应用QPCR验证口腔黏膜下纤维性变癌变分子标记物的研究

doi:10.13701/j.cnki.kqyxyj.2017.10.002

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (10): 1027-1030.DOI: 10.13701/j.cnki.kqyxyj.2017.10.002

应用QPCR验证口腔黏膜下纤维性变癌变分子标记物的研究

王媛¹，翦新春^1,2*，刘德裕¹

1. 中南大学湘雅医学院附属海口医院口腔医学中心海南海口 570208；
2. 中南大学湘雅医院口腔颌面外科湖南长沙 410008

收稿日期:2017-03-21 出版日期:2017-10-20 发布日期:2017-10-24
通讯作者: 翦新春,E-mail:jianxinchun@hotmail.com
作者简介:王媛(1988~ ),女,贵州人,硕士,主要从事口腔科临床治疗工作。
基金资助:
国家自然科学基金资助项目(编号:281260166)

Identifying Molecular Markers in Oral Squamous Cell Carcinoma Originating from Oral Submucous Fibrosis using QPCR

WANG Yuan ¹,JIAN Xin-chun ^1,2*,LIU De-yu ¹

1. Stomatology Center,the Affiliated Haikou Hospital,Xiangya Medical School,Central South University,Haikou 570208,China;
2. Department of Oral and Maxillofacial Surgery,Xiangya Hospital,Central South University,Changsha 410008,China.

Received:2017-03-21 Online:2017-10-20 Published:2017-10-24

摘要/Abstract

摘要： 目的:探求口腔黏膜下纤维性变(oral submucous fibrosis,OSF)癌变的特异性分子标记物,为阐明口腔黏膜下纤维性变癌变关键机制及早期诊断提供理论基础。方法:基于前期基因芯片的分析数据,筛选出10个候选基因CXCL8、CXCL1、PTPRZ1、WDR66、DKK1、COX6A2、ATP1A2、TPM1、ABCA8、MIB1。采用实时荧光定量核酸扩增检测系统(QPCR)检测在OSF癌变组织12例、非OSF癌变组织10例和正常组织8例中候选基因mRNA的表达。用2^-ΔΔCT法分析比较基因的相对表达量。结果:CXCL1、 PTPRZ1在OSF癌变的颊粘膜组织中表达明显高于非OSF癌变鳞癌组和正常对照组(P<0.05);TMP1在OSF癌变的颊粘膜组织中表达明显低于非OSF癌变鳞癌组和正常对照组(P<0.05)。结论:CXCL1、PTPRZ1、TPM1基因对OSF癌变的早期诊断有意义,可望成为OSF癌变的早期诊断的分子标记物。

关键词: 口腔黏膜下纤维性变, 口腔鳞癌, QPCR

Abstract: Objective: To explore the specific molecular markers of oral squamous cell carcinoma (OSCC) originating from oral submucous fibrosis (OSF), and provide theoretical foundation for clarifying the key mechanism and early diagnosis of OSCC. Methods: Based on the analysis data of early gene chip, 10 candidate genes CXCL8, CXCL1, PTPRZ1, WDR66, DKK1, COX6A2, ATP1A2, TPM1, ABCA8 and MIB1 were screened out. Real-time reverse transcription-polymerase chain reaction (QPCR) was used to detect the expression of candidate genes in 12 OSCC samples originating from OSF, 10 non-OSF OSCC samples, and 8 samples from normal tissues. The results were compared and analyzed using 2^-ΔΔCTequation.Results: The expression of CXCL1 and PTPRZ1 were significantly higher than that of the non-OSF and normal control group (P<0.05). The expression of TPM1 was significant lower than that of the non-OSF and normal control group (P<0.05). Conclusion: CXCL1, PTPRZ1, and TPM1 are meaningful to the early diagnosis of OSCC originating from OSF, expected to become specific molecular markers of the early diagnosis.

Key words: Oral submucous fibrosis , Oral squamous cell carcinoma, QPCR

中图分类号:

R739.8

王媛，翦新春，刘德裕. 应用QPCR验证口腔黏膜下纤维性变癌变分子标记物的研究[J]. 口腔医学研究, 2017, 33(10): 1027-1030.

WANG Yuan ,JIAN Xin-chun ,LIU De-yu. Identifying Molecular Markers in Oral Squamous Cell Carcinoma Originating from Oral Submucous Fibrosis using QPCR[J]. Journal of Oral Science Research, 2017, 33(10): 1027-1030.

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应用QPCR验证口腔黏膜下纤维性变癌变分子标记物的研究

Identifying Molecular Markers in Oral Squamous Cell Carcinoma Originating from Oral Submucous Fibrosis using QPCR

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