口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (10): 1044-1047.DOI: 10.13701/j.cnki.kqyxyj.2017.10.006

• 基础研究论著 • 上一篇    下一篇

甲状旁腺素相关肽核定位序列和C-末端缺失对小鼠下颌骨发育的影响

刘洪*，颜成东，蒋尚飞   

  1. 江苏医药职业学院 江苏 盐城 224005
  • 收稿日期:2017-04-16 出版日期:2017-10-20 发布日期:2017-10-24
  • 通讯作者: 刘洪,E-mail: liuhongsy@ycmc.edu.cn
  • 作者简介:刘洪(1975~ ),男,江苏盐城人,副教授,硕士,研究方向为口腔硬组织发育和矿化。
  • 基金资助:
    盐城市医学科技发展计划项目(编号:YK2016049)

Influence of Nuclear Localization Sequence and C-Terminal Deletion of Parathyroid Hormone Relative Peptide on Mandible Development in Mice

LIU HONG*, YAN Cheng-dong , JIANG Shang-fei   

  1. Vocational Collagen of Medicine, Yancheng 224005, China.
  • Received:2017-04-16 Online:2017-10-20 Published:2017-10-24

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摘要： 目的:研究甲状旁腺素相关肽(parathyroid hormone relative peptide, PTHrP)核定位序列和C-末端缺失对小鼠下颌骨发育的影响。方法:使用CT扫描三维重建、HE染色、总胶原染色、碱性磷酸酶(alkaline phosphatase,ALP)染色、抗酒石酸酸性磷酸酶(tartrate resistant acid phosphatase, TRAP)染色、免疫组织化学染色和western-blot等方法对PTHrP敲入(PTHrP knock in,PTHrP KI)小鼠(PTHrP KI 组,n=10)和野生型(wild type,WT)小鼠的下颌第一磨牙的差异。结果:WT小鼠成骨细胞密度、下颌骨相对骨量、ALP阳性面积、一型胶原(collagen-I)阳性面积均明显高于PTHrP KI小鼠,并且差异均具有统计学意义(P<0.05);而PTHrP KI小鼠下颌骨p16,p21和p27蛋白表达水平明显高于WT小鼠,Ki-67蛋白表达水平低于WT小鼠,并且差异均具有统计学意义(P<0.05)。结论:PTHrP核定位序列和C-末端缺失导致了成骨细胞减少,抑制了胞外基质的合成和矿化,从而导致了小鼠下颌骨的发育障碍。

关键词: 甲状旁腺素相关肽, 核定位序列, C末端, 下颌骨, 发育

Abstract: Objective: To study the influence of nuclear localization sequence (NLS) and C-terminal deletion of parathyroid hormone relative peptide (PTHrP) on the mandible development in mice. Methods: Computed tomography (CT) scanning, hematein eosin (HE) staining, total collagen staining, alkaline phosphatase (ALP) staining, tartrate-resistant acid phosphatase (TRAP) staining, immunohistochemical staining, and western-blot were used to compare the developmental difference of the first molar in mandible between PTHrP knock mice (PTHrP KI group, n=10) and wild type mice (WT group). Results: Osteoblast density, mandible relative bone mass, ALP positive area, and collagen-I positive area of WT group were markedly higher than PTHrP KI group (P<0.05). However, expressions of mandible p16, p21, and p27 proteins of PTHrP KI group were higher than that of the WT group, while Ki-67 protein was lower than that of the WT group, and the differences were statistical significant (P<0.05). Conclusion: NLS and C-terminal deletion of PTHrP induce the decrease of osteoblasts and inhibit the synthesis and mineralization of extracellular matrix, thus leading to the mandible development disorder in mice.

Key words: Parathyroid hormone, related peptide, Nuclear localization sequence, C-terminal, Mandible, Development

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