自体新鲜脱矿牙齿移植物在不同部位成骨效果的动物实验

doi:10.13701/j.cnki.kqyxyj.2021.12.018

口腔医学研究 ›› 2021, Vol. 37 ›› Issue (12): 1145-1149.DOI: 10.13701/j.cnki.kqyxyj.2021.12.018

自体新鲜脱矿牙齿移植物在不同部位成骨效果的动物实验

肖健平, 李丽丽, 李厚轩^*, 谭葆春

南京大学医学院附属口腔医院,南京市口腔医院牙周病科江苏南京 210008

收稿日期:2021-02-22 发布日期:2021-12-17
通讯作者: *李厚轩,E-mail: lihouxuan3435_0@163.com
作者简介:肖健平(1973~ ),女,江苏南京人,硕士,副主任医师,研究方向:牙周再生治疗。
基金资助:
江苏省医学创新团队项目(编号:CXTDB2017014) 南京市医学科技发展项目(编号:YKK17139) 南京市口腔疾病临床医学研究中心项目(编号:2019060009)

Animal Study on Osteogenic Effect of Autogenous Fresh Demineralized Tooth Graft in Different Types of Bone Defect

XIAO Jianping, LI Lili, LI Houxuan^*, TAN Baochun

Department of Periodontology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing 210008, China

Received:2021-02-22 Published:2021-12-17

摘要/Abstract

摘要： 目的:研究自体新鲜脱矿牙齿移植物(autogenous fresh demineralized tooth graft,Auto-FDT)应用于位点保存和牙周再生的可行性。方法:拔除2只beagle犬的上下第三切牙,将8个拔牙窝随机分为两组:对照组(4个位点),拔牙后自然愈合,不植入骨粉;实验组(4个位点),拔牙后牙槽窝中植入Auto-FDT及膜。在下颌前磨牙根分叉构建骨缺损模型(10个位点),随机分为两组:对照组(4个位点),只建立骨缺损,不充填骨粉;实验组(6个位点),植入Auto-FDT及膜。12周后安乐处死实验动物,取颌骨标本。通过CBCT检查及病理切片观察成骨效果。结果:CBCT图像显示拔牙位点实验组的平均灰度值显著高于对照组,根分叉病变实验组的平均灰度值显著高于对照组。病理切片可见Auto-FDT能够很好地维持再生空间,并且在颗粒周围观察到活跃的新骨形成。部分的Auto-FDT已经被替代。结论:Auto-FDT有望成为一种安全有效的骨替代材料用于牙周再生治疗。

关键词: 自体新鲜脱矿牙齿移植物, 自体移植物, 牙周再生, 位点保存, 根分叉病变, 骨缺损

Abstract: Objective: To evaluate the practicability of autogenous fresh demineralized tooth graft (Auto-FDT) in preservation of extraction sites and periodontal regeneration. Methods: The upper and lower third incisors of two beagle dogs were extracted, and then the eight extraction sockets were randomly divided into two groups: control group (4 sites), natural healing after tooth extraction, no material implantation; experimental group (4 sites), the Auto-FDT was put into extraction sockets after tooth extraction with barrier membrane covered. Meanwhile, ten furcation bone defects were established at the mandibular premolars. And the defects were randomly divided into two groups: the control group (4 sites), natural healing withought bone graft; the experimental group, implanted the Auto-FDT and membrane. After 12 weeks, the animals were euthanized and the jaw specimens were taken. The effect of bone regeneration was evaluated through CBCT and histological analysis. Results: CBCT images showed that the average gray value of the experimental group of extraction sites was significantly higher than that of the control group, and the average gray value of the experimental group of furcation lesions was significantly higher than that of the control group. Pathological sections showed that grafting of the Auto-FDT maintained the regeneration space well, and active new bone formation was observed around the granules. Part of the Auto-FDT particles were replaced. Conclusion: Autogenous fresh demineralized tooth graft is a promising bone substitute material for periodontal regeneration.

Key words: autogenous fresh demineralized tooth graft, autograft, periodontal regeneration, site preservation, furcation lesion, bone defect

肖健平, 李丽丽, 李厚轩, 谭葆春. 自体新鲜脱矿牙齿移植物在不同部位成骨效果的动物实验[J]. 口腔医学研究, 2021, 37(12): 1145-1149.

XIAO Jianping, LI Lili, LI Houxuan, TAN Baochun. Animal Study on Osteogenic Effect of Autogenous Fresh Demineralized Tooth Graft in Different Types of Bone Defect[J]. Journal of Oral Science Research, 2021, 37(12): 1145-1149.

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自体新鲜脱矿牙齿移植物在不同部位成骨效果的动物实验

Animal Study on Osteogenic Effect of Autogenous Fresh Demineralized Tooth Graft in Different Types of Bone Defect

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