咬合干扰对小鼠焦虑情绪影响的性别差异

doi:10.13701/j.cnki.kqyxyj.2022.02.019

口腔医学研究 ›› 2022, Vol. 38 ›› Issue (2): 191-196.DOI: 10.13701/j.cnki.kqyxyj.2022.02.019

• 口腔颞下颌关节与牙合学研究 • 上一篇下一篇

咬合干扰对小鼠焦虑情绪影响的性别差异

李娟¹, 徐明¹, 张琪^2*, 刘伟才^1*

1.同济大学口腔医学院,同济大学附属口腔医院修复教研室,上海牙组织修复与再生工程技术研究中心上海 200072;
2.上海市精神卫生中心,上海市重性精神病重点实验室上海 200030

收稿日期:2021-08-18 出版日期:2022-02-28 发布日期:2022-02-23
通讯作者: *张琪,E-mail:ion_zhangqi@126.com;刘伟才,E-mail:vogi@163.com
作者简介:李娟(1995~ ),女,云南腾冲人,硕士,研究方向:咬合与情绪异常。
基金资助:
上海市自然科学基金面上项目(编号:20ZR1463000);上海市自然科学基金面上项目(编号:18ZR1432500)

Gender Differences of Anxiety Induced by Occlusal Interference in Mice

LI Juan¹, XU Ming¹, ZHANG Qi^2*, LIU Weicai^1*

1. Department of Prosthodontics, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, China;
2. Shanghai Key Laboratory for Psychotic Disorders, Shanghai Mental Health Center, Shanghai 200030, China

Received:2021-08-18 Online:2022-02-28 Published:2