口腔医学研究

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (6): 572-576.DOI: 10.13701/j.cnki.kqyxyj.2020.06.016

• 牙周病学研究 • 上一篇    下一篇

烟草提取物辅酶Q10对牙周炎小鼠Treg/T17细胞免疫平衡的影响

吴芸菲1, 张黎2, 张旭凤2, 符起亚2*   

  1. 1.海南医学院第一附属医院口腔科 海南 海口 570102;
    2.海南医学院口腔医学院 海南 海口 570102
  • 收稿日期:2019-09-07 出版日期:2020-07-03 发布日期:2020-07-06
  • 通讯作者: *符起亚,E-mail:283172396@qq.com
  • 作者简介:吴芸菲(1981~ ),女,海南海口人,本科,主治医师,主要从事口腔内科学研究工作。
  • 基金资助:
    海南省高等学校科学研究项目(编号:Hnky2018ZD-7)

Effect of Tobacco Extract Coenzyme Q10 on Immunologic Balance of Treg/T17 Cells in Mice with Periodontitis

WU Yunfei1, ZHANG Li2, ZHANG Xufeng2, FU Qiya2*   

  1. 1. Department of Stomatology, the First Affiliated Hospital of Hainan Medical University, Haikou 570102, China;
    2. School of Stomatology, Hainan Medical University, Haikou 570102, China
  • Received:2019-09-07 Online:2020-07-03 Published:2020-07-06

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摘要： 目的: 探讨烟草提取物辅酶Q10(CoQ10)对牙周炎小鼠Treg/T17细胞免疫平衡的影响。方法: 将36只健康雄性小鼠分为对照组、模型组、替硝唑组及CoQ10低、中、高剂量组,每组6只,灌胃6周。牙周炎小鼠模型采用结扎法建立。检测小鼠牙槽骨丧失(ABL)值和牙槽骨吸收情况,苏木素-伊红(HE)染色检测小鼠牙周组织学变化,流式细胞仪分析小鼠外周血单个核细胞(PBMC)中CD4+T细胞、CD4+Treg细胞和CD4+T17细胞比例变化,ELISA法检测小鼠血清中TGF-β1、IL-10,IL-17水平。结果: 与对照组比较,模型组、替硝唑组、CoQ10各剂量组ABL值均显著升高(P＜0.05);与模型组比较,替硝唑组和CoQ10各剂量组ABL值均显著下降(P＜0.05);与模型组比较,替硝唑组和CoQ10各剂量组牙周膜纤维排列较为整齐,结缔组织内炎性细胞浸润程度减轻;与对照组比较,模型组小鼠外周血PBMC中CD4+T细胞、CD4+T17细胞百分比及IL-17A水平升高(P＜0.05)、CD4+Treg细胞百分比及TGF-β、IL-10水平降低(P＜0.05);与模型组比较,替硝唑组和CoQ10各剂量组CD4+T细胞、CD4+T17细胞百分比及IL-17A水平降低(P＜0.05)、CD4+Treg细胞百分比及TGF-β、IL-10水平升高(P＜0.05)。结论: 烟草提取物CoQ10能够调节小鼠Treg/T17细胞免疫平衡,对牙周炎起到改善作用。

关键词: 牙周炎小鼠, 烟草提取物, 辅酶Q10, Treg细胞, T17细胞, 免疫平衡

Abstract: Objective: To investigate the effect of tobacco extract coenzyme Q10 (CoQ10) on the immunologic balance of Treg/T17 cells in mice with periodontitis. Methods: 36 healthy male mice were divided into control group, model group, tinidazole group, CoQ10 low dose group, CoQ10 medium dose group, and CoQ10 high dose group, with 6 rats in each group, and were given gavage for 6 weeks. The mice model of periodontitis was established by ligation. Alveolar bone loss (ABL) value and alveolar bone resorption were measured in mice. Hematoxylin-eosin (HE) staining was used to detect periodontal histological changes in mice. Flow cytometry was used to analyze the proportions of CD4+ T cells, CD4+ Treg cells, and CD4+ T17 cells in peripheral blood mononuclear cells (PBMC) of mice. The levels of serum TGF-β1, IL-10, and IL-17 were detected by ELISA. Results: Compared with the control group, the ABL values of model group, tinidazole group, and various CoQ10 groups were significantly higher (P<0.05). Compared with the model group, the ABL values of tinidazole group and various CoQ10 groups decreased significantly (P<0.05). Compared with the model group, the periodontal ligament fibers in tinidazole group and various CoQ10 groups were arranged neatly, and the inflammatory cell infiltration in connective tissues was reduced. Compared with the control group, the percentages of CD4+ T cells, CD4+ T17 cells, and the level of IL-17A in peripheral blood PBMC of model group mice increased (P<0.05), and the percentage of CD4+ Treg cells and the levels of TGF-β and IL-10 decreased (P<0.05). Compared with model group, the percentage of CD4+ T cells, CD4+ T17 cells, and the level of IL-17A in tinidazole group and CoQ10 groups decreased (P<0.05), and the percentage of CD4+ Treg cells and the levels of TGF-β and IL-10 increased (P<0.05). Conclusion: Tobacco extract CoQ10 can regulate the immune balance of Treg/T17 cells in mice and alleviate periodontitis.

Key words: periodontitis mice, tobacco extract, coenzyme Q10, Treg cells, T17 cells, immunologic balance