口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (4): 414-418.

• 口腔颌面外科学研究 • 上一篇    下一篇

DEK基因的小干扰RNA转染对舌鳞状细胞癌细胞增殖和凋亡的影响

冯铁军1*, 王玉栋1, 潘宣1, 池宇峰2   

  1. 1. 广东药科大学附属第一医院口腔科 广东 广州 510080;
    2. 韶关市第一人民医院口腔科 广东 韶关 512000
  • 收稿日期:2017-08-26 出版日期:2018-04-28 发布日期:2018-04-25
  • 通讯作者: 冯铁军,E-mail:fengtiejun1967@163.com
  • 作者简介:冯铁军(1967~ ),男,广东广州人,学士,副主任医师,主要从事口腔科临床修复学研究。
  • 基金资助:
    广东省自然科学基金(编号:S2013010015952)

Effect of Transfection of DEK Gene Small Interference RNA on Proliferation and Apoptosis of Tongue Squamous Cell Carcinoma.

FENG Tie-jun1, WANG Yu-dong1, PAN Xuan1, CHI Yu-feng2   

  1. 1. Department of Stomatology, the First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510080, China;
    2. Department of Stomatology, the First People's Hospital of Shaoguan, Shaoguan 512000, China.
  • Received:2017-08-26 Online:2018-04-28 Published:2018-04-25

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摘要： 目的:RNA干扰DEK基因表达对舌鳞状细胞癌(简称鳞癌)细胞增殖凋亡的影响。方法:采用实时荧光定量聚合酶链反应(PCR)检测舌鳞癌组织中DEK基因的表达;体外培养人舌鳞癌细胞系Tca8113和CAL-27,将合成的阴性对照小干扰RNA(siRNA,阴性对照组)及DEK-siRNA(转染组)转染至细胞,不经特殊处理的细胞为空白对照组,分别在转染48 h后,用细胞计数试剂盒(CCK-8)法、流式细胞术及Western blot等方法检测DEK对Tca8113和CAL-27细胞增殖、凋亡及DEK、B细胞淋巴瘤/白血病-2(bcl-2)、B细胞淋巴瘤/白血病-2相关X蛋白(bax)、磷脂酰肌醇-3激酶(PI3K)、磷酸化蛋白激酶B(p-Akt)蛋白表达影响。结果:舌鳞癌组织中DEK基因明显升高,与癌旁组织比较差异有统计学意义(P<0.05);与空白对照组比较,DEK-siRNA转染Tca8113和CAL-27后DEK的表达水平明显降低,细胞增殖活力降低,凋亡率增加,Bcl-2、PI3K、p-Akt蛋白表达均显著下调,bax蛋白表达显著上调(P<0.05)。结论:抑制DEK基因表达可通过PI3K/Akt信号通路降低舌鳞癌细胞增殖,诱导细胞凋亡。

关键词: DEK基因, 舌鳞状细胞癌, 增殖, 凋亡, 信号通路

Abstract: Objective: To study the effect of inhibiting of DEK gene expression by RNA interference on proliferation and apoptosis of tongue squamous cell carcinoma. Methods: The expression of DEK gene in tongue squamous cell carcinoma was detected by real-time PCR. Cell line Tca8113 and CAL-27 were cultured in vitro. Negative control siRNA (negative control group) and DEK siRNA (transfection group) were transfected into cells, and blank control group was not special treated. After transfected for 48h, the proliferation, apoptosis and DEK, Bcl-2, Bax, PI3K, and p-Akt protein expression on Tca8113 and CAL-27 cells were detected by CCK8, flow cytometry, and Western blot. Results: The expression of DEK gene in squamous cell carcinoma of tongue was significantly higher than that in adjacent tissues (P<0.05). Compared with the blank control group, the expression of DEK significantly decreased after DEK-siRNA was transfected into Tca8113 and CAL-27 cells. Furthermore, cell proliferation activity was significantly decreased, apoptosis rate was significantly increased, the expressions of Bcl-2, PI3K, and p-Akt protein were significantly down regulated, and the expression of Bax protein was significantly increased (P<0.05). Conclusion: The inhibition of DEK gene expression can reduce the proliferation of tongue squamous cell carcinoma cells and induced apoptosis through PI3K/Akt signaling pathway.

Key words: DEK gene, Tongue squamous cell carcinoma, Proliferation, Apoptosis, Signaling pathway