HtrA对乳牙变异链球菌高毒力株gbpC表达的影响

doi:10.13701/j.cnki.kqyxyj.2020.01.015

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (1): 61-64.DOI: 10.13701/j.cnki.kqyxyj.2020.01.015

HtrA对乳牙变异链球菌高毒力株gbpC表达的影响

韩林秀, 王望, 王玲, 杨维虹, 刘瑶, 刘兴容^*

西南医科大学附属口腔医院儿童口腔科西南医科大学口颌面修复重建和再生实验室四川泸州 646000

收稿日期:2019-04-04 出版日期:2020-01-28 发布日期:2020-01-16
通讯作者: 刘兴容,E-mail:liuxingrong163@163.com
作者简介:韩林秀(1993~ ),女,重庆人,硕士在读,主要从事牙体牙髓病的基础与临床研究。
基金资助:
四川省科技厅-泸州市人民政府-泸州医学院2014年联合科研项目(编号:LY53)四川省卫生厅科研基金(编号:100247)四川省教育厅科研基金(编号:13ZA0235)泸州医学院科研基金(编号:4)

Effect of HtrA on Expression of gbpC of Streptococcus Mutans Isolated from Children with High Cario-susceptibility.

HAN Linxiu, WANG Wang, WANG Ling, YANG Weihong,LIU Yao, LIU Xingrong^*

Department of Pediatric Dentistry,the Affiliated Stomatology Hospital of southwest Medical University,Oral & Maxillofacial Reconstruction and Regeneration Laboratory,Southwest Medical University,Luzhou 646000,china

Received:2019-04-04 Online:2020-01-28 Published:2020-01-16

摘要/Abstract

摘要： 目的:在体外非应激条件下,研究乳牙变异链球菌(streptococcus mutans, S.mutans)高温需要A蛋白(high temperature requirement serine proteinase A,HtrA)基因缺陷株和HtrA高毒力株的葡聚糖结合蛋白C(glucan-binding protein C, gbpC)表达能力的差异,探讨HtrA基因对gbpC表达的影响。方法:选用前期已获得的乳牙HtrA高毒力株和高致龋性HtrA基因缺陷株,在BHI培养基培养至指数期第12 h后进行细菌革兰染色及生化鉴定。以qPCR和Western Blot分别检测两组S.mutans菌株中gbpC基因和蛋白的表达情况。结果:HtrA高毒力株相对HtrA基因缺陷株具有更高的gbpC基因和蛋白表达量。结论:S.mutans gbpC的表达会受HtrA的影响。HtrA基因可能是S.mutans高致龋性的重要因素之一。

关键词: 变异链球菌, 生长曲线, 高温需要A蛋白, 葡聚糖结合蛋白C

Abstract: Objective: To compare the expression of glucan-binding protein C (gbpC) of streptococcus mutans under normal outside environment between high temperature requirement serine proteinase A (HtrA) gene-deficient strains and high-virulent strains of streptococcus mutans (S.mutans) isolated from children with high cario-susceptibility. Methods: The HtrA-deficient strains and high virulent strains of S.mutaus were obtained by preliminary study. The strains were reanimated and incubated in BHI medium to exponential phase at 12th hour. The biochemical identification and Gram stain identification were used to identify them. The expression of gbpC gene and protein in S.mutans strains were detected by qPCR and Western Blot, respectively. Results: HtrA highly virulent strains had higher gbpC gene and protein expression levels than that of HtrA gene-deficient strains. Conclusion: The expression of gbpC in streptococcus mutans was affected by HtrA

Key words: streptococcus mutans, growth curve, HtrA, gbpC

韩林秀, 王望, 王玲, 杨维虹, 刘瑶, 刘兴容. HtrA对乳牙变异链球菌高毒力株gbpC表达的影响[J]. 口腔医学研究, 2020, 36(1): 61-64.

HAN Linxiu, WANG Wang, WANG Ling, YANG Weihong,LIU Yao, LIU Xingrong. Effect of HtrA on Expression of gbpC of Streptococcus Mutans Isolated from Children with High Cario-susceptibility.[J]. Journal of Oral Science Research, 2020, 36(1): 61-64.

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HtrA对乳牙变异链球菌高毒力株gbpC表达的影响

Effect of HtrA on Expression of gbpC of Streptococcus Mutans Isolated from Children with High Cario-susceptibility.

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