新型复合弓丝抗变形链球菌粘附的研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (9): 876-879.

新型复合弓丝抗变形链球菌粘附的研究

张兵¹,于文雯¹,张超²,刘继明¹,孙晗¹,孙新华^1*

1. 吉林大学口腔医学院正畸科吉林长春 130021;
2. 南方医科大学附属口腔医院·广东省口腔医院正畸科

收稿日期:2015-01-28 出版日期:2015-09-28 发布日期:2016-05-04
通讯作者: 孙新华,E-mail:xinhuasun8@163.com
作者简介:张兵(1988~ ),女,河南人,硕士,主要从事正畸矫治弓丝的材料学研究。

Anti-adhesion Study of Laser Welding Composite Arch Wire to Streptococcus Mutans

ZHANG Bing¹,YU Wen-wen¹,ZHANG Chao²,LIU Ji-ming¹, SUN Han¹, SUN Xin-hua¹

1. Department of Orthodontics, School of Stomatology, Jilin University, Changchun 130021, China;
2. Department of Orthodontics, Guangdong Provincial Stomatology Hospital, Guangzhou 510000, China

Received:2015-01-28 Online:2015-09-28 Published:2016-05-04

摘要/Abstract

摘要： 目的:采用荧光显微镜和扫描电镜,观察激光焊接加铜中间层镍钛-不锈钢复合弓丝(Composite Arch wire,CAW)的抗变形链球菌粘附作用。方法:将未与变形链球菌共培养的镍钛弓丝、不锈钢弓丝、复合弓丝及铜丝组分别作为空白对照组;将与变形链球菌共培养的镍钛弓丝、不锈钢弓丝、复合弓丝及铜丝组分别作为实验组,经共培养48 h,进行荧光显微镜与扫描电镜观察。结果:实验组与空白对照组经荧光显微镜观察,显示复合弓丝组加铜中间层处与铜丝表面均呈红色荧光区;扫描电镜观察显示实验组复合弓丝组加铜中间层处与铜丝细菌粘附均少于镍钛弓丝、不锈钢弓丝及复合弓丝的镍钛侧及不锈钢侧;且变形链球菌形态出现溶解与破碎现象。 结论:复合弓丝组加铜中间层处对变形链球菌具有一定的抗粘附作用。

关键词: 复合弓丝, 激光焊接, 变形链球菌, 抗粘附

Abstract: Objective: To study the anti-adhesive characteristics of composite arch wire of lased-welded nickel titanium arch wire and stainless steel arch wire with pure copper interlayer (CAW) with fluorescence microscope and scanning electron microscope. Methods: The nickel titanium arch wire, stainless steel arch wire copper wire and CAW unco-cultured with streptococcus mutans suspension were selected as experimental group. The nickel titanium arch wire, stainless steel arch wire copper wire and CAW co-cultured with streptococcus mutans suspension were selected as control group. The surface morphology was detected with fluorescent staining and scanning electron microscope after 48 hours. Results: The copper interlayer of CAW and copper wire showed red fluorescence of none bacterial adhesion area in experimental grous and control grous with fluorescent microscope. Less streptococcus mutans adhered to the copper interlayer of CAW than the nickel titanium arch wire,stainless steel arch wire, and composite arch wire nickel titanium parts, stainless steel parts. At the same time, the streptococcus mutans became dissolved and broken. Conclusion: The copper interlayer of CAW has certain anti-adhesive effect on streptococcus mutans.

Key words: Composite arch wire, Laser weld, Streptococcus mutans, Anti-adhesion

中图分类号:

R783.5

张兵,于文雯,张超,刘继明,孙晗,孙新华. 新型复合弓丝抗变形链球菌粘附的研究[J]. 口腔医学研究, 2015, 31(9): 876-879.

ZHANG Bing,YU Wen-wen,ZHANG Chao,LIU Ji-ming, SUN Han, SUN Xin-hua. Anti-adhesion Study of Laser Welding Composite Arch Wire to Streptococcus Mutans[J]. Journal of Oral Science Research, 2015, 31(9): 876-879.

