透明质酸对颞下颌关节滑膜细胞氧化应激的保护作用

doi:10.13701/j.cnki.kqyxyj.2019.12.010

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (12): 1141-1144.DOI: 10.13701/j.cnki.kqyxyj.2019.12.010

透明质酸对颞下颌关节滑膜细胞氧化应激的保护作用

蔡恒星^*, 李颖杰, 龙星

武汉大学口腔医学院湖北武汉 430079

收稿日期:2019-01-18 出版日期:2019-12-28 发布日期:2019-12-23
通讯作者: 蔡恒星,电话:027-87686216
作者简介:蔡恒星(1974~ ),男,河南周口人,副教授,副主任医师,主要从事口腔颌面外科临床及基础的研究工作。
基金资助:
国家自然科学青年基金(编号:30901683)教育部博士点新教师基金(编号:20090141120043)

Protective Effects of Hyaluronic Acid on Synovial Fibroblasts from Temporomandibular Joint Against Oxidative Stress Induced by Hydrogen Peroxide

CAI Hengxing^*, LI Yingjie, LONG Xing

School of Stomatology, Wuhan University, Wuhan 430079, China.

Received:2019-01-18 Online:2019-12-28 Published:2019-12-23

摘要/Abstract

摘要： 目的:采用过氧化氢(H₂O₂)模拟颞下颌关节腔内的氧化应激状态,并利用系列浓度的高分子量透明质酸(hyaluronic acid,HA)进行干预,探讨氧化应激对滑膜细胞的影响及HA的干预作用。方法:实验动物为日本大耳白兔,通过外科手术获取颞下颌关节滑膜组织并进行细胞培养。采用系列浓度的H₂O₂进行氧化应激实验,探索合适的H₂O₂实验浓度,将H₂O₂与不同浓度的HA共同作用于培养细胞,观察细胞粘附情况, 并采用噻唑蓝(methyl thiazol tetrazolium,MTT)法观察H₂O₂和不同浓度HA对滑膜细胞增殖的影响。结果:低浓度的H₂O₂(5、10 μmol/L)对细胞活力没有抑制作用(P＞0.05),而100 μmol/L H₂O₂对细胞活力的抑制作用过强(P＜0.05)。50 μmol/L H₂O₂对细胞活力的抑制作用约为对照组的60%,因此选择此浓度为本实验的刺激浓度。中等浓度的HA(0.01%和0.05%)对滑膜细胞氧化应激的保护作用最强,与其他实验组比较差异有统计学意义(P＜0.05);低浓度的HA效果不明显,与单纯H₂O₂组比较差异无统计学意义(P＞0.05);而高浓度的HA抑制滑膜细胞增殖。结论:利用50 μmol/L的H₂O₂可以模拟兔颞下颌关节滑膜细胞氧化应激状态,中等浓度的HA可以对滑膜细胞起到保护作用。

关键词: 颞下颌关节, 滑膜细胞, 氧化应激, 透明质酸, 细胞培养

Abstract: Objective: To observe the effects of oxygen free radicals on the synovial fibroblasts from the temporomandibular joint and the protective effects of hyaluronic acid against the oxidative stress. Methods: Synovial tissues were harvested from the rabbit temporomandibular joint and cultured en bloc. The synovial fibroblasts gained at 3-8 passages were used for further study. Hydrogen peroxide was introduced to mimic the effect of oxygen free radicals within the temporomandibular joint, and the hyaluronic acid was added to counteract the effects of reactive oxygen species. Results: There was no difference in the vitality of synovial fibroblasts between H₂O₂ groups (5 μmol/L and 10 μmol/L) and control group (P＞0.05),while the 100 μmol/L H₂O₂ overinhibited the viability of the synovial fibroblasts. 50μmol/L H₂O₂ appropriately inhibited the activity of synovial fibroblasts (60%) and was selected as the ideal stimulus. In the serial concentrations of hyaluronic acid, the 0.01% and 0.05% groups significantly recovered the activity of synovial fibroblasts in contrast to the low or high concentrations of HA (P＜0.05). Conclusion: Proper oxidative stress to the synovial fibroblasts can be induced by 50 μmol/L H₂O₂, while the 0.01% and 0.05% HA can protect the synovial fibroblasts from the damage of oxygen free radicals.

Key words: temporomandibular joint, synovial fibroblast, oxidative stress, hyaluronic acid, cell culture

蔡恒星, 李颖杰, 龙星. 透明质酸对颞下颌关节滑膜细胞氧化应激的保护作用[J]. 口腔医学研究, 2019, 35(12): 1141-1144.

CAI Hengxing, LI Yingjie, LONG Xing. Protective Effects of Hyaluronic Acid on Synovial Fibroblasts from Temporomandibular Joint Against Oxidative Stress Induced by Hydrogen Peroxide[J]. Journal of Oral Science Research, 2019, 35(12): 1141-1144.

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透明质酸对颞下颌关节滑膜细胞氧化应激的保护作用

Protective Effects of Hyaluronic Acid on Synovial Fibroblasts from Temporomandibular Joint Against Oxidative Stress Induced by Hydrogen Peroxide

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