口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (11): 1172-1176.DOI: 10.13701/j.cnki.kqyxyj.2018.11.007

• 龋病牙髓病学研究 • 上一篇    下一篇

新疆博州地区维吾尔族、汉族儿童乳酸杆菌的分布及其与龋病的相关性研究

董英, 曹宏飞, 杨婷, 李贝贝, 赵今*   

  1. 新疆医科大学第一附属医院牙体牙髓科,新疆维吾尔自治区口腔医学研究所 新疆 乌鲁木齐 830054
  • 收稿日期:2018-05-06 出版日期:2018-11-28 发布日期:2018-11-23
  • 通讯作者: 赵今,E-mail:merryljin@sina.com
  • 作者简介:董英(1994~ ),女,河南人,硕士在读,主要从事龋病防治研究工作。
  • 基金资助:
    国家自然科学基金(编号:81760194)

Distribution of Lactobacillus of the Uygur and Han Children in Bo Zhou, Xinjiang and Its Correlation with Caries

DONG Ying, CAO Hong-fei, YANG Ting, LI Bei-bei, ZHAO Jin*   

  1. The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China
  • Received:2018-05-06 Online:2018-11-28 Published:2018-11-23

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摘要： 目的:研究新疆博州地区汉族、维吾尔族儿童口腔内乳酸杆菌的分布及其与龋病的相关性。方法:选用分层整群随机抽样方法抽取722名3~5岁健康儿童作为研究对象,分为3组:重度低龄儿童龋儿童281名,低龄儿童龋儿童283名,无龋儿童209名。采集牙菌斑样本,用Rogosa和MRS培养基选择性培养分离乳酸杆菌,经革兰染色、生化实验及聚合酶链式反应等方法进一步鉴定。Pearson χ2检验用于分类资料的统计分析。结果:总体样本中,乳酸杆菌检出率为63.2%;汉族儿童乳酸杆菌检出率69.5%高于维吾尔族58.2%(P=0.001);重度低龄儿童龋组乳酸杆菌检出率75.4%高于低龄儿童龋组66.8%和无龋40.4%(P=0.000);男性儿童乳酸杆菌检出率62.6%,女性63.8%,性别分布差异无统计学意义(P=0.794);3岁儿童乳酸杆菌检出率57.5%,4岁儿童65.2%与5岁儿童65.9%,差异无统计学意义(P=0.119)。结论:口腔内乳酸杆菌的分布存在民族差异;重度低龄儿童龋组、低龄儿童龋组和无龋组的乳酸杆菌分布有显著性差异;乳酸杆菌可能是龋病发生发展中的危险因素。

关键词: 儿童龋病, 乳酸杆菌, 少数民族, 聚合酶链式反应

Abstract: Objective: To investigate the distribution of oral lactobacillus and its relationship with caries among 3-5 years old children of Uygur and Han nationalities in Bo Zhou area. Methods: Totally 722 healthy children aged 3 to 5 years were selected with a stratified cluster random sampling method. Dental plaque samples were collected, and lactobacillus was cultured selectively through Rogosa and MRS agar medium. The isolates were further identified by methods of gram staining, hydrogen peroxide test, and polymerase chain reaction molecular biology methods. Pearson χ2 test was used for statistical analysis of classification data. Results: The prevalence of lactobacillus were 69.5% in Han children and 58.2% in Uygur children, respectively (P=0.001). The tested rate of lactobacillus in severe early childhood caries children group (75.4%) was significantly higher than that of early childhood caries children group (66.8%) and caries-free group (40.4%) (P=0.000). Lactobacillus could be isolated from 62.6% boys and girls of Uygur children and 63.8% of Han children (P=0.794) in the overall samples. The prevalence of lactobacillus was 57.5% in 3 years old children, 65.2% in 4 years old children, and 65.9% in 5 years old children, respectively (P=0.119). Conclusion: There was difference in the distribution of oral lactobacillus between Uygur and Han ethnic groups. There were significant differences in prevalence of lactobacillus between groups of severe early childhood caries, early childhood caries, and caries-free children. Lactobacillus may be a risk factor in the development of caries.

Key words: Dental caries, Lactobacillus, Minority ethnicity, Polymerase chain reaction