enhanced Osteogenesis of Polymeric Electrospun Nanofibers Loaded with rhPTH (1-34) in Vitro

YUAN Yue, LIU Tong-bin, MENG Shuai-chen, et al   

  1. School of Stomatology, Jilin University. Changchun 130041
  • Received:2015-02-10 Online:2015-08-28 Published:2016-05-04

PDF (PC)

214

摘要： 目的:制备载甲状旁腺相关肽(rhPTH(1-34))的电纺丝缓释纤维,研究其缓释效果及对成骨细胞增殖、分化的影响。方法:制备含rhPTH(1-34)电纺丝缓释纤维(PLLA/rhPTH(1-34)),扫描电镜(SEM)观察其形貌。紫外分光光度法测定释放速率。噻唑蓝法(MTT)测定PLLA/rhPTH(1-34)对小鼠前成骨细胞(MC3T3-E1)的增值活性, 碱性磷酸酶(ALP)测定其分化。结果:含模型药物的电纺丝缓释纤维体外有效释放21 d,PLLA/rhPTH(1-34)缓释纤维促进细胞的增殖作用明显(P＜0.05),并能显著促进细胞的分化(P＜0.05)。结论:PLLA/rhPTH(1-34)缓释纤维可以有效缓释药物,并能促进MC3T3-E1DE 增殖、分化。

关键词: 电纺丝, 甲状旁腺激素, 小鼠前成骨细胞

Abstract: Objective: To prepare the electrospun fibers which contained parathyroid related peptide (rhPTH (1-34)) and to study its slow release on the proliferation and differentiation of osteoblasts. Methods: Electrospinning fibers (PLLA/rhPTH (1-34)) containing slowing released rhPTH (1-34) were prepared. The appearance and the morphology of the fibers were observed with scanning electron microscopy (SEM). The release kinetics was measured by UV spectrophotometry in vitro. The proliferation activity of MC3T3-E1 cells influenced by PLLA/rhPTH (1-34) was determined through Thiazole blue method (MTT). The differentiation of MC3T3-E1 cells was determined by alkaline phosphatase (ALP). Results: The sustained-release efficiency day of the spinning fibers in vitro was 21. PLLA/rhPTH(1-34) sustained-release fiber could obviously promote the proliferation and differentiation of MC3T3-E1 cells (P<0.05). Conclution: PLLA/rhPTH (1-34) loaded fibers can effectively release drugs and promote the proliferation and differentiation of MC3T3-E1 cells.

Key words: Electrostatic spinning, RhPTH (1-34), MC3T3-E1

中图分类号: