EGCG改性牙科粘接剂的抗菌研究

doi:10.13701/j.cnki.kqyxyj.2021.02.009

摘要/Abstract

摘要： 目的:评估添加不同浓度表没食子儿茶素没食子酸酯(EGCG)对正畸粘接剂抗菌性能和粘接性能的影响,为正畸临床预防龋白斑提供依据。方法:制备含有0(对照)、0.1、1.0和10 g/L EGCG的实验粘接剂。通过活死菌染色和MTT评估实验粘接剂抗菌性能。通过剪切强度和聚合度测试评估EGCG对粘接性能的影响。结果:与对照组相比,添加不同浓度EGCG的粘接剂均具有显著抗菌作用(P<0.05),且随EGCG浓度增加而效果显著提升(P<0.05)。添加10 g/L EGCG显著降低粘接剂剪切强度和聚合度(P<0.05),而0.1和1.0 g/L EGCG组则无影响(P>0.05)。结论:添加1.0 g/L EGCG可以在不干扰粘接剂粘接性能的前提下,发挥良好的抗菌作用。

关键词: 表没食子儿茶素没食子酸酯, 龋白斑, 粘接剂, 抗菌

Abstract: Objective: To evaluate the antibacterial and bonding performance of adhesives incorporated with different concentrations of epigallocatechin-3-gallate (EGCG), and provide experimental support for the usage in preventing the occurrence of white spot lesions in orthodontic treatment. Methods: Four experimental groups were designed, namely 0 (Control group), 0.1, 1.0, and 10 g/L EGCG groups. The antibacterial ability of experimental adhesives was assessed by live/dead bacterial staining and MTT assay. The shear bond strength (SBS) and degree of conversion (DC) were evaluated to assess the bonding performance of experimental adhesives. Results: Comparing with the Control group, the incorporation of EGCG into adhesive significantly increased the antibacterial ability (P<0.05), which had positive correlation with EGCG concentration. No negative influence on the SBS and DC was detected for 0.1 and 1.0 g/L EGCG groups (P>0.05), but the value of 10 g/L EGCG group significantly decreased (P<0.05). Conclusion: The addition of 1.0 g/L EGCG into dental adhesive could exert positive antibacterial ability without hampering the polymerization of dental adhesive.

Key words: epigallocatechin-3-gallate, white spot lesion, adhesive, antibacterial

杨柳青, 王博灏, 谢立莉. EGCG改性牙科粘接剂的抗菌研究[J]. 口腔医学研究, 2021, 37(2): 131-134.

YANG Liuqing, Wang Bohao, XIE Lili. Evaluation of Antibacterial Ability of Epigallocatechin-3-gallate-modified Adhesive[J]. Journal of Oral Science Research, 2021, 37(2): 131-134.

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