UBE2T通过激活AKT信号通路促进口腔鳞状细胞癌细胞增殖

doi:10.13701/j.cnki.kqyxyj.2026.10.009

摘要/Abstract

摘要： 目的: 探讨泛素结合酶E2T(ubiquitin-conjugating enzyme E2T,UBE2T)在口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)组织和细胞中的表达及对OSCC细胞增殖、侵袭、迁移等生物学行为的影响及相关机制。方法: 使用TIMER数据库和GEPIA数据库分析头颈部鳞状细胞癌(head and neck squamous cell carcinoma,HNSC)组织中UBE2T-mRMA表达水平。使用免疫组织化学法验证OSCC组织中UBE2T的表达,验证UBE2T的表达与OSCC患者临床病理特征和预后的关系。通过Western blot和逆转录定量聚合酶链反应验证UBE2T在OSCC细胞系中的表达水平。转染UBE2T干扰mRNA后,通过体外细胞实验验证UBE2T对OSCC细胞CAL-27的生物行为功能的影响并检测干扰后的蛋白激酶B(protein kinase B,AKT)信号通路的表达。结果: 与癌旁及正常组织相比较,UBE2T在OSCC中表达显著升高(P＜0.05),高表达UBE2T组与低表达组相比总体生存时间明显缩短(P＜0.05),OSCC的分化程度与UBE2T的表达呈负相关(P＜0.05)。转染si-UBE2T后的CAL-27细胞与si-NC组相比较,si-UBE2T组在增殖、迁移和侵袭能力均低于si-NC组(P＜0.05)。si-UBE2T组与si-NC组相比,si-UBE2T组中磷酸化蛋白激酶B(phosphorylated protein kinase B,p-AKT)蛋白表达显著降低(P＜0.05)。结论: UBE2T在OSCC组织中高表达且患者生存时间明显缩短。干扰UBE2T表达后OSCC细胞的增殖、侵袭、迁移能力降低并可能通过调控磷酸化AKT信号通路调节OSCC细胞的增殖。

关键词: 口腔鳞状细胞癌, 泛素结合酶E2T, 蛋白激酶B, 细胞迁移, 细胞增殖

Abstract: Objective: To investigate the expression of ubiquitin-conjugating enzyme E2T (UBE2T) in oral squamous cell carcinoma tissues and cells, as well as its effects on the biological behaviors of oral squamous cell carcinoma (OSCC) cells including proliferation, invasion, and migration, along with the related mechanisms. Methods: The TIMER database and GEPIA database were used to analyze the expression level of UBE2T-mRNA in head and neck squamous cell carcinoma tissues. Immunohistochemistry was employed to validate UBE2T expression in OSCC tissues. Statistical methods were used to assess the relationship between UBE2T expression and the clinical pathological characteristics and prognosis of OSCC patients. Western blotting and real-time quantitative PCR were utilized to validate UBE2T expression levels in OSCC cell lines. After transfection with UBE2T interfering mRNA, the effect of UBE2T on the biological behavior of the OSCC cell line CAL-27 through in vitro cell experiments was validated and the expression of the protein kinase B (AKT) signaling pathway after interference was detected. Results: Compared with adjacent and normal tissues, UBE2T expression was significantly elevated in OSCC (P<0.05). The overall survival time in the high UBE2T expression group was markedly shorter than that in the low expression group (P<0.05). The differentiation degree of OSCC showed a negative correlation with UBE2T expression (P<0.05). Compared with the si-NC group, CAL-27 cells transfected with si-UBE2T exhibited significantly lower proliferation, migration, and invasion capacities (P<0.05). Compared with the si-NC group, the si-UBE2T group showed significantly reduced levels of phosphorylated protein kinase B protein (P<0.05). Conclusion: UBE2T is highly expressed in OSCC tissues and associated with significantly shorter patient survival. Silencing UBE2T expression reduced the proliferation, invasion, and migration abilities of OSCC cells, potentially through regulating the phosphorylated AKT signaling pathway to modulate OSCC cell proliferation.

Key words: oral squamous cell carcinoma, ubiquitin-conjugating enzyme E2T, protein kinase B, cell migration, cell proliferation

杨森, 王晖予, 张佳佳, 方桃莉, 徐江. UBE2T通过激活AKT信号通路促进口腔鳞状细胞癌细胞增殖[J]. 口腔医学研究, 2026, 42(10): 877-883.

YANG Sen, WANG Huiyu, ZHANG Jiajia, FANG Taoli, XU Jiang. UBE2T Promotes Proliferation of Oral Squamous Cell Carcinoma by Activating AKT Signaling Pathway[J]. Journal of Oral Science Research, 2026, 42(10): 877-883.

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