口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (4): 442-447.DOI: 10.13701/j.cnki.kqyxyj.2018.04.024

• 其他研究 • 上一篇    下一篇

三七总皂苷在兔牙齿移动过程中对牙周组织中破骨细胞及BMP-2表达的影响

蒋星宇1,2, 张玉峰1,2, 杨璐1, 孙晋虎1, 黎婷1,2*   

  1. 1. 徐州医科大学口腔医学院 江苏 徐州 221000;
    2. 徐州医科大学附属第二医院口腔科 江苏 徐州 221000
  • 收稿日期:2017-11-03 出版日期:2018-04-28 发布日期:2018-04-25
  • 通讯作者: 黎婷,E-mail:2492767182@qq.com
  • 作者简介:蒋星宇(1990~ ),男,安徽金寨人,硕士,医师,主 要从事口腔科的临床治疗工作。
  • 基金资助:
    徐州市科技计划项目(编号:KC14SH089、 KH17037)

Effects of Panaxnotoginseng Saponins on Osteoclasts and Expression of BMP-2 in Periodontal Tissues of Rabbits during Teeth Movement.

JIANG Xing-yu1,2, ZHANG Yu-feng1,2, YANG Lu1, SUN Jin-hu1, LI Ting1,2.   

  1. 1. School of Stomatology, Xuzhou Medical University, Xuzhou 221000, China;
    2. Department of Stomatology, The Affiliated Second Hospital, Xuzhou Medical University, Xuzhou 221000, China.
  • Received:2017-11-03 Online:2018-04-28 Published:2018-04-25

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摘要： 目的:通过建立兔子牙移动的模型,研究三七总皂苷(panax notoginseng saponin,PNS)对正牙过程中牙槽骨改建及牙周组织中BMP-2表达的影响。方法:选取32只3~6月龄的新西兰白兔随机分成PNS组和生理盐水(normal saline,NS)组,通过肌肉注射血栓通[40 mg/kg·d-1]和NS。每组各16只,又分为7、14、21、28 d 4个亚组,分别于正畸牙移动1周、2周、3周、4周4个时间点分别处死各组动物取材,使用苏木精－伊红(HE)染色观察牙周膜及牙槽骨的变化,抗酒石酸酸性磷酸酶(TRAP)染色检测破骨细胞,免疫组织化学染色(immunohistochemical,IHC)检测骨形态发生蛋白-2(bone morphogenetic protein-2,BMP-2)的表达变化。结果:PNS组破骨细胞数目在第1周达到高峰,显著高于NS组(P＜0.05),然后持续下降,至第3周时下降至NS组水平,无统计学差别。从第1周开始,PNS组BMP-2的表达高于NS组(P＜0.05),在第2周达到高峰,显著高于对照组(P＜0.05),然后持续下降,第3周、第4周时PNS组与NS组相比无显著差异。结论:三七总皂苷能在给药初始时期使牙周组织中破骨细胞的数量得到提升,还能提高牙周组织中BMP-2抗体蛋白的表达量,提示PNS在正畸牙齿移动的骨组织改建过程中具有积极作用。

关键词: 三七总皂苷, 骨形态发生蛋白-2, 牙齿移动, 破骨细胞

Abstract: Objectives: To study the effect of panaxnotoginseng saponins (PNS) on the alveolar bone remodeling and the expression of BMP-2 in rabbit periodontal tissues during orthodontic teeth movement. Methods: Thirty-two 3-6-month-old male New Zealand white rabbits were casually divided into two groups: PNS group and control group, which were injected with xueshaungtong and normal saline by intramuscular injection. Each group was divided into 7, 14, 21, and 28 days four subgroups, respectively. The rabbits in each group were sacrificed to obtain mandibular tooth specimens after 1, 2, 3, and 4 weeks. The changes of periodontal ligament and alveolar bone were observed by hematoxylin-eosin (HE) staining. Tartrate-resistant acid phosphatase (TRAP) staining was used to detect osteoclasts numbers. Immunohistochemical (IHC) staining was used to detect the expression of BMP-2 in periodontal tissues of the teeth. Results: The numbers of osteoclasts in the PNS group reached a peak at the 1st week, and then declined in the 2nd week. There was no significant difference between PNS group and NS group in the 3rd week. The expression of BMP-2 in PNS group was higher than that in NS group (P<0.05) in the 1st week, and reached the peak in the 2 nd week. The expression of BMP-2 was continued to decline in the 3 rd and 4th week, and there was no significant difference between PNS group and NS group. Conclusion: Panaxnot-oginseng saponins can increase the number of osteoclasts in periodontal tissues and increase the expression of BMP-2 in periodontal tissues,which suggests that PNS has a positive effect on the alveolar bone remodeling during teeth movement.

Key words: Panaxnotoginseng saponins, Bone morphogenetic protein-2, Movement of teeth, Osteoclasts