牙釉质表面气相SiO2/PDMS超疏水涂层制备及其细胞毒性与抗菌性能评价

doi:10.13701/j.cnki.kqyxyj.2022.08.006

口腔医学研究 ›› 2022, Vol. 38 ›› Issue (8): 725-730.DOI: 10.13701/j.cnki.kqyxyj.2022.08.006

牙釉质表面气相SiO₂/PDMS超疏水涂层制备及其细胞毒性与抗菌性能评价

吴一豪¹, 朱莉¹, 周鑫¹, 骆怡璇¹, 黄盛斌^1,2, 林继兴^1,2, 麻健丰^1,2*

1.温州医科大学口腔医学研究所浙江温州 325027;
2.温州医科大学口腔医学院附属口腔医院浙江温州 325007

收稿日期:2021-12-30 出版日期:2022-08-28 发布日期:2022-08-24
通讯作者: *麻健丰,E-mail:dentistmacn@aliyun.com
作者简介:吴一豪(1996~ ),男,浙江温州人,硕士在读,研究方向:口腔修复学。
基金资助:
国家自然科学基金(编号:81870813)温州市重大科技专项(编号:2018ZY012)

Preparation of Fumed Silica/polydimethylsiloxane Superhydrophobic Coating on Tooth Enamel Surface and Evaluation of Its Cytotoxicity and Antibacterial Ability

WU Yihao¹, ZHU Li¹, ZHOU Xin¹, LUO Yixuan¹, HUANG Shengbin^1,2, LIN Jixing^1,2, MA Jianfeng^1,2*

1. Institute of Stomatology, Wenzhou Medical University, Wenzhou 325027, China;
2. School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou 325007, China

Received:2021-12-30 Online:2022-08-28 Published:2022-08-24

摘要/Abstract

摘要： 目的: 在牙釉质表面制备气相二氧化硅(SiO₂)/聚二甲基硅氧烷(PDMS)超疏水涂层,并评价涂层的细胞毒性与抗菌性能。方法: 将不同质量百分比的亲/疏水SiO₂、PDMS溶于正己烷及其他相关助剂中形成悬浮溶液,喷涂于牙釉质表面形成超疏水涂层。采用接触角测量仪测量涂层的静态接触角及动态接触角;扫描电镜(SEM)观察微观结构;X射线光电子能谱(XPS)分析化学组成;傅立叶变换红外吸收光谱仪(FTIR)分析涂层内基团;MTT比色法检测细胞毒性;自动菌落形成实验(CFU)法验证其抗变形链球菌粘附能力。结果: 亲/疏水性 SiO₂质量比为1∶1时,涂层具有最好的疏水效果,其静态接触角(CA)为164°,动态接触角(SA)为1.4°;其超疏水效果来源于纳米SiO₂粒子提供的表面粗糙度和PDMS提供的低表面能;MTT 结果显示该涂层具有较好的细胞相容性;抗菌实验结果表明,超疏水涂层抗菌效果明显优于对照组,6 h以内抗粘附效果稳定,抗粘附效率达到87%以上。结论: 成功在牙釉质表面构建了生物相容性的超疏水涂层,该涂层具有较好的抗变形链球菌粘附能力,为开发口腔防龋喷剂提供思路。

关键词: 超疏水, 抗菌, 抗粘附, 变形链球菌, 聚二甲基硅氧烷

Abstract: Objective: To prepare fumed silica/polydimethylsiloxane (SiO₂/PDMS) superhydrophobic coating on the surface of tooth enamel, and evaluate the cytotoxicity and antibacterial ability of the superhydrophobic coating. Methods: Different mass percentages of hydrophilic and hydrophobic SiO₂ and PDMS were dissolved in n-hexane and other related additives to form suspension solution and sprayed on enamel surface to form superhydrophobic coating. The contact angle and sliding angle of coating were measured by contact angle measuring instrument. Scanning electron microscopy (SEM) was used to observe the microstructure. X-ray photoelectron spectroscopy (XPS) was used to analyze its element concentration. Fourier transform infrared absorption spectrometer (FTIR) was used to analyze the inner group of the coating. Cytotoxicity test was performed to detect its biocompatibility. Automatic colony forming unit (CFU) was used to verify its anti- streptococcus mutans adhesion ability. Results: The best hydrophobic effect was when the hydrophilic fumed silica: hydrophobic fumed silica was 1∶1. At this moment, the average contact angle reached 164°, and the sliding angle reached 1.4°. The superhydrophobic effect comes from the surface roughness provided by nano-SiO₂ particles and the low surface energy provided by PDMS. MTT results showed that the coating had good biocompatibility. Antibacterial test showed that the antibacterial effect of superhydrophobic coating was better than that of control group. The anti-adhesion effect was stable within 6 h, reaching more than 87%. Conclusion: A biocompatibility superhydrophobic coating was successfully constructed on the enamel surface. The coating has good adhesion resistance to Streptococcus mutans, which provides ideas for the development of oral caries prevention sprays.