参考文献

[1] 朱宪春,孙新华,欧阳喈,等.复合矫治弓丝矫治错位牙的受力分析[J] .现代口腔医学杂志,2005,19(1)∶19-23
[2] 孙新华,朱宪春,孙大谦,等.镍钛-不锈钢复合矫治弓丝在正畸拔牙病例中的初步应用[J] .口腔正畸学,2005,12(2)∶53-56
[3] 李红梅.TiNi形状记忆合金与不锈钢异种材料激光焊接研究[D] .长春:吉林大学,2011
[4] Li Hongmei,Sun Daqian,Han Yaowu,et al. Microstructures and mechanical properties of laser-welded TiNi shape memory alloy and stainless steel wires [J] . China Welding, 2010, 19(3)∶1-5
[5] Sun DQ,Li HM. A new method of TiNi shape memory alloy and austenitic stainless steel different Kind of material connection [J] . CN Patent, 2011, CNIO 215201708
[6] 于文雯,张超,张兵,等.镍钛-不锈钢激光焊复合弓丝细胞毒性的检测[J] .中华口腔医学杂志,2014,49(4)∶239-243
[7] Chao Zhang,Xinhua Sun.Susceptibility to Stress Corrosion of Laser-Welded Composite Arch Wire in Acid Artificial Saliva [J] . Advances in Materials and Engineering,Volume, 2013, Article ID 738954, 8 pages
[8] Chao Zhang,Xinhua Sun,Xu Hou,et al. The Corrosion Resistance of Composite Arch Wire Laser-Welded By NiTi Shape Memory Alloy and StainlessSteel Wires with CuInterlayer in Artificial Saliva with Protein [J] . International Journal of Medical Sciences,2013,10(8)∶1068-1072
[9] Chao Zhang,Xinhua Sun,Shuang Zhao,et al. Susceptibility to Corrosion and In Vitro Biocompatibility of a Laser-Welded Composite Orthodontic Arch Wire [J] . Annals of Biomedical Engineering, 2014, 42(1)∶222-230
[10] Chao Zhang,Xinhua Sun,Xiumei Sun,et al. Corrosion of laser-welded NiTi shape memory alloy and stainlesssteel composite wires with a copper interlayer upon exposure to fluorideand mechanical stress [J] . Corrosion Science,2014,82(5)∶404-409
[11] Sharifahmadian O, Salimijazi H R, Fathi M H, et al. Relationship between surface properties and antibacterial behavior of wire arc spray copper coatings [J] . Surface and Coatings Technology, 2013, 233∶74-79
[12] 杨伟超,南黎,李慧,等.含铜抗菌不锈钢的抗菌性能研究[J] .微生物学杂志,2009 (3)∶1-5
[13] Shirai T, Tsuchiya H, Shimizu T, et al. Prevention of pin tract infection with titanium-copper alloys [J] . Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2009, 91(1)∶373-380
[14] Robert Duduid. Copper-inhibition of the growth of oral streptococci and actinomyces [J] . Biumaterials, 1983, 4(7)∶225-227
[15] Ristic M,Vlahovic Svabic M,Sasic M,et al. Clinical and microbiological effects of fixed orthodontic appliances on periodontal tissues in adolescents [J] . Orthodontics & craniofacial Research,2007,10(4)∶187-195
[16] Rosenbloom RG,Tinanoff N. Salivary streptococcus mutans levels in patients before during and after orthodontic treatment [J] . American Journal of Orthodontics and Dentofacial Orthopedics,1991, 100(1)∶35-37
[17] 陈四红,吕曼祺,张敬党,等.含 Cu 抗菌不锈钢的微观组织及其抗菌性能[J] .金属学报,2004,40(3)∶314-318
[18] R.L. Stardey. Effect of pH on Toxicity of Copper to Scytalidium sp., a Copper-tolerant Fungus, and Some Other Fungi [J] . Journal of General Microbiology,1973,78(2)∶217-225
[19] Dan ZG,Ni HW, Xu BF, et al. Microstructure and antibacterial properties of AISI 420 stainless steel implanted by copper ions [J] . Thin Solid Films,2005,492(1)∶93-100
[20] Garrett T R, Bhakoo M, Zhang Z. Bacterial adhesion and biofilms on surfaces [J] . Progress in Natural Science, 2008, 18(9)∶1049-1056