口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (11): 1165-1167.DOI: 10.13701/j.cnki.kqyxyj.2016.11.013

• 临床研究论著 • 上一篇    下一篇

内皮素-1对牙周膜干细胞肿瘤坏死因子表达的影响

梁莉1,余继锋2,周威1,刘洪臣3*   

  1. 1. 解放军309医院口腔科 北京 100091;
    2. 首都医科大学附属北京儿童医院眼科 北京 100000;
    3. 解放军总医院口腔医学研究所 北京 100000
  • 收稿日期:2016-04-21 出版日期:2016-11-25 发布日期:2016-11-25
  • 通讯作者: 刘洪臣,E-mail: liuhongchen301@hotmail.com
  • 作者简介:梁莉(1981~ ),女,河北人,副主任医师,博士,主要从事口腔正畸学的研究工作。
  • 基金资助:
    国家自然科学基金项目(编号:81500821)

Effect of Endothelin-1 on Expression of Tumor Necrosis Factor of Periodontal Ligament Stem Cells

LIANG Li1, YU Ji-feng2, ZHOU Wei1, LIU Hong-chen3*   

  1. 1. Department of Stomatology, the 309th Hospital of Chinese PLA, Beijing 100091, China;
    2. Department of Ophthalmology, Beijing Children's Hospital, Capital Medical University, Beijing 100000, China;
    3. Institute of Stomatological Research, Chinese PLA General Hospital, Beijing 100000, China
  • Received:2016-04-21 Online:2016-11-25 Published:2016-11-25

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摘要： 目的:探讨内皮素-1(endothelin-1,ET-1)对牙周膜干细胞(periodontal ligament stem cells,PDLSCs)肿瘤坏死因子(Tumor Necrosis Factor,TNF-α)表达的影响。方法:体外培养鉴定牙周膜干细胞,加入不同浓度(1,10,100 nmol/L)的ET-1培养12、24、72 h,以未作任何处理的牙周膜干细胞为对照,采用ELISA及Western blot检测ET-1作用下牙周膜干细胞TNF-α分泌及蛋白表达的改变。结果:与对照组相比,ET-1对牙周膜干细胞TNF-α分泌及蛋白表达具有显著促进作用,且促进作用具有剂量依赖性及时间依赖性。结论:内皮素-1可以诱导牙周膜干细胞分泌TNF-α,而且这种促进作用呈浓度依赖性及时间依赖性。

关键词: 牙周炎, 牙周膜干细胞, 内皮素-1, 肿瘤坏死因子

Abstract: Objective: To investigate how endothelin-1 affects the expression of tumor necrosis factor (TNF-α) in periodontal ligament stem cells (PDLSCs). Methods: The PDLSCs were stimulated with endothelin-1 (1, 10, 100nmol/L) for 12, 24, and 72 hours. The untreated PDLSCs served as normal control. The effect of ET-1 stimulation on TNF-α protein levels was evaluated by ELISA and Western blot. Results: The TNF-α protein levels were enhanced by ET-1 stimulation in a dose-dependent and time-course manner. Conclusion: These results indicate that ET-1 is involved in the regulation of TNF-α expression in PDLSCs and suggest that ET-1 signaling may be a therapeutic target for treating TNF-α-dependent inflammatory responses.

Key words: Periodontitis, Periodontal ligament stem cells, Endothelin-1, Tumor necrosis factor

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