纳米羟基磷灰石表面阿伦膦酸钠和I型胶原蛋白复合膜的制备

doi:10.11839/kqyxzh.2016.01.006

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (1): 21-25.DOI: 10.11839/kqyxzh.2016.01.006

纳米羟基磷灰石表面阿伦膦酸钠和I型胶原蛋白复合膜的制备

姜燕,姚江武^*

厦门市口腔医院修复科福建厦门 361003

收稿日期:2015-06-25 出版日期:2016-01-28 发布日期:2016-03-21
通讯作者: 姚江武,E-mail:dentyjw@126.com
作者简介:姜燕(1988~),女,汉族,福建人,住院医师,硕士,主要从事口腔修复学和口腔生物材料学研究。
基金资助:
福建省自然科学基金面上项目(编号:2013D010)

Formation of Alendronate Sodium and Collagen Type I Complex Films on the Nano-hydroxyapatite Surface.

JIANG Yan, YAO Jiang-Wu.

Xiamen Stomatology Hospital. Xiamen 361003, China

Received:2015-06-25 Online:2016-01-28 Published:2016-03-21

摘要/Abstract

摘要： 目的:在纳米羟基磷灰石(nHA)涂层表面制备稳定可重复的阿伦膦酸钠(ALN)和I型胶原蛋白(COL I)复合膜。方法:在纯钛(PT)表面电化学沉积nHA涂层,以3-氨丙基三乙氧基硅烷(APTES)、1-乙基-(3-二甲基氨基丙基)碳二亚胺盐酸盐(EDC)为偶联剂,在涂层表面结合ALN和COL I,制备nHA/ALN/COL I和nHA/COL I/ALN复合膜。通过扫描电子显微镜(SEM),X射线能谱分析仪(EDS)和X射线衍射仪(XRD)表征nHA涂层,通过X射线光电子能谱仪(XPS)和傅里叶红外光谱仪(FTIR)表征复合膜。结果:ALN和COL I可通过偶联剂结合于nHA涂层,改变其结合次序,可分别形成nHA/ALN/COL I和nHA/COL I/ALN复合膜。结论:通过化学结合法形成的nHA/ALN/COL I和nHA/COL I/ALN复合膜具有不同的ALN缓释潜力。

关键词: 纳米羟基磷灰石, 阿伦膦酸钠, I型胶原蛋白, 表面修饰

Abstract: Objective: To form a stable and repeatable alendronate sodium (ALN) and collagen type I (COL I) complex films on nano-hydroxyapatite (nHA) layer. Methods: A nHA layer was coated on the pure titanium (PT) surface by using electrochemical deposition, then ALN and COL I were chemically bonded on the nHA layer to form nHA/ALN/COL I and nHA/COL I/ALN complex films using 3-aminopropyl triethoxysilane (APTES) and 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) as coupling agent. The nHA layer was characterized by scanning electron microscope (SEM), energy dispersive X-ray spectroscopy (EDS) and X-ray diffractometer (XRD), and the complex films were characterized by X-ray photoelectron spectrometer (XPS) and Fourier transform infrared spectroscopy (FTIR). Results: ALN and COL I were bonded on the nHA layer by coupling agent, nHA/ALN/COL I and nHA/COL I/ALN complex films were formed by changing the binding order of ALN and COL I. Conclusion: The nHA/ALN/COL I and nHA/COL I/ALN complex films formed by chemical covalent bonding method have different potentials of ALN sustained-release.

中图分类号:

R783.1

姜燕,姚江武. 纳米羟基磷灰石表面阿伦膦酸钠和I型胶原蛋白复合膜的制备[J]. 口腔医学研究, 2016, 32(1): 21-25.

JIANG Yan, YAO Jiang-Wu.. Formation of Alendronate Sodium and Collagen Type I Complex Films on the Nano-hydroxyapatite Surface.[J]. Journal of Oral Science Research, 2016, 32(1): 21-25.

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纳米羟基磷灰石表面阿伦膦酸钠和I型胶原蛋白复合膜的制备

Formation of Alendronate Sodium and Collagen Type I Complex Films on the Nano-hydroxyapatite Surface.

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