纳米羟基磷灰石表面阿伦膦酸钠和I型胶原蛋白复合膜对成骨细胞形态和增殖的影响

doi:10.13701/j.cnki.kqyxyj.2016.03.010

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (3): 253-256.DOI: 10.13701/j.cnki.kqyxyj.2016.03.010

纳米羟基磷灰石表面阿伦膦酸钠和I型胶原蛋白复合膜对成骨细胞形态和增殖的影响

姜燕, 姚江武

厦门市口腔医院修复科福建厦门 361003

收稿日期:2015-06-25 出版日期:2016-03-28 发布日期:2016-03-29
通讯作者: 姚江武,E-mail: dentyjw@126.com
作者简介:姜燕(1988~ ),女,汉族,福建人,住院医师,硕士,主要从事口腔修复学和口腔生物材料学研究。
基金资助:
福建省自然科学基金面上项目(编号:2013D010)

Effect of Alendronate sAium and Collagen Type I Complex Films of Nano-hydroxyapatite Surface on Osteoblasts Morphology and Proliferation.

JIANG Yan, YAO Jiang-Wu^*

Xiamen Dental Hospital, Xiamen 361003, China

Received:2015-06-25 Online:2016-03-28 Published:2016-03-29

摘要/Abstract

摘要： 目的: 探讨经化学结合阿伦膦酸钠(alendronate sAium,ALN)和I型胶原蛋白(collagen type I,COL I)修饰的纳米羟基磷灰石(nano-hydroxyapatite,nHA)涂层对成骨细胞在其表面黏附、生长和增殖的影响。方法: 建立nHA涂层、nHA/ALN、nHA/COL I、nHA/ALN/COL I和nHA/COL I/ALN复合膜,将成骨细胞接种于复合膜表面,分别培养7 d和14 d,扫描电镜观察成骨细胞在不同膜层表面的黏附形态的变化,WST-1法对各膜层表面细胞数量进行定量检测。结果: 成骨细胞在nHA/ALN/COL I和nHA/COL I/ALN复合膜表面的增殖活性最高(P<0.05),扫描电镜观察成骨细胞在nHA/ALN/COL I和nHA/COL I/ALN复合膜表面生长状态优于其他膜层,在COL I膜直接作用下,成骨细胞伸展更为完全,并有较多伪足突起和微绒毛结构呈分化表型。结论: 将ALN和COL I 修饰于nHA表面能够协同促进成骨细胞的黏附和增殖。nHA/ALN/COL I复合膜促进成骨细胞的黏附与分化。

关键词: 纳米羟基磷灰石, 阿伦膦酸钠, I型胶原蛋白, 成骨细胞

Abstract: Objective: To investigate osteoblast adhesion, growth and proliferation on the alendronate sAium (ALN) and collagen type I (COL I) coated nano-hydroxyapatite (nHA) surface. Methods: nHA layer, nHA/ALN complex film, nHA/COL I complex film, nHA/ALN/COL I complex film, and nHA/COL I/ALN complex film were established. Osteoblasts were cultured on the films for 7 days and 14 days. The morphology of osteoblast was observed by SEM and the cell proliferation was determined by WST-1 assay, respectively. Results: The results revealed that nHA/ALN/COL I and nHA/COL I/ALN complex films accelerated osteoblasts growth and proliferation rate (P<0.05). The osteoblasts showed fully extended morphology combined with numerous pseudopodia and microvilli structure by directly contact to the collagen layer. Conclusion: ALN and COL I exhibited synergistic effect in accelerating adhesion and proliferation of osteoblasts in vitro. The nHA/ALN/COL I complex film benefited osteoblast adhesion and differentiation.

Key words: Nano-hydroxyapatite, Alendronate sAium , Collagen type I , Osteoblast

中图分类号:

R783.1

姜燕, 姚江武. 纳米羟基磷灰石表面阿伦膦酸钠和I型胶原蛋白复合膜对成骨细胞形态和增殖的影响[J]. 口腔医学研究, 2016, 32(3): 253-256.

JIANG Yan, YAO Jiang-Wu. Effect of Alendronate sAium and Collagen Type I Complex Films of Nano-hydroxyapatite Surface on Osteoblasts Morphology and Proliferation.[J]. Journal of Oral Science Research, 2016, 32(3): 253-256.

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纳米羟基磷灰石表面阿伦膦酸钠和I型胶原蛋白复合膜对成骨细胞形态和增殖的影响

Effect of Alendronate sAium and Collagen Type I Complex Films of Nano-hydroxyapatite Surface on Osteoblasts Morphology and Proliferation.

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