口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (4): 448-451.DOI: 10.13701/j.cnki.kqyxyj.2018.04.025

• 其他研究 • 上一篇    下一篇

雌激素受体对去势大鼠牙周膜干细胞成骨分化能力的影响

沈兰花, 张瑞, 孟玲娜*   

  1. 黑龙江省医院南岗院区口腔科 黑龙江 哈尔滨 150001
  • 收稿日期:2017-11-04 出版日期:2018-04-28 发布日期:2018-04-25
  • 通讯作者: 孟玲娜,电话:0451-88025094
  • 作者简介:沈兰花(1966~ ),女,黑龙江人,博士,主要从事正畸牙对雌激素的影响研究。
  • 基金资助:
    黑龙江省自然科学基金(编号:H2016044)

Effects of Estrogen Receptor on Osteogenic Differentiation of Periodontal Ligament Stem Cells in Ovariectomized Rats.

SHEN Lan-hua, ZHANG Rui, MENG Ling-na*   

  1. Department of Stomatology, Nangang Branch of Heilongjiang Provincial Hospital, Harbin 150001, China.
  • Received:2017-11-04 Online:2018-04-28 Published:2018-04-25

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摘要： 目的:营造低雌激素微环境,通过牙周膜干细胞(PDLSCs)提取,来探讨雌激素受体对PDLSCs成骨分化能力的影响。方法:购入SD雌性大鼠16只,将其随机分为去势组与假手术组,分别给予相应的外科手术处理,完成大鼠模型建立;运用实时定量PCR法考察大鼠PDLSCs提取过程中雌激素受体以及成骨指标的变化。结果:去势组大鼠体重显著高于假手术组,雌二醇、股骨骨密度显著低于假手术组(P<0.05);去势组骨诱导培养第3、5、7、9、11天ALP活性检测结果均高于假手术组(P<0.05),第1、13天无差异;去势组ERα mRNA第5、9、11、13天显著低于假手术组;去势组ERβ mRNA第5、7、11、13天显著低于假手术组;去势组ALP mRNA、BSP mRNA第5、7、9、11、13天均显著低于假手术组(P<0.05)。结论:去势大鼠牙周膜干细胞的成骨能力也会出现相应的减弱,在雌激素介导中,雌激素受体对牙周膜干细胞成骨分化能力发挥了非常重要的调控作用。

关键词: 雌激素受体, 牙周膜干细胞, 成骨分化能力

Abstract: Objective: To investigate the effect of estrogen receptor on osteogenic differentiation of periodontal ligament stem cells (PDLSCs) by constructing a low estrogen microenvironment. Methods: Sixteen female SD rats were randomly divided into ovariectomized group and sham operation group, who were given surgical treatment accordingly. Real-time quantitative PCR was used to measure the rat estrogen receptor and osteogenic index. Results: The weight of OVX group was significantly higher than that in the sham operation group, and estradiol and bone density were significantly lower than those of sham operation group (P<0.05). The ALP activities of OVX group after 3, 5, 7, 9, and 11 d were higher than those of sham operation group (P<0.05), however, no difference at 1d and 13 d. The levels of ERα mRNA of OVX group after 5, 9, 11, and 13 d were significantly lower than those of sham operation group, and the levels of ERβ mRNA of OVX group after 5, 7, 11, and 13 d were significantly lower than those in sham operation group. The levels of ALP mRNA, BSP mRNA of OVX group after 5, 7, 9, 11, and 13 d were significantly lower than those in sham operation group (P<0.05). Conclusion: The osteogenic ability of PDLSCs in ovariectomized rat is correspondingly reduced, and the estrogen receptor plays an important role on osteogenic differentiation ability of PDLSCs.

Key words: Estrogen receptor, Periodontal ligament stem cells, Osteogenic differentiation ability