转录因子XBP1在口腔鳞状细胞癌中的表达

doi:10.13701/j.cnki.kqyxyj.2016.02.008

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (2): 139-142.DOI: 10.13701/j.cnki.kqyxyj.2016.02.008

转录因子XBP1在口腔鳞状细胞癌中的表达

蒋芳艳¹ 王瑜¹ 陈吉荣¹ 孙雅楠¹ 陈新明² 张佳莉^1,2*

1. 口腔基础医学省部共建国家重点实验室培育基地和口腔生物医学教育部重点实验室,
武汉大学口腔医学院湖北武汉 430079;2. 武汉大学口腔医院病理科湖北武汉 430079

收稿日期:2015-07-23 出版日期:2016-02-28 发布日期:2016-03-10
通讯作者: 张佳莉,E-mail:jiali_zhang97@163.com
作者简介:蒋芳艳(1986~),女,广西桂林人,硕士在读,主要从事口腔肿瘤的基础研究。
基金资助:
国家自然科学基金资助项目(编号:81172571

Expression of Transcription Factor XBP1 in Oral Squamous Cell Carcinoma.

JIANG Fang-yan¹, WANG Yu¹, CHEN Ji-rong¹, SUN Ya-nan¹, CHEN Xin-ming², ZHANG Jia-li^1,2.

1. The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China;
2. Department of Pathology, Hospital of Stomatology, Wuhan University, Wuhan 430079, China

Received:2015-07-23 Online:2016-02-28 Published:2016-03-10

摘要/Abstract

摘要： 目的:检测口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)组织中X盒结合蛋白1 (X box binding protein 1,XBP1)的表达,并探讨其意义。方法:应用qRT-PCR法检测50例OSCC肿瘤和癌旁正常黏膜上皮中剪切型XBP1(XBP1 splicing, XBP1-s)的表达;应用组织芯片技术和免疫组化染色相结合的方法检测100例OSCC癌旁上皮、肿瘤中心、侵袭前沿及淋巴结转移灶中XBP1蛋白的表达。结果:qRT-PCR显示:XBP1-s mRNA表达显著上升(log2(倍数)≥1)的病例与颈淋巴结转移相关(P＜0.05)。免疫组化结果显示:XBP1蛋白在OSCC侵袭前沿和淋巴结转移灶中的表达均高于癌旁上皮和肿瘤中心(P＜0.05);且在淋巴结转移灶中的表达明显高于侵袭前沿(P＜0.05)。此外,XBP1蛋白表达还与OSCC组织病理学分级相关(P＜0.05)。结论:XBP1可能是促进口腔鳞状细胞癌侵袭和转移的一个重要因子。

关键词: 口腔鳞状细胞癌, XBP1, 侵袭前沿, 转移

Abstract: Objective: To detect the expression and explore the role of X box binding protein 1 (XBP1) in oral squamous cell carcinoma (OSCC). Methods: Real-time PCR was performed to detect the expression of X box binding protein 1 splicing (XBP1-s) in 50 cases of fresh primary OSCC samples and the corresponding matched epithelial tissues. The tissue microarray technique and immunohistochemistry were used to detect XBP1 protein expression in 100 cases of OSCC samples, and each case included the adjacent normal epithelial, primary tumor center area, invasive front and lymph node metastasis. Results: The relative expression of XBP1-s mRNA with more than 2-fold increase in OSCC tissue was closely related with lymph node metastasis (P＜0.05). Immunohistochemistry staining showed that the expression levels of XBP1 protein in the invasive front and lymph node metastasis were higher compare with that in the epithelial and tumor center (P＜0.05). Additionally, XBP1 protein expression in lymph node metastasis was significantly higher than that in invasive front (P＜0.05). Moreover, XBP1 protein expression was significantly correlated with pathological grade of OSCC tissues (P＜0.05). Conclusion: XBP1 might play an important role in OSCC invasion and promote tumor metastasis.

中图分类号:

R739.8

蒋芳艳,王瑜,陈吉荣,孙雅楠,陈新明,张佳莉. 转录因子XBP1在口腔鳞状细胞癌中的表达[J]. 口腔医学研究, 2016, 32(2): 139-142.

JIANG Fang-yan, WANG Yu, CHEN Ji-rong, SUN Ya-nan, CHEN Xin-ming, ZHANG Jia-li.. Expression of Transcription Factor XBP1 in Oral Squamous Cell Carcinoma.[J]. Journal of Oral Science Research, 2016, 32(2): 139-142.

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转录因子XBP1在口腔鳞状细胞癌中的表达

Expression of Transcription Factor XBP1 in Oral Squamous Cell Carcinoma.

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