正畸力作用下压力侧Wnt10b、RANKL在牙周组织中的表达

doi:10.13701/j.cnki.kqyxyj.2016.03.011

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (3): 257-260.DOI: 10.13701/j.cnki.kqyxyj.2016.03.011

正畸力作用下压力侧Wnt10b、RANKL在牙周组织中的表达

王莹¹, 王兰珠², 韩博², 吴明明², 张苗苗¹

1.哈尔滨医科大学附属口腔医院正畸科黑龙江哈尔滨 150001;
2. 哈尔滨医科大学附属第一医院黑龙江哈尔滨 150000

收稿日期:2015-10-19 出版日期:2016-03-28 发布日期:2016-03-29
通讯作者: 张苗苗,E-mail:zhangmiaohlj@163.com
作者简介:王莹(1989~ ),女,牡丹江人,硕士,医师,主要从事口腔正畸临床工作。
基金资助:
黑龙江省博士后启动基金(项目编号:LBH-Q11033)
哈尔滨医科大学附属第一医院博士基金(项目编号:2009B29)

Expression of Wnt10b and RANKL in the Pressure Site of Periodontal Tissue under Orthodontic Force.

WANG Ying¹, WANG Lan-zhu², HAN Bo², WU Ming-ming², ZHANG Miao-miao^1*

Department of Orthodontics, the Affiliated Stomatology Hospital of Harbin Medical University, Harbin 150001, China

Received:2015-10-19 Online:2016-03-28 Published:2016-03-29

摘要/Abstract

摘要： 目的: 研究大鼠在正畸力作用下压力侧wnt10b、RANKL 在牙周组织中的表达变化。方法: 将42只健康雄性SD大鼠随机分为7组,即0 h、6 h、12 h、24 h、5 d、7 d、14 d组,使用镍钛拉簧对第一磨牙向近中施加50 g正畸力,以0 h为对照组,每组处死6只。应用实时定量PCR(RT-PCR)对wnt10b、RANKL的mRNA表达进行检测。结果: Real-time PCR结果显示,与对照组相比,实验组压力侧牙周组织中Wnt10b、RANKL mRNA均有表达,其中wnt10b于加力初期表达受抑制,随后升高,于5 d达高峰随后下降,14 d恢复至正常水平。RANKL表达随加力时间延长而逐渐升高,7 d达到高峰,随后下降。结论: Wnt10b、RANKL参与正畸加力作用下的牙周组织改建。

关键词: 正畸牙移动, 破骨细胞分化, 牙周组织改建, Wnt10b, RANKL

Abstract: Objective: To investigate the changes of RANKL and wnt10b in the periodontal tissue during orthodontic tooth movement. Methods: 〖WT5”BZ〗Forty two healthy male SD rats were randomly divided into 7 groups according to the application loaded time points of 0h, 6h, 12h, 24h, 5d, 7d, and 14d. The group of 0h served as control and the Nickel-titanium closed-coils springs were used to produce 50g power to drag the first molars mesially. Real-time PCR (RT-PCR) technology was used to detect the expression of Wnt10b and RANKL. Results: 〖WT5”BZ〗RANKL and Wnt10b were both expressed in the pressure site in comparison with the control group according to the results of the RT-PCR. The expression of wnt10b mRNA was suppressed at the early stage, then increased, and reached the peak after 5-7 days. It returned to the normal level after 14 days. The expression of RANKL elevated at first, then peaked after 5-7 days, and decreased. Conclusion: 〖WT5”BZ〗Wnt10b and RANKL participated in periodontal tissue remodeling under the action of the orthodontic force.

Key words: Orthodontic tooth movement , Osteoclast differentiation , Periodontal tissue remodeling , Wnt10b , RANKL

中图分类号:

R783.5

王莹, 王兰珠, 韩博, 吴明明, 张苗苗. 正畸力作用下压力侧Wnt10b、RANKL在牙周组织中的表达[J]. 口腔医学研究, 2016, 32(3): 257-260.

WANG Ying, WANG Lan-zhu, HAN Bo, WU Ming-ming, ZHANG Miao-miao. Expression of Wnt10b and RANKL in the Pressure Site of Periodontal Tissue under Orthodontic Force.[J]. Journal of Oral Science Research, 2016, 32(3): 257-260.

