口腔医学研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (8): 767-.

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EGFR参与溶血磷脂酸诱导口腔鳞癌细胞整合素β6的表达

殷豪1,2,邓小玲3#,李国阳4,石松林3,李祺福3,许铭炎1,3*   

  1. 1. 厦门市口腔医院 福建 厦门 361003;
    2. 汕头大学医学院细胞衰老研究室;
    3. 厦门大学医学院基础部;
    4. 辽宁医学院口腔系
  • 收稿日期:2015-01-30 出版日期:2015-08-28 发布日期:2016-05-04
  • 通讯作者: 许铭炎,Email:myxu@xmu.edu.cn
  • 作者简介:殷豪(1990~ ),男,湖南岳阳人,硕士,主要从事癌症相关基因表达调控研究。邓小玲(1977~ ),女,江西人,副教授,博士,主要从事基因调控研究。
  • 基金资助:
    国家自然科学基金项目(81072208,30900661,81370160);福建省卫生系统中青年骨干人才培养项目(2015-ZQN-ZD-35);福建省医学创新课题(2014-CXB-47),中央高校基本业务费专项资金资助;厦门市科技计划项目(3502Z20154072)

EGFR Is Involved in Lysophosphatidic Acid Induced Integrin β6 Expression in Oral Squamous Carcinoma Cells

YIN Hao, DENG Xiao-ling, LI Guo-yang, et al   

  1. Department of Oral Biology and Biomaterial, Xiamen Stomatological Reasearch Institute, Xiamen 36003
  • Received:2015-01-30 Online:2015-08-28 Published:2016-05-04

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摘要： 目的:探讨表皮生长因子受体(EGFR)在溶血磷脂酸(LPA)诱导口腔鳞癌细胞整合素β6(ITGB6)表达中的作用。方法:将口腔鳞癌细胞SAS接种到6孔板中,经LPA诱导后,收集细胞,用定量RT-PCR和Western Blot检测ITGB6的mRNA和蛋白表达量;利用EGFR抑制剂AG1478检测EGFR对LPA诱导ITGB6 mRNA和蛋白表达的影响。结果:1)经10 μmol/L LPA诱导2 h后,SAS细胞ITGB6的mRNA表达水平显著性增加;而ITGB6的蛋白水平则在诱导4h后有显著性升高。2)LPA诱导促进SAS细胞EGFR磷酸化水平增加。3)EGFR抑制剂AG1478抑制LPA诱导SAS细胞ITGB6 mRNA和蛋白表达。结论:LPA能诱导SAS口腔鳞癌细胞ITGB6 mRNA和蛋白表达,EGFR参与LPA诱导的ITGB6表达。

关键词: 溶血磷脂酸, 表皮生长因子受体, 整合素β6, 口腔鳞癌

Abstract: Objective: To investigate the role of EGFR in the process of integrin β6 (ITGB6) expression in oral squamous carcinoma (SAS) cells induced by lysophosphatidic acid (LPA). Methods: SAS cells were cultured in 6-well culture plate and stimulated with 10 μmol/L LPA. The cells were collected for RNA and protein extraction. The mRNA and protein level of ITGB6 were detected by real-time RT-PCR and Western blotting, respectively. The EGFR-specific inhibitor AG1487 was applied to investigate the role of EGFR activation in LPA induced ITGB6 expression.Results: The mRNA level of ITGB6 in SAS cells was statistically upregulated after treated with LPA for 2h, while the protein expression of ITGB6 was obviously increased after incubation with LPA for 4h. The increased phosphorylation of the EGFR was induced by LPA stimulation in SAS cells. EGFR inhibitor AG1478 could block LPA induced ITGB6 mRNA and protein expression in SAS cells. Conclusion: LPA can induce the ITGB6 expression in the oral SAS cells, and EGFR transactivation may be involved in this process.

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