负载氯己定的可注射水凝胶对粪肠球菌及生物膜的体外抑菌效果

doi:10.13701/j.cnki.kqyxyj.2022.01.010

口腔医学研究 ›› 2022, Vol. 38 ›› Issue (1): 45-50.DOI: 10.13701/j.cnki.kqyxyj.2022.01.010

负载氯己定的可注射水凝胶对粪肠球菌及生物膜的体外抑菌效果

许来俊^1,2, 袁鹤¹, 李继遥^1*

1.口腔疾病研究国家重点实验室国家口腔疾病临床医学研究中心四川大学华西口腔医院牙体牙髓病科四川成都 610041;
2.中南大学湘雅口腔医学院湘雅口腔医院牙体牙髓病科口腔健康研究湖南省重点实验室湖南省口腔重大疾病与口腔健康临床医学研究中心湖南长沙 410008

收稿日期:2021-08-23 出版日期:2022-01-28 发布日期:2022-01-21
通讯作者: * 李继遥,E-mail: jiyaoliscu@163.com
作者简介:许来俊(1990~ ),男,湖南常德人,博士,主治医师,研究方向:成骨材料的研究。
基金资助:
国家自然科学基金(编号:81701028);湖南省卫生健康委员会科研基金(编号:202208015033)

Antibacterial Effect of Injectable Hydrogel with Chlorhexidine on Enterococcus Faecalis and Biofilm in Vitro

XU Laijun^1,2, YUAN He¹, LI Jiyao^1*

1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China;
2. Xiangya School of Stomatology & Xiangya Stomatological Hospital, Central South University & Hunan Key Laboratory of Oral Health Research, Hunan Clinical Research Center of Oral Major Diseases and Oral Health, Changsha 410008, China

Received:2021-08-23 Online:2022-01-28 Published:2022-01-21

摘要/Abstract

摘要： 目的:研究负载氯己定的结冷胶复合材料体外抗粪肠球菌及生物膜的效果。方法:通过添加钙离子交联剂,制备含有纳米羟基磷灰石的结冷胶复合材料,并有效负载氯己定。通过对细菌生长曲线测定、扫描电镜等评价水凝胶体外抗粪肠球菌的效果,结晶紫染色定量和共聚焦激光扫描显微镜观察水凝胶对生物膜的作用。结果:随着水凝胶中氯己定浓度的增加,抗菌活性逐渐增强。当氯己定浓度为50 μg/mL时,可显著抑制细菌生长及生物膜形成;当浓度为250 μg/mL时,可表现出显著杀菌性。结论:负载氯己定的结冷胶复合材料抑制粪肠球菌生长及清除生物膜作用明显,有望作为新型可注射的根管消毒材料,促进顽固性根尖周炎的愈合。

关键词: 结冷胶, 水凝胶, 粪肠球菌, 抗菌性, 生物膜

Abstract: Objective: To study the effect of chlorhexidine-loaded gellan gum hydrogel against Enterococcus faecalis and biofilm in vitro. Methods: The gellan gum containing hydroxyapatite was prepared by adding crosslinking agent (calcium ion), and chlorhexidine was effectively loaded. The effect of hydrogels against Enterococcus faecalis in vitro was evaluated by measuring bacterial growth curves and scanning electron microscopy, and their inhibiting the bacterial biofilms was observed by quantitative crystal violet staining and confocal laser scanning microscope. Results: As the concentration of chlorhexidine in the hydrogel increased, the antibacterial activity gradually increased. When the concentration of chlorhexidine was 50 μg/mL, it could significantly inhibit bacterial growth and biofilm formation. When the concentration was 250 μg/mL, it could show significant bactericidal effect. Conclusion: The gellan gum hydrogel loaded with chlorhexidine presented great effects on inhibiting the growth of Enterococcus faecalis and removing their biofilm. It might be a novel injectable biomaterial to disinfect root canals and promote the healing of refractory periapical periodontitis.

Key words: gellan gum, hydrogel, Enterococcus faecalis, antibacterial, biofilm

许来俊, 袁鹤, 李继遥. 负载氯己定的可注射水凝胶对粪肠球菌及生物膜的体外抑菌效果[J]. 口腔医学研究, 2022, 38(1): 45-50.

XU Laijun, YUAN He, LI Jiyao. Antibacterial Effect of Injectable Hydrogel with Chlorhexidine on Enterococcus Faecalis and Biofilm in Vitro[J]. Journal of Oral Science Research, 2022, 38(1): 45-50.

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负载氯己定的可注射水凝胶对粪肠球菌及生物膜的体外抑菌效果

Antibacterial Effect of Injectable Hydrogel with Chlorhexidine on Enterococcus Faecalis and Biofilm in Vitro

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