口腔医学研究

口腔医学研究 ›› 2022, Vol. 38 ›› Issue (2): 144-149.DOI: 10.13701/j.cnki.kqyxyj.2022.02.011

• 口腔种植学研究 • 上一篇    下一篇

富血小板纤维蛋白促进牙龈组织修复和再生的研究

王煜慧, 吕慧欣, 张明锐, 王莹莹, 任思聪, 郑世康, 林欣萍, 周延民*   

  1. 吉林大学口腔医学院种植中心 吉林 长春 130021
  • 收稿日期:2021-08-03 出版日期:2022-02-28 发布日期:2022-02-23
  • 通讯作者: *周延民,E-mail:zhouym@jlu.edu.cn
  • 作者简介:王煜慧(1993~),女,硕士,山东潍坊人,研究方向:口腔种植。
  • 基金资助:
    吉林省科技厅项目(编号:20180623051TC)

Study on Platelet-Rich Fibrin Promoting Gingival Tissue Repair and Regeneration

WANG Yuhui, LV Huixin, ZHANG Mingrui, WANG Yingying, REN Sicong, ZHENG Shikang, LIN Xinping, ZHOU Yanmin*   

  1. Department of Oral Implantology, School of Stomatology, Jilin University, Changchun 130021, China
  • Received:2021-08-03 Online:2022-02-28 Published:2022-02-23

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摘要： 目的: 观察富血小板纤维蛋白(platelet-rich fibrin,PRF)在牙龈缺损修复中对原代细胞生物学行为的影响及组织再生作用。方法: 制备PRF膜,扫描电子显微镜(scanning electron microscope,SEM)和组织病理学观察其超微结构;体外实验采用CCK-8法、Transwell实验检测细胞的增殖、迁移能力;实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction,qRT-PCR)检测不同时间点软组织愈合标志物基因COL-I、TGF-β的表达水平。体内实验采用HE染色、免疫组化观察不同时间点组织愈合情况,qRT-PCR检测炎症因子IL-6、TNF-α和TGF-β的表达。结果: 体外实验表明,PRF能够促进人牙龈成纤维细胞(human gingival fibroblast,HGF)的增殖、创伤后的愈合和迁移(P<0.05);培养7 d时PRF组的COL-I、TGF-β表达高于DMEM组(P<0.05);体内实验表明PRF组愈合效果优于Control组;组织学观察,PRF组炎症细胞浸润较Control组少,PRF组血管新生较Control组多;术后7 d, Control组IL-6、TNF-α表达较PRF组更高,Control组TGF-β表达较PRF组低(P<0.05);VEGF免疫组化结果显示,PRF组的阳性细胞表达早于Control组。结论: PRF能促进牙龈中软组织愈合因子COL-I,TGF-β及生长因子VEGF的表达,减小局部炎症反应,加快组织的愈合。

关键词: 富血小板纤维蛋白, 人牙龈成纤维细胞, 细胞增殖, 细胞运动迁移, 创伤愈合

Abstract: Objective: To observe the effect of platelet-rich fibrin on the biological behavior of primary cells and tissue regeneration in the repair of gingival defects. Methods: PRF membrane was prepared. Scanning electron microscope and histopathology were used to observe the ultrastructure. CCK-8 and transwell test was used to detect cell proliferation and migration ability. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of soft tissue healing marker genes COL-I and TGF-β at different time points. HE staining and immunohistochemistry were used to observe tissue healing at different time points, and qRT-PCR was used to detect the expression of inflammatory factors IL-6, TNF-α, and TGF-β. Results: PRF could promote the proliferation, healing, and migration of human gingival fibroblast after trauma (P<0.05). The expressions of COL-I and TGF-β in the PRF group were higher than those in the DMEM group on day 7 (P< 0.05). The healing effect of PRF group was better than that of the control group. Histological observations showed that the PRF group had less inflammatory cell infiltration than the control group, and the PRF group had more angiogenesis than the control group. On day 7 after operation, the expression of IL-6 and TNF-α in the control group was higher than that in the PRF group, and the expression of TGF-β in the control group was lower than that of the PRF group (P<0.05). VEGF immunohistochemical results showed that the expression of positive cells in the PRF group was earlier than that in the control group. Conclusion: PRF can promote the expression of COL-I, TGF-β, and VEGF in the gums, reduce the local inflammation, and accelerate tissue healing.

Key words: platelet-rich fibrin, human gingival fibroblast, cell proliferation, cell movement and migration, wound healing