分级电纺复合载药微球辅助牙槽骨增量

doi:10.13701/j.cnki.kqyxyj.2021.05.016

口腔医学研究 ›› 2021, Vol. 37 ›› Issue (5): 458-461.DOI: 10.13701/j.cnki.kqyxyj.2021.05.016

• 口腔干细胞与再生研究 • 上一篇下一篇

分级电纺复合载药微球辅助牙槽骨增量

叶伟龙^1,2, 郑靖川^1,2, 黄佳倩^1,2, 赵鹏程^1,2, 刘柱^1,2, 马国武^1,2*

1.大连医科大学口腔医学院辽宁大连 116044;
2.大连医科大学口腔医学院附属口腔医院辽宁大连 116023

收稿日期:2020-10-09 发布日期:2021-05-17
通讯作者: * 马国武,E-mail:mgw640242000@aliyun.com
作者简介:叶伟龙(1995~ ),江苏连云港人,硕士在读,主要从事口腔颌面组织工程研究。
基金资助:
国家自然科学基金面上项目(编号:81671827)

Drugs Loaded Hierarchically Electrospraying Composite Microspheres Support Dental Alveolar Bone Augmentation

YE Weilong^1,2, ZHENG Jingchuan^1,2, HUANG Jiaqian^1,2, ZHAO Pengcheng^1,2, LIU Zhu^1,2, MA Guowu^1,2*

1. School of Stomatology, Dalian Medical University, Dalian 116044, China;
2. Affiliated Stomatological Hospital of School of Stomatology, Dalian Medical University, Dalian 116023, China

Received:2020-10-09 Published:2021-05-17

摘要/Abstract

摘要： 目的:分级电纺法制备PLGArhBMP2@CSPac525复合微球,研究其抗菌与成骨活性。方法:分级电纺法制备双载药PLGA@CS复合微球,扫描电镜检测微观结构,紫外分光光度计和Elisa检测包封效率。抑菌环试验与碱性磷酸酶染色测试抗菌与成骨的活性。结果:复合微球呈理想的球包球结构,粒径均一约280~290 μm,rhBMP与Pac525包封率均大于90%。在30 d时释放的Pac525仍可形成超过10 mm的抑菌圈, 碱性磷酸酶染色证实第20、40、60天时释放的rhBMP2在诱导第7天时即有明显的成骨表现。结论:本研究为口腔种植前骨增量治疗提出了一种可靠而高效的生物多功能支架方案。

关键词: 分级电纺, 复合微球, 抗菌, 成骨

Abstract: Objective: To construct PLGArhBMP2@CSPac525 composite microspheres by electrospraying and study their anti-bacteria and osteogenic effects in dental alveolar bone augmentation. Methods: PLGArhBMP2@CSPac525 composite microspheres were fabricated by hierarchically electrospraying. SEM, UV-Vis, and ELISA examination were performed to test the morphology and encapsulation efficiency of composite microspheres. Inhibitor ring test and alkaline phosphatase staining were used to verify the anti-bacteria and osteogenic bioactivity of Pac525 and rhBMP2 loaded in microspheres. Results: The composite microspheres demonstrated ideal sphere-in-sphere structure. The encapsulation efficiencies of Pac525 and rhBMP2 were both more than 90%. Diameter of inhibition ring was more than 10 mm with elution at 30th day, and significant alkaline phosphatase staining were found after osteogenic inducing with elusion for 7 days. Conclusion: These hierarchically microspheres might be serve as a promising, reliable and efficient multi-function bioactive scaffold for pre-implantation bone augmentation

Key words: hierarchically electrospraying, composite microsphere, anti-bacteria, osteogenic

叶伟龙, 郑靖川, 黄佳倩, 赵鹏程, 刘柱, 马国武. 分级电纺复合载药微球辅助牙槽骨增量[J]. 口腔医学研究, 2021, 37(5): 458-461.

YE Weilong, ZHENG Jingchuan, HUANG Jiaqian, ZHAO Pengcheng, LIU Zhu, MA Guowu. Drugs Loaded Hierarchically Electrospraying Composite Microspheres Support Dental Alveolar Bone Augmentation[J]. Journal of Oral Science Research, 2021, 37(5): 458-461.

参考文献

[1] Schmitz JP, Hollinger JO. The critical size defect as an experimental model for craniomandibulofacial nonunions [J]. Clin Orthop Relat Res, 1986, (205):299-308.
[2] Seitz O, Dehner J, Schürmann C, et al. Periimplantitis [J]. Der Mkg Chirurg, 2011, 4(4):295-300.
[3] Urist M. Bone: formation by autoinduction [J]. Science, 1965, 150(3698):893-899.
[4] 唐六一. 骨形态发生收蛋白的载体及其释放系统[J].中华骨科杂志, 1993, 13(2):140-142.
[5] Wei SY, Wu JM, Kuo YY, et al. Solution structure of a novel tryptophan-rich peptide with bidirectional antimicrobial activity [J]. J Bacteriol, 2006, 188(1):328-334.
[6] Li JY, Wang XJ, Wang LN, et al. High in vitro antibacterial activity of Pac-525 against Porphyromonas gingivalis biofilms cultured on titanium[J]. Biomed Res Int, 2015, 2015:909870.
[7] Yamamoto M, Takahashi Y, Tabata Y. Controlled release by biodegradable hydrogels enhances the ectopic bone formation of bone morphogenetic protein [J]. Biomaterials, 2003, 24(24):4375-4383.
[8] 王健群,张斌.聚乳酸-羟基乙酸共聚物微球在骨组织工程中的应用[J].口腔医学研究,2020,36(9):817-820.
[9] Wang M, Feng Q, Niu X, et al. A spheres-in-sphere structure for improving protein-loading poly (lactide-co-glycolide) microspheres [J]. Polym Degrad Stabil, 2010, 95(1): 6-13.
[10] He Y, Jin Y, Wang X, et al. An antimicrobial peptide-loaded gelatin/chitosan nanofibrous membrane fabricated by sequential layer-by-layer electrospinning and electrospraying techniques [J]. Nanomaterials, 2018, 8(5): 327.
[11] Liu Z, Ye W, Zheng J, et al. Hierarchically electrospraying a PLGA@chitosan sphere-in-sphere composite microsphere for multi-drug-controlled release [J]. Regen Biomater,2020,7(4):381-390.
[12] Li Y, Na R, Wang X, et al. Fabrication of antimicrobial peptide-loaded PLGA/chitosan composite microspheres for long-acting bacterial resistance [J]. Molecules, 2017, 22(10):1637.

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分级电纺复合载药微球辅助牙槽骨增量

Drugs Loaded Hierarchically Electrospraying Composite Microspheres Support Dental Alveolar Bone Augmentation

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