口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (12): 1254-1257.DOI: 10.13701/j.cnki.kqyxyj.2017.12.004

• 基础研究论著 • 上一篇    下一篇

RNAi技术沉默大鼠骨髓间充质干细胞OPG基因对RANKL/OPG表达变化研究

韦颂观,陈慧鸿,庞博,谢柳蓉,吴修团,李文良,廖红兵*   

  1. 广西医科大学附属口腔医学院口腔修复科 广西 南宁 530021
  • 收稿日期:2017-06-22 出版日期:2017-12-20 发布日期:2018-01-03
  • 通讯作者: 廖红兵,E-mail:dr_liaohb2005@126.com
  • 作者简介:韦颂观(1990~ ),男,广西南宁人,硕士,主要从事口腔修复学及骨组织工程方面的研究。
  • 基金资助:
    国家自然基金资助项目(编号:81560190)；广西自然科学基金(编号:2016GXNSFAA380289)

Effect of RNAi Silencing OPG Gene on RANKL/OPG Expression in Rat Bone Marrow Mesenchymal Stem Cells.

WEI Song-guan, CHEN Hui-hong, PANG Bo, XIE Liu-rong, WU Xiu-tuan, LI Wen-liang, LIAO Hong-bing*   

  1. Department of Prosthodontics, College of Stomatology, Guangxi Medical University, Nanning 530021, China.
  • Received:2017-06-22 Online:2017-12-20 Published:2018-01-03

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被引次数

1

摘要： 目的: 探讨RNAi技术体外沉默大鼠骨髓间充质干细胞(BMSCs)OPG基因提高RANKL/OPG比例的可行性。方法: 设立空载组(空慢病毒载体转染BMSCs)和OPG沉默组(含OPG基因干扰片段的慢病毒载体转染BMSCs),RT-PCR检测OPG及RANKL的mRNA表达变化,Western blot检测OPG及RANKL的蛋白表达变化。结果: 与空载组比较,OPG沉默组OPG的mRNA和蛋白表达显著降低,RANKL的mRNA表达显著升高,差异均有统计学意义(P<0.05);两组RANKL的蛋白表达差异无统计学意义(P>0.05);OPG沉默组RANKL/OPG mRNA和蛋白表达比例均显著升高,差异均有统计学意义(P<0.05)。结论: 以慢病毒为载体,通过RNAi技术体外沉默大鼠骨髓间质干细胞OPG基因提高RANKL/OPG比例的方法是可行的。

关键词: 骨髓间充质干细胞, OPG, RNAi, RANKL, RANKL/OPG

Abstract: Objective: To explore the feasibility of increasing the RANKL/OPG ratio by using the RNAi technique to silence rat OPG gene of bone marrow mesenchymal stem cells (BMSCs) in vitro. Methods: BMSCs were set up in the empty vector group and OPG silencing group. RT-PCR was used to detect the mRNA expression of OPG and RANKL. Western blot was used to detect the protein expression of OPG and RANKL. Results: The expressions of OPG mRNA and protein in OPG silencing group were significantly inhibited and the expression of RANKL mRNA was significantly increased (P<0.05) compared with the empty vector group. There was no significant difference in the expressions of RANKL protein between two groups (P>0.05). The expressions of RANKL/OPG mRNA and protein in OPG silencing group were significantly increased. The difference was statistically significant (P<0.05). Conclusion: It is feasible to increase the RANKL/OPG ratio by using the lentivirus as vector and silencing OPG gene of rat BMSCs by RNAi technique in vitro.

Key words: Bone mesenchymal stem cells , OPG , RNAi, RANKL , RANKL/OPG

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