Key words: superhydrophobic, antibacterial, anti adhesion, Streptococcusmutans, PDMS

吴一豪, 朱莉, 周鑫, 骆怡璇, 黄盛斌, 林继兴, 麻健丰. 牙釉质表面气相SiO₂/PDMS超疏水涂层制备及其细胞毒性与抗菌性能评价[J]. 口腔医学研究, 2022, 38(8): 725-730.

WU Yihao, ZHU Li, ZHOU Xin, LUO Yixuan, HUANG Shengbin, LIN Jixing, MA Jianfeng. Preparation of Fumed Silica/polydimethylsiloxane Superhydrophobic Coating on Tooth Enamel Surface and Evaluation of Its Cytotoxicity and Antibacterial Ability[J]. Journal of Oral Science Research, 2022, 38(8): 725-730.

参考文献

[1] Ensikat HJ, Ditsche-Kuru P, Neinhuis C, et al. Superhydrophobicity in perfection: the outstanding properties of the lotus leaf [J]. Beilstein J Nanotechnol, 2011, 2: 152-161.
[2] Shuhui L, Jianying H, Zhong C, et al. A review on special wettability textiles: theoretical models, fabrication technologies and multifunctional applications [J]. Journal of Materials Chemistry A, 2017, 5(1):31-55.
[3] Thomas Y. An essay on the cohesion of fluids [J]. Philos Trans R Soc Lond B Biol Sci, 1805, 95: 65-87.
[4] Wenzel RN. Resistance of solid surfaces to wetting by water [J]. Industrial Engineering Chemistry, 2002, 28(8):988-994.
[5] Cassie ABD. Wettability of porous surfaces [J]. Transactions of the Faraday Society, 1944, 40(5):546-551.
[6] Simpson JT, Hunter SR, Aytug T. Superhydrophobic materials and coatings: a review [J]. Rep Prog Phys, 2015, 78(8):086501.
[7] Feng L, Zhang Z, Mai Z, et al. A super-hydrophobic and super-oleophilic coating mesh film for the separation of oil and water [J]. Angew Chem Int Ed Engl, 2004, 43(15):2012-2014.
[8] Neinhuis C, Barthlott W. Characterization and distribution of water-repellent, self-cleaning plant surfaces [J]. Ann Bot, 1997, 79(6): 667-677.
[9] Wang S, Liu K, Yao X, et al. Bioinspired surfaces with superwettability: New insight on theory, design, and applications [J]. Chem Rev, 2015, 115(16):8230-8293.
[10] Nakajima A, Fujishima A, Hashimoto K, et al. Preparation of transparent superhydrophobic boehmite and silica films by sublimation of aluminum acetylacetonate [J]. Adv Mater, 1999, 11(16): 1365-1368.
[11] Deng X, Mammen L, Butt HJ, et al. Candle soot as a template for a transparent robust superamphiphobic coating [J]. Science, 2012, 335(6064):67-70.
[12] Cao M, Li K, Dong Z, et al. Superhydrophobic “Pump”: Continuous and spontaneous antigravity water delivery [J]. Adv Funct Mater, 2015, 25(26): 4114-4119.
[13] Katsikogianni M, Missirlis YF. Concise review of mechanisms of bacterial adhesion to biomaterials and of techniques used in estimating bacteria-material interactions [J]. Eur Cell Mater, 2004, 8:37-57.
[14] Gong X, He S. Highly durable superhydrophobic polydimethylsiloxane/silica nanocomposite surfaces with good self-cleaning ability [J]. ACS Omega, 2020, 5(8):4100-4108.
[15] Zihui L, Min G, Binghai D, et al. Transparent and robust SiO₂/PDMS composite coatings with self-cleaning [J]. Surface Engineering, 2020, 36:643-650.
[16] Xie H, Hao, Zhang YY, et al. Anti-Icing performance of a coating based on nano/microsilica particle-filled amino-terminated PDMS-modified epoxy [J]. Coatings, 2019, 9(12):771.
[17] O'toole G, Kaplan HB, Kolter R. Biofilm formationas microbial development [J]. Annu Rev Microbiol, 2000, 54(1):49-79.
[18] Dewhirst FE, Chen T, Izard J, et al. The human oral microbiome [J]. J Bacteriol, 2010, 192(19): 5002-5017.
[19] Bayer IS. Superhydrophobic coatings from ecofriendly materials and processes: a review [J]. Adv Mater Interfaces, 2020, 7(13): 2000095.
[20] Ding Q, Xu X, Yue Y, et al. Nanocellulose-mediated electroconductive self-healing hydrogels with high strength, plasticity, viscoelasticity, stretchability, and biocompatibility toward multifunctional applications[J]. ACS Appl Mater Interfaces, 2018, 10(33):27987-28002.
[21] Tian P, Guo Z. Bioinspired silica-based superhydrophobic materials [J]. Applied Surface Science, 2017, 426:1-18.
[22] Gao S, Dong X, Huang J, et al. Rational construction of highly transparent superhydrophobic coatings based on a non-particle, fluorine-free and water-rich system for versatile oil-water separation [J]. Chemical Engineering Journal, 2018, 333:621-629.
[23] Kawai K, Urano M, Ebisu S. Effect of surface roughness of porcelain on adhesion of bacteria and their synthesizing glucans [J]. J Prosthet Dent, 2000, 83(6):664-667.
[24] Wu X, Fu Q, Kumar D, et al. Mechanically robust superhydrophobic and superoleophobic coatings derived by sol-gel method [J]. Materials & Design,2016,89:13021309.
[25] Tu K, Wang X, Kong L, et al. Facile preparation of mechanically durable, self-healing and multifunctional superhydrophobic surfaces on solid wood [J]. Materials & Design,2018,140:30-36.
[26] Yue Y, Hays MP, Hardwidge PR, et al.Surface characteristics influencing bacterial adhesion to polymeric substrates [J]. RSC Advances, 2017, 7:14254.

