IL-21通过Notch信号调控人牙周膜细胞RANKL表达和增殖的研究

doi:10.13701/j.cnki.kqyxyj.2016.05.009

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (5): 460-463.DOI: 10.13701/j.cnki.kqyxyj.2016.05.009

IL-21通过Notch信号调控人牙周膜细胞RANKL表达和增殖的研究

邢泉¹ ,秦伟^1# ,张晓磊¹ ,樊明文^2*

1. 中山大学光华口腔医学院附属口腔医院广东省口腔医学重点实验室广东广州 510055;
2. 武汉大学口腔医学院牙体牙髓科,湖北省口腔基础医学重点实验室-省部共建国家重点实验室培育基地口腔生物医学教育部重点实验室湖北武汉 430079

收稿日期:2016-04-05 出版日期:2016-05-26 发布日期:2016-05-26
通讯作者: 樊明文,电话:027-87647443
作者简介:邢泉(1978~ ),男,贵州人,博士,主治医师,主要从事牙体牙髓病学的研究。秦伟(1976~ ),男,安徽人,博士,副主任医师,主要从事牙体牙髓病学的研究。
基金资助:
国家自然科学基金项目(编号:81300874)
广东省医学科研基金项目(编号:B2013152,A2014255)

IL-21 Regulates RANKL Expression and Proliferation Activity in Human Periodontal Ligament Cells via Notch Signal Pathway

XING Quan¹, QIN Wei^1#, ZHANG Xiao-lei¹, FAN Ming-wen^2*.

1. Guangdong Key Laboratory of Stomatology, Guanghua School and Hospital of Stomatology, Sun Yat sen University, Guangzhou 510055, China;
2. Department of Endodontics, School &Hospital of Stomatology, Wuhan University , Hubei-MOST KLOS & KLOBME, School&Hospital of Stomatology, Wuhan University, Wuhan 430079, China

Received:2016-04-05 Online:2016-05-26 Published:2016-05-26

摘要/Abstract

摘要： 目的:观察IL-21对人牙周膜成纤维细胞RANKL/OPG的表达以及增殖活性的影响,以及Notch信号通路在其中的作用,探讨IL-21在根尖周炎骨破坏中的作用机制。方法:使用不同浓度(0、10、50、100 μg/L)的IL-21作用于人牙周膜成纤维细胞,并选择最佳刺激浓度IL-21(50 μg/L)处理人牙周膜成纤维细胞,观察不同的时间点(0、12、24 h),通过实时定量PCR和ELISA的方法检测细胞中RANKL/OPG的表达水平。同时使用实时定量PCR和Western-blot检测IL-21对人牙周膜成纤维细胞Notch1表达水平的影响。运用Notch信号的阻断剂GSI(5 μmol/L)预处理人牙周膜成纤维细胞后,再检测IL-21对人牙周膜成纤维细胞RANKL/OPG的表达水平的影响。CCK8法测定各个实验组细胞增殖活性。结果:IL-21可显著提高人牙周膜成纤维细胞RANKL的表达水平,对OPG的表达无显著影响。IL-21显著抑制人牙周膜成纤维细胞的增殖活性。结论:IL-21通过Notch信号通路上调人牙周膜成纤维细胞的RANKL表达,抑制其增殖活性。

关键词: IL-21, Notch, 人牙周膜成纤维细胞, RANKL, 增殖

Abstract: Objective: To explore the effect of interleukin-21 (IL-21) via Notch signal pathway on the expression of RANKL/OPG and proliferation in hPDLCs and to investigate the possible role of IL-21 in bone resorption of apical periodontitis. Methods: hPDLCs were treated with IL-21 in different concentrations (0, 10, 25, 50 μg/L). Then the optimal concentration (50μg/L) was used in the time-dependent experiment. The expressions of RANKL/OPG were detected by real-time PCR and ELISA. The expressions of RANKL/OPG were detected by real-time PCR and ELISA. Real-time PCR and western-blot were conducted to detect the expression of Notch1 in hPDLCs treated by IL-21. After pretreatment with GSI (5μmol/L) to inhibit Notch1 signaling pathway, the expressions of RANKL/OPG of hPDLCs treated with IL-21 (50μg/L) were investigated. CCK8 was carried out to detect the proliferation activity in the different groups of hPDLCs. Results: The expressions of RANKL were significantly up-regulated by IL-21. IL-21 decreased the cell proliferation activity in hPDLCs. Conclusion: IL-21 up-regulated the expression of RANKL and reduced proliferation activity in hPDLCs via Notch signal pathway.

中图分类号:

R780.1

邢泉,秦伟,张晓磊,樊明文. IL-21通过Notch信号调控人牙周膜细胞RANKL表达和增殖的研究[J]. 口腔医学研究, 2016, 32(5): 460-463.

XING Quan, QIN Wei, ZHANG Xiao-lei, FAN Ming-wen.. IL-21 Regulates RANKL Expression and Proliferation Activity in Human Periodontal Ligament Cells via Notch Signal Pathway[J]. Journal of Oral Science Research, 2016, 32(5): 460-463.

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IL-21通过Notch信号调控人牙周膜细胞RANKL表达和增殖的研究

IL-21 Regulates RANKL Expression and Proliferation Activity in Human Periodontal Ligament Cells via Notch Signal Pathway

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