参考文献

[1] S.Henneman JW, Von don Hoff and JC. maltha. Mechanobiology of tooth movement [J]. Journal of orthodontics, 2008, 30∶299-306
[2] Lim WH, Liu B, Cheng D, et al. Wnt signaling regulates homeostasis of the periodontal ligament [J]. J Periodontal Res, 2014, 49(6)∶751-759
[3] Kuniaki Ota, Patric Q, Ming Ruan, et al. TGF-βinduces wnt10b in osteoclasts from female mice to enhance coupling to osteoblast [J]. Endocrinology, 2013(10), 154(10)∶3745-3752
[4] kukait T, Bram C.J. van der eerden, prapaporm J, et al. Thrumbin receptor deficiency leads to a high bone mass phenotype by increasing the RANKL/OPG ratio [J]. Bone,2015,27∶14-22
[5] Nakao K, Goto T, Gunjigake KK, Konoo T, Kobayashi S, Yamaguchi K. Intermittent force induces high RANKL expression in human periodontal ligament cells [J]. J Dent Res, 2007, 86∶623-8
[6] 胡江天,李松,沈绍莹. 自制大鼠正畸牙移动模型的建立[J].昆明医学院学报,2009(3)∶25-28
[7] Yasuko M, taru K, Keita M, et al. Histomorphometric study on the effects of age on orthodontic tooth movement and alveolar bone turnover in rats [J]. Eur J Oral Sci,2007,115∶124-130
[8] William J.Boyle, W.ScottS, David L.Lacey. Osteoclast differentiation and activation [J]. Nature,2003,5;15∶423
[9] Patricia J B, Dorrin N, Morris F M , et al.Molecular markers of early tooth movement [J]. Angle Orthod, 2009, 79∶1108-1113
[10] 王威,刘郁,王邦康.大鼠正畸压力侧牙槽骨改建中RANKL和OPG mRNA的表达[J].北京口腔医学,2009,17(2)∶71-75
[11] Rooker SM, Liu B, Helms JA. Role of Wnt signaling in the biology of the periodontium [J]. Dev Dyn, 2010, 239(1)∶140-147
[12] Sheri L. Holmen, Cassandra R. Zylstra, et al. Essential role of β-catenin in postnatal bone acquisition [J]. The journal of biological chemistry,2005,280;22∶21161-21168
[13] Glass D A, Bialek P, Ahn J D, et al. Canonical wnt signaling in differentiated osteoblasts controls osteoclast differentiation [J]. Dev Cell,2005,8∶751-64
[14] Christina N.Bennett, Kenneth A.longo,Wenda S.wright, et al. Regulation of osteoblastogenesis and bone mass by wnt10b [J]. PANS, 2005, 102(9)∶3324-3329
[15] 卢娟,段颖莹,吴明明.大鼠正畸牙移动过程中wnt3a和DKK1的表达[J].口腔医学研究,2015,31(3)∶474-477
[16] William P. Cawthorn, Adam J. Bree, Yao Yao, et al .Wnt6,wnt10a and wnt10b inhibit adipogenesis and stimulate osteoblastogenesis through a β-catenin-dependent mechanism [J]. Bone, 2012, 50(2)∶477-489

正畸力作用下压力侧Wnt10b、RANKL在牙周组织中的表达

Expression of Wnt10b and RANKL in the Pressure Site of Periodontal Tissue under Orthodontic Force.

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	杨安迪, 毛慧敏, 雷浪. 成人双颌前突患者正畸前后上颌切牙区唇腭侧牙槽骨的变化[J]. 口腔医学研究, 2021, 37(1): 48-52.
[2]	刘萍萍, 唐小莹, 袁小平. 基质细胞趋化因子-1对人牙周膜干细胞趋化因子受体——CXC亚家族受体4表达的影响研究[J]. 口腔医学研究, 2020, 36(1): 51-55.
[3]	赵云鹤,李娇,雷浪,李厚轩. 牙槽骨微穿孔术促进大鼠正畸牙移动的机制研究[J]. 口腔医学研究, 2018, 34(9): 960-963.
[4]	唐小莹, 龙杨, 袁小平. Adrb2激动剂、拮抗剂对大鼠牙移动中牙槽骨微结构改建的影响研究[J]. 口腔医学研究, 2018, 34(11): 1217-1222.
[5]	李猷, 张淋坤, 张锡忠, 康永歌, 马遥. 大鼠正畸牙移动中NF-κB通路相关差异基因的GO分析[J]. 口腔医学研究, 2017, 33(8): 832-836.
[6]	林鹏,郭新星,魏竹亮. 局部注射黄芪多糖对大鼠正畸牙保持阶段BMP-2表达的影响[J]. 口腔医学研究, 2017, 33(5): 520-524.
[7]	沈兰花，孟玲娜，张瑞. 正畸牙移动对大鼠血清及局部牙周组织雌激素水平影响的研究[J]. 口腔医学研究, 2017, 33(3): 266-268.
[8]	黄鹂,周泽渊,王欣,宿晨曦,邹淑娟. 组织蛋白酶K抑制剂对正畸牙移动牙周组织改建的影响[J]. 口腔医学研究, 2017, 33(12): 1250-1253.
[9]	韦颂观,陈慧鸿,庞博,谢柳蓉,吴修团,李文良,廖红兵. RNAi技术沉默大鼠骨髓间充质干细胞OPG基因对RANKL/OPG表达变化研究[J]. 口腔医学研究, 2017, 33(12): 1254-1257.
[10]	周骢,伍宝琴,朱中焰,谭红. 正畸移动大鼠牙过程牙周组织中PLAP-1、BMP-2的表达及意义[J]. 口腔医学研究, 2017, 33(1): 33-37.
[11]	邢泉,秦伟,张晓磊,樊明文. IL-21通过Notch信号调控人牙周膜细胞RANKL表达和增殖的研究[J]. 口腔医学研究, 2016, 32(5): 460-463.
[12]	余科, 吴湘楠, 刘文佳, 王航. LPS刺激小鼠骨细胞RANKL/OPG和IL-6表达的实验研究[J]. 口腔医学研究, 2016, 32(3): 220-223.
[13]	许潾于,彭昕欣,雷浪,黄鹏程,张端强. 正畸牙移动过程中高迁移率族蛋白B1的改变[J]. 口腔医学研究, 2015, 31(9): 894-897.
[14]	卢娟, 段颖莹, 吴明明, 王莹, 张苗苗. 大鼠正畸牙齿移动过程中Wnt3a和DKK1的表达[J]. 口腔医学研究, 2015, 31(5): 448-450.
[15]	张江琳,王浩宇,李丹,杨相笛,石磊,罗宁,陈悦. 复方黄芩片对人牙周膜细胞及牙周炎牙槽骨OPG/RANKL表达的影响[J]. 口腔医学研究, 2015, 31(11): 1064-1068.