牙釉质表面气相SiO₂/PDMS超疏水涂层制备及其细胞毒性与抗菌性能评价

Preparation of Fumed Silica/polydimethylsiloxane Superhydrophobic Coating on Tooth Enamel Surface and Evaluation of Its Cytotoxicity and Antibacterial Ability

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	封雪, 程磊. 季铵盐DMADDM抗菌改性牙科材料的研究进展[J]. 口腔医学研究, 2022, 38(5): 396-399.
[2]	徐小雨, 郭梦芹, 余海婧, 刘洋洋, 郭鹏. 杨梅黄酮对变形链球菌毒力因子及生物膜的抑制作用研究[J]. 口腔医学研究, 2022, 38(4): 344-349.
[3]	许来俊, 袁鹤, 李继遥. 负载氯己定的可注射水凝胶对粪肠球菌及生物膜的体外抑菌效果[J]. 口腔医学研究, 2022, 38(1): 45-50.
[4]	全璐璐, 李雪微, 赵俊杰, 邓文振, 张旭, 董伟, 梁永强. 正畸改性流动树脂的体外研究[J]. 口腔医学研究, 2021, 37(8): 712-716.
[5]	徐仰龙. 缓释型柠檬苦素纳米聚合物抗生物膜作用的研究[J]. 口腔医学研究, 2021, 37(5): 407-411.
[6]	叶伟龙, 郑靖川, 黄佳倩, 赵鹏程, 刘柱, 马国武. 分级电纺复合载药微球辅助牙槽骨增量[J]. 口腔医学研究, 2021, 37(5): 458-461.
[7]	杨柳青, 王博灏, 谢立莉. EGCG改性牙科粘接剂的抗菌研究[J]. 口腔医学研究, 2021, 37(2): 131-134.
[8]	麻健丰, 金小婷. 超疏水材料在口腔医学中的应用[J]. 口腔医学研究, 2020, 36(9): 803-807.
[9]	苗强, 郑亚飞, 夏雨凝, 张薇, 王菁, 马赛, 马楚凡. 二氧化钛纳米管/抗菌肽控释系统的构建及初步性能研究[J]. 口腔医学研究, 2020, 36(9): 871-874.
[10]	赵霞, 徐嘉昊, 夏商, 张晨, 王万春. Farnesol纳米胶束的构建及对变形链球菌的抑菌作用研究[J]. 口腔医学研究, 2020, 36(9): 875-878.
[11]	裴喜燕, 孙凤. 微创牙周非手术治疗研究进展[J]. 口腔医学研究, 2020, 36(7): 622-625.
[12]	金小婷, 史诗, 陈欢欢, 薛冬, 麻健丰. 釉质表面超疏水凝胶纳米涂层的制备及其性能研究[J]. 口腔医学研究, 2020, 36(6): 585-590.
[13]	王楠, 周延民. 石墨烯材料在口腔医学领域的应用及生物安全性研究[J]. 口腔医学研究, 2020, 36(5): 410-412.
[14]	许志强, 曾秀峰, 洪少楠, 曾秀霞, 贺于奇, 黄俊徽. 不同管径二氧化钛纳米管的抗菌性能研究[J]. 口腔医学研究, 2020, 36(3): 230-234.
[15]	杨成雪, 翟羽翔, 王开梅, 陈林. 纳米无机载银磷酸锆树脂基托对6种口腔细菌的体外抗菌研究[J]. 口腔医学研究, 2020, 36(3): 243-246.