口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (1): 17-20.DOI: 10.11839/kqyxzh.2016.01.005

• 基础研究论著 • 上一篇    下一篇

PMX-53明胶复合纳米微粒制备及抗炎作用的体外研究

李娜1,倪世磊2,侯玉帛1,刘歆婵3,唐秋玲1,潘佳慧1,李格格1,于维先2*   

  1. 1. 吉林大学口腔医学院牙周病科 吉林 长春 130021;
    2. 吉林省牙发育及颌骨重塑与再生重点实验室 吉林 长春 130021;
    3. 吉林大学口腔医学院种植科 吉林 长春 130021
  • 收稿日期:2015-08-10 出版日期:2016-01-28 发布日期:2016-03-21
  • 通讯作者: 于维先,电话:0431-88796000
  • 作者简介:李娜(1990~),女,吉林人,医师,硕士,主要从事口腔牙周病学临床和基础研究工作。
  • 基金资助:
    基金项目吉林大学研究生创新基金资助项目(编号:2015090)
    吉林省科技厅自然科学基金项目(编号:20150101076JC)

Preparation and Anti-inflammatory Effects of Nanoparticles Loaded with PMX-53.

LI Na1, NI Shi-lei2, HOU Yu-bo1, LIU Xin-chan3, TANG Qiu-ling1, Pan Jia-hui1, LI Ge-ge1, YU Wei-xian2.   

  1. 1. Department of Periodontics, School of Stomatology, Jilin University, Changchun 130021, China;
    2. Key Lab for Tooth Development and Regenerative of Jaw of Jilin Province, Changchun 130021, China; 3. Department of Implantology, School of Stomatology, Jilin University, Changchun 130021, China
  • Received:2015-08-10 Online:2016-01-28 Published:2016-03-21

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摘要： 目的:探讨PMX-53明胶复合纳米微粒的制备工艺及其抗炎作用。方法:采用两步去溶剂法制备PMX-53明胶复合纳米微粒,SEM观察载药微粒的形态、粒径分布、分散度;高效液相色谱法检测载药微粒包封率、载药量及缓释规律。体外将小鼠巨噬细胞(RAW264.7)与牙龈素共培养模拟炎症环境,观察载药微粒抗炎效果,实验分阴性对照组、牙龈素组、牙龈素+PMX-53(1 mg/L)组及牙龈素+载药微粒(4.17 g/L)组,培养48 h,通过酶联免疫吸附法检测炎症介质IL-1β和IL-6水平。结果:SEM显示PMX-53明胶复合纳米微粒形态圆整,分散度好,直径40～60 nm载药微粒占51.24%,载药量达0.24 mg/g,包封率46.2%,且PMX-53明胶复合纳米微粒药物释放时间达7 d。ELISA检测结果显示,体外细胞培养48 h后,牙龈素组的IL-1β和IL-6分泌量较阴性对照组增加,而牙龈素+PMX-53组和牙龈素+载药微粒组的IL-1β和IL-6分泌量均较牙龈素组减少,其中牙龈素+载药微粒组的IL-6分泌量明显减少。结论:制备的PMX-53明胶复合纳米微粒体外抗炎作用好。

关键词: 牙周炎, PMX-53, 明胶, 纳米微粒

Abstract: Objective: To prepare a sustained-release nanoparticles loaded with PMX-53. Methods: Nanoparticles loaded with PMX-53 were prepared by a two-step desolvation process. The morphology of the nanoparticles was observed by SEM. The encapsulation rate, drug content and the drug release were tested by HPLC. RAW264.7 cells were cocultured with gingipains to simulate inflammation environment and study the anti-inflammatory effects of drug-loaded microparticles in vitro. The experiment was divided into 4 groups: control group, gingipains group, gingipains+PMX-53 (1mg/L) group, and gingipains+nanoparticles loaded with PMX-53 (4.17g/L) group. The levels of IL-1β and IL-6 were detected after 48 hours by enzyme linked immunosorbent assay (ELISA). Results: SEM revealed that nanoparticles loaded with PMX-53 were spherical and 51.24% of them were 40~60nm in diameter. Drug content was 0.24mg/g. The encapsulation rate was 46.2%. The release cycle was up to seven days. The levels of IL-1β and IL-6 of gingipains group were higher than those of control group after 48 hours by ELISA. The levels of IL-1β and IL-6 of gingipains+nanoparticles loaded with PMX-53 group and gingipains+PMX-53 group were lower than gingipains group. Furthermore, there was a significant reduction in the secretion of IL-6 of gingipains+nanoparticles loaded with PMX-53 group. Conclusion: Nanoparticles loaded with PMX-53 showed good anti-inflammatory effects.

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