TLR4,NOD2和NLRP3在牙髓炎中的表达

doi:10.13701/j.cnki.kqyxyj.2017.05.008

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (5): 496-499.DOI: 10.13701/j.cnki.kqyxyj.2017.05.008

TLR4,NOD2和NLRP3在牙髓炎中的表达

何琼琼¹,杨颖^2#,黄声富^1*,樊明文^1*

(1. 武汉大学口腔医学院牙体牙髓科湖北武汉 430079;
2. 武汉科技大学附属天佑医院口腔科湖北武汉 430070)

收稿日期:2017-01-13 出版日期:2017-05-20 发布日期:2017-05-26
通讯作者: 黄声富,电话:027- 87647443
#为共同第一作者
作者简介:何琼琼(1990~ ),女,湖南邵阳人,博士在读,主要从事龋病和牙髓病研究。
杨颖(1970~ ),女,湖北武汉人,学士,主治医师,主要从事牙体牙髓的临床治疗工作。
基金资助:
国家自然科学基金项目(编号:81271190)

Expression of TLR4, NOD2 and NLRP3 in Pulpitis.

HE Qiong-qiong¹, YANG Ying^2#, HUANG Sheng-Fu^1*, FAN Ming-wen^1*.

1. School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China; 2. Tianyou hospital affiliated to wuhan university of science ＆ technology, Wuhan 430070, China.

Received:2017-01-13 Online:2017-05-20 Published:2017-05-26

摘要/Abstract

摘要： 目的:分析牙髓炎中TLR4、NOD2和NLRP3的表达特征。方法:收集健康、龋病及牙髓炎的患牙共47颗分为3个组,采用免疫组织化学染色和单色免疫荧光染色方法检测每组牙髓组织中TLR4、NOD2、NLRP3的表达。结果:健康组中TLR4、NOD2和NLRP3仅在成牙本质细胞中少量表达。TLR4、NOD2和NLRP3在牙髓炎组及龋病组表达均明显高于正常组,大量表达于排列紊乱的成牙本质细胞和多种炎症细胞中,而在炎性病灶周边的牙髓成纤维细胞中三者也有表达。结论:本研究表明TLR4、NOD2和NLRP3在炎性牙髓组织中表达同时增高,提示三者均与牙髓炎的进展相关。

关键词: TLR4, NOD2, NLRP3, 牙髓炎

Abstract: Objective: To investigate the expression level of TLR4, NOD2 and NLRP3 in inflamed human dental pulp. Methods: Forty-seven human third molars were divided into three groups: healthy group, carious group, and pulpitis group. The expressions of TLR4, NOD2 and NLRP3 in each group were investigated by immunohistochemistry and immunofluorescence staining. Results: In healthy group, the expressions of TLR4, NOD2, and NLRP3 were only observed in odontoblastic layer. The expression of TLR4, NOD2, and NLRP3 were higher in the caries and pulpitis groups compared with the healthy group. In inflamed dental pulp, TLR4, NOD2, and NLRP3 were detected in odontoblasts, inflammatory cells, and dental pulp fibroblasts. Conclusion: The expressions of TLR4, NOD2, and NLRP3 were simultaneously increased in pulpitis. TLR4, NOD2, and NLRP3 might associate with the progress of pulpitis.

中图分类号:

R781.3

何琼琼,杨颖,黄声富,樊明文. TLR4,NOD2和NLRP3在牙髓炎中的表达[J]. 口腔医学研究, 2017, 33(5): 496-499.

HE Qiong-qiong, YANG Ying, HUANG Sheng-Fu, FAN Ming-wen.. Expression of TLR4, NOD2 and NLRP3 in Pulpitis.[J]. Journal of Oral Science Research, 2017, 33(5): 496-499.

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TLR4,NOD2和NLRP3在牙髓炎中的表达

Expression of TLR4, NOD2 and NLRP3 in Pulpitis.

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