口腔医学研究

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (1): 42-45.DOI: 10.13701/j.cnki.kqyxyj.2019.01.010

• 口腔颌面外科学研究 • 上一篇    下一篇

β-谷甾醇诱导口腔鳞状细胞癌SCC9细胞凋亡及机制研究

裴浩1, 李海朋1, 黄莹莹2, 马钊2*   

  1. 1. 南阳医学高等专科学校附属医院口腔科 河南 南阳 473058;
    2. 郑州大学基础医学院解剖教研室 河南 郑州 450001
  • 收稿日期:2018-07-19 出版日期:2019-01-18 发布日期:2019-01-28
  • 通讯作者: 马钊,E-mail:zhaomazzdxbmc@163.com
  • 作者简介:裴浩(1977~ ),男,河南南阳人,硕士,副主任医师,主要从事口腔科临床工作。
  • 基金资助:
    河南省高校科技创新人才支持计划(编号:16IRTSTHN025)

Molecular Mechanisms of β-sitosterol on Oral Squamous Cell Carcinoma Cells SCC9 Apoptosis

PEI Hao1, LI Hai-peng1, HUANG Ying-ying2, MA Zhao2*   

  1. 1. Department of Stomatology, Affiliated Hospital of Nanyang Medical College, Nanyang 473058, China;
    2. Department of Anatomy, Basic Medical School of Zhengzhou University, Zhengzhou 450001, China
  • Received:2018-07-19 Online:2019-01-18 Published:2019-01-28

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摘要： 目的:研究β-谷甾醇对口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)细胞SCC9的作用及分子机制,以期为β-谷甾醇应用于临床治疗OSCC等肿瘤提供理论支持。方法:采用不同浓度的β-谷甾醇体外作用于培养中的SCC9细胞,观察SCC9的凋亡发生情况,同时检测SCC9胞浆内活性氧(reactive oxygen species,ROS)水平改变及烟酰胺腺嘌呤二核苷酸磷酸(nicotinamide adenine dinucleotide phosphate,NADPH)氧化酶gp91phox基因及蛋白表达水平;通过小干扰RNA(small interference RNA,siRNA)技术抑制SCC9细胞中gp91phox的表达对其功能进行确认。结果: 结果显示,相较于对照组细胞,β-谷甾醇显著诱导其凋亡,且呈浓度依赖性(P<0.01);β-谷甾醇显著提高SCC9胞浆内ROS水平,促进gp91phox的表达(P<0.01);抑制gp91phox表达可阻断β-谷甾醇对SCC9的促凋亡作用(P<0.01)。结论:β-谷甾醇可有效诱导SCC9细胞凋亡,且NADPH氧化酶gp91phox在其中起关键调控作用,可作为临床治疗的潜在靶点之一。

关键词: 口腔鳞状细胞癌, β-谷甾醇, 凋亡, 活性氧, 氧化酶gp91phox

Abstract: Objective: To investigate the effects and related mechanism of β-sitosterol on oral squamous cell carcinoma cells SSC9. Methods: SCC9 cells were treated by various dose of β-sitosterol, and the cell apoptosis was monitored. The intracellular ROS were analyzed and the mRNA and protein level of NADPH oxidase gp91phox were detected. Knock down of gp91phox in SCC9 was performed to confirm the effects of β-sitosterol on SCC9 cells. Results: Compared with the control group, β-sitosterol induced cell apoptosis in a dose dependent manner (P<0.01). β-sitosterol elevated the intracellular ROS level and mRNA and protein expression of gp91phox in SCC9 cells. Inhibition of gp91phox blocked the effect of β-sitosterol on SCC9 cells. Conclusion: NADPH oxidase gp91phox had an important role in mediating the β-sitosterol-induced apoptotic process in SCC9 cells, which might be a target of OSCC treatment.

Key words: Oral squamous cell carcinoma, β-sitosterol, Apoptosis, ROS, Gp91phox