口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (3): 249-252.DOI: 10.13701/j.cnki.kqyxyj.2016.03.009

• 基础研究论著 • 上一篇    下一篇

乳铁蛋白对牵张成骨促进作用的研究

赵芮1, 李汶洋2, 胡静1   

  1. 1. 四川大学华西口腔医学院正颌与颞下颌关节外科 四川 成都 610041;
    2. 重庆医科大学口腔医学院 重庆 400016
  • 收稿日期:2015-09-11 出版日期:2016-03-28 发布日期:2016-03-29
  • 通讯作者: 胡静,电话:028-85502334
  • 作者简介:赵芮(1989~ ),男,北京人,硕士,主要从事牵张成骨的临床及基础研究。
  • 基金资助:
    高等学校博士学科点专项科研基金(编号:20130181130004130101004100)

Effects of Lactoferrin on Promoting Bone Regeneration during Distraction Osteogenesis.

ZHAO Rui1, LI Wen-yang2, HU Jing1*   

  1. 1. West China College of Stomatology, Sichuan University, Chengdu 610041, China;
    2. College of Stomatology, Chongqing Medical University, Chongqing 400016, China
  • Received:2015-09-11 Online:2016-03-28 Published:2016-03-29

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摘要： 目的: 研究乳铁蛋白(LF)对牵张成骨的作用。方法: 将40只新西兰大白兔随机分为对照组(20只)和实验组(20只),建立牵张成骨动物模型。对照组自延迟期第2天开始每日灌胃0.9%氯化钠溶液(1 ml/kg),相同时间内,将LF(85 mg/kg)溶解于相同容积的0.9%氯化钠溶液中对实验组进行灌胃。于牵张结束后8周处死所有实验动物,收集标本进行X线、Mirco-CT、组织学和生物力学检测。结果: 实验组动物牵张间隙内新骨形成的量优于对照组,并显著改善了新生骨痂的显微结构及生物力学强度。结论: LF能够促进牵张成骨过程中新骨的形成。

关键词: 牵张成骨, 乳铁蛋白, 骨再生

Abstract: Objective: To investigate the effects of lactoferrin (LF) on distraction osteogenesis (DO). Methods: Forty New Zealand white rabbits were used to establish the DO animal model, and randomly divided into control group (n=20) and experimental group (n=20). At the beginning of distraction, the rabbits were daily administrated by gavage with LF (85 mg/kg) in experimental group, while 0.9% physiological saline (1 mL/kg) in control group. At the 8th week after finishing the distraction, animals were euthanatized, and the samples were collected and preceded to the X-ray, micro-CT, histological, and biomechanical examinations. Results: The radio-density of experimental group was much higher than that of control group. In addition, LF treatment increased the microstructure and mechanical strength of the newly formed bone. Conclusion: LF could promote the bone regeneration during the process of DO.

Key words: Distraction osteogenesis, Lactoferrin , Bone regeneration

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