透明质酸对IL-1β刺激下髁突软骨细胞中HMGB-1表达的影响

doi:10.13701/j.cnki.kqyxyj.2018.03.007

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (3): 241-244.DOI: 10.13701/j.cnki.kqyxyj.2018.03.007

透明质酸对IL-1β刺激下髁突软骨细胞中HMGB-1表达的影响

李程^1,2, 邓末宏^1,3, 郭慧琳^1,3, 冯亚平¹, 房维^1,3*, 龙星^1,3*

1. 武汉大学口腔医学院口腔基础医学省部共建国家重点实验室培训基地和口腔生物医学教育部重点实验室湖北武汉 430079;
2. 武汉大学口腔医院青山门诊湖北武汉 430080;
3. 武汉大学口腔医院第二颌面外科湖北武汉 430079

收稿日期:2018-01-29 出版日期:2018-03-30 发布日期:2018-03-27
通讯作者: *房维,E-mail:fang.wei@whu.edu.cn;龙星,E-mail:longxing@whu.edu.cn
作者简介:李程(1988~ ),女,湖北宜昌人,博士,主治医师,主要从事颞下颌关节疾病相关研究。
基金资助:
国家自然科学基金(编号:81470761、81671013、81771100);武汉大学青年教师资助项目 (编号:2042017kf0140)

Influence of Hyaluronic Acid on HMGB-1 Expression of Chondrocytes with IL-1β Stimulation.

LI Cheng^1,2, DENG Mo-hong^1,3, GUO Hui-lin^1,3, FENG Ya-ping¹, FANG Wei^1,3*, LONG Xing^1,3*

1. The State Key Laboratory Breeding Base of Basic Science of Stomatology and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China;
2. Qingshan Clinic, School and Hospital of Stomatology, Wuhan University, Wuhan 430080, China;
3. Department of 2nd Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China

Received:2018-01-29 Online:2018-03-30 Published:2018-03-27

摘要/Abstract

摘要： 目的: 研究透明质酸对IL-1β刺激下髁突软骨细胞中HMGB-1表达的影响。方法: 体外分离培养大鼠髁突软骨细胞,将透明质酸(hyaluronic acid,HA)和甘草酸(glycyrrhizin,GL)分别与IL-1β同时加入髁突软骨细胞中,刺激24 h后提取各组细胞总RNA,检测HMGB-1及其相关受体基因表达;提取细胞总蛋白检测HMGB-1蛋白表达。结果: IL-1β刺激髁突软骨细胞24 h后 HMGB-1、TLR2和TLR4基因表达显著升高(P<0.05);IL-1β与HA或GL同时加入刺激的软骨细胞与仅加入IL-1β刺激组相比,HMGB-1、TLR2和TLR4的基因表达较的表达显著降低,HMGB-1蛋白表达也显著降低(P<0.05)。结论: HA可降低IL-1β刺激后髁突软骨细胞中HMGB-1及其受体TLR2、TLR4的表达。

关键词: 髁突, 软骨细胞, 透明质酸, HMGB-1, IL-1β

Abstract: Objective: To study the effect of hyaluronic acid (HA) on HMGB-1 expression of chondrocytes with IL-1β stimulation. Methods: TMJ condylar chondrocytes were obtained through in vitro culture. HA and glycyrrhizin (GL) were respectively incubated in chondrocytes with IL-1β stimulation. Total RNA and protein were extracted 24h after stimulation to detect the mRNA expression of HMGB-1 and receptors, and protein expression of HMGB-1. Results: After IL-1β stimulation, the mRNA expressions of HMGB-1, TLR2, and TLR4 were increased. The mRNA expressions of HMGB-1, TLR2, and TLR4 were significantly decreased with the addition of HA or GL in contrast to only IL-1β stimulation (P<0.05). Also the protein expression of HMGB-1 was decreased after HA or GL stimulation (P<0.05). Conclusion: HA could decrease the HMGB-1 and receptors expression of chondrocytes in IL-1β stimulation.

Key words: Condyle, Chondrocyte, Hyaluronic acid, HMGB-1, IL-1β

李程, 邓末宏, 郭慧琳, 冯亚平, 房维, 龙星. 透明质酸对IL-1β刺激下髁突软骨细胞中HMGB-1表达的影响[J]. 口腔医学研究, 2018, 34(3): 241-244.

LI Cheng, DENG Mo-hong, GUO Hui-lin, FENG Ya-ping, FANG Wei, LONG Xing. Influence of Hyaluronic Acid on HMGB-1 Expression of Chondrocytes with IL-1β Stimulation.[J]. Journal of Oral Science Research, 2018, 34(3): 241-244.

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透明质酸对IL-1β刺激下髁突软骨细胞中HMGB-1表达的影响

Influence of Hyaluronic Acid on HMGB-1 Expression of Chondrocytes with IL-1β Stimulation.

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