生物节律紊乱对小鼠牙本质发育的影响

doi:10.13701/j.cnki.kqyxyj.2022.08.007

口腔医学研究 ›› 2022, Vol. 38 ›› Issue (8): 731-735.DOI: 10.13701/j.cnki.kqyxyj.2022.08.007

生物节律紊乱对小鼠牙本质发育的影响

李瑞琪, 黄妩双, 李实语, 郑雪晴, 刘念柯, 宋亚玲^*

口腔基础医学省部共建国家重点实验室培育基地和口腔生物医学教育部重点实验室,武汉大学口腔医学院湖北武汉 430079

收稿日期:2021-12-07 出版日期:2022-08-28 发布日期:2022-08-24
通讯作者: *宋亚玲,E-mail: sningya@whu.edu.cn
作者简介:李瑞琪(1996~ ),女,河南开封人,硕士在读,研究方向:牙发育。
基金资助:
国家自然科学基金(编号:81670976)

Influence of Circadian Disruption on Dentin Development in Mice

LI Ruiqi, HUANG Wushuang, LI Shiyu, ZHENG Xueqing, LIU Nianke, SONG Yaling^*

Hubei-MOST KLOS & KLOBM, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China

Received:2021-12-07 Online:2022-08-28 Published:2022-08-24

摘要/Abstract

摘要： 目的: 探究生物节律紊乱对牙本质发育的影响。方法: 体外分离培养小鼠牙乳头细胞并进行同步化处理,实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)检测生物钟控基因Bmal1、Per2连续48 h波动情况。构建环境性节律紊乱小鼠模型,通过免疫组织化学(immunohistochemistry,IHC)检测BMAL1、PER2、DSPP表达;通过扫描电镜观察、场发射电子探针和显微硬度测试分别检测正常组和紊乱组小鼠牙本质结构、化学元素组成及硬度变化。结果: Bmal1和Per2在小鼠牙乳头细胞中呈节律性表达。环境性生物节律紊乱使小鼠成牙本质细胞中BMAL1、PER2、DSPP表达减弱。节律紊乱组小鼠下颌切牙牙本质钙含量以及钙磷比相较于对照组明显降低(P<0.05),牙本质小管结构、数量及牙本质硬度较对照组无明显差异。结论: 生物节律可能参与并调控牙本质发育。

关键词: 牙乳头细胞, 节律基因, 节律紊乱

Abstract: Objective: To investigate the effect of circadian disruption during dentin formation. Methods: The model of environmental circadian disruption was constructed in mice and immunohistochemistry (IHC) was employed to detect the expression of BMAL1, PER2, and DSPP in molar germs in offspring of pregnant mice. Mouse dental papilla cells (mDPCs) were separated from Kunming mice at postnatal day 1 (PN1). After synchronization, the cells were cultured for 48h and harvested every 6h. qRT-PCR was used to explore the expression of Bmal1 and Per2. Scanning electron microscope (SEM) was used to compare the structure of dentinal tubules in the sagittal plane of incisor and molar in control and disturbance group. Energy-dispersive X-ray spectroscopy (EDS) was performed for surface elements analysis of mandibular incisors. The mineral density of dentin in both groups was compared and analyzed. Results: The expression of BMAL1, PER2, and DSPP decreased in the mandibular first molar germs of circadian rhythm disturbance group compared with the control group. Moreover, during 48h period, Bmal1 and Per2 exhibited a rhythmic profile in synchronized mouse dental papilla cells. The element weight percent of Ca and the value of the Ca/P ratio were reduced in disturbance group compared with those in the control group. The results of SEM and mineral density of dentin exhibited no obvious abnormalities. Conclusion: Circadian rhythm might involve in regulating dentin development.

Key words: dental papilla cells, circadian gene, circadian disruption

李瑞琪, 黄妩双, 李实语, 郑雪晴, 刘念柯, 宋亚玲. 生物节律紊乱对小鼠牙本质发育的影响[J]. 口腔医学研究, 2022, 38(8): 731-735.

LI Ruiqi, HUANG Wushuang, LI Shiyu, ZHENG Xueqing, LIU Nianke, SONG Yaling. Influence of Circadian Disruption on Dentin Development in Mice[J]. Journal of Oral Science Research, 2022, 38(8): 731-735.

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生物节律紊乱对小鼠牙本质发育的影响

Influence of Circadian Disruption on Dentin Development in Mice

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