口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (5): 491-495.DOI: 10.13701/j.cnki.kqyxyj.2017.05.007

• 基础研究论著 • 上一篇    下一篇

银杏叶提取物对成骨细胞分化的影响及相关机制研究

张晓晓1,2,李婧1,王俊妹1,李彤1,杨沫扬3,吴哲1,2,3*   

  1. (1. 吉林大学口腔医学院修复科 吉林 长春 130021;
    2. 吉林省牙发育及颌骨重塑与再生重点实验室 吉林 长春 130021;
    3. 广州医科大学口腔医学院修复科 广东 广州 510140)
  • 收稿日期:2016-12-15 出版日期:2017-05-20 发布日期:2017-05-26
  • 通讯作者: 吴哲,电话:020-61350505
  • 作者简介:张晓晓(1990~ ),女,山西人,硕士,主要从事口腔修复学及骨组织工程方面的研究。

Effects and Likely Molecular Mechanism of Ginkgo Biloba Extract on Osteogenic Differentiation of Osteoblasts.

ZHANG Xiao-xiao1,2, LI Jing1, WANG Jun-mei1, LI Tong1, YANG Mo-yang3, WU Zhe1,2,3*.   

  1. 1. Department of Prosthodontics, School and Hospital of Stomatology, Jilin University, Changchun 130021, China; 2. Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun 130021, China; 3. Department of Prosthodontics, Stomatology School of Guangzhou Medical University, Guangzhou 510140, China.
  • Received:2016-12-15 Online:2017-05-20 Published:2017-05-26

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被引次数

1

摘要： 目的:探讨银杏叶提取物(ginkgo biloba extract,GBE)对成骨细胞分化的影响及其可能的相关分子机制。方法:将不同浓度的GBE作用于MG63,通过茜素红染色及碱性磷酸酶(ALP)活性检测判定其对细胞成骨分化能力的影响。RT-PCR检测Runx2(runt-related transcription factor 2)、一型胶原蛋白(COL1A1)、骨钙素(Osteocalcin,OC) 及细胞周期蛋白D1(cyclinD1)的表达,Western Blot检测细胞核蛋白β-catenin及cyclinD1的表达。结果:不同浓度的GBE能提高ALP活性及钙结节数量,在浓度为75 mg/L时最为显著;此外,GBE能显著提高Runx2、COL1A1、OC、cyclinD1的mRNA表达及cyclinD1的蛋白表达,同时促进β-catenin蛋白的核内转移;而上述活性均能被DKK1所抑制。结论:银杏叶提取物对成骨细胞的分化具有积极意义,其机制可能与经典Wnt通路的激活有关。

关键词: 银杏叶提取物, 成骨分化, 经典Wnt信号通路, 成骨细胞

Abstract: Objective: To investigate the effect and possible mechanisms of GBE(ginkgo biloba extract)on the differentiation of osteoblast. Methods: Different concentrations of GBE were applied to the MG63. The osteogenic differentiation capacity was evaluated by Alizarin red and alkaline phosphatase (ALP) activity. RT-PCR was taken to test the expression of COL1A1 (type I collagen), OC (osteocalcin), Runx2, and cyclinD1. Western blot was adopted to inspect the protein level of cyclinD1 and subcellular localization of β-catenin. Results: Incubation of MG63 cell in GBE-supplemented culture medium significantly increased ALP activity, the mRNA expression levels of COL1A1, OC, Runx2, and cyclinD1 as well as the cyclinD1 protein level and β-catenin nuclear accumulation at day 7. The matrix mineralization was also augmented after 14 days incubation of GBE. And the 75mg/L was the most effective concentration. Importantly, the positive effects of GBE above were abolished by DKK1, a blocker of the Wnt/β-catenin receptor. Conclusion: GBE has a significantly positive effect on the osteogenic differentiation of the MG63 and the 75mg/L was the most effective concentration, in which Wnt/β-catenin signaling pathway may play an important role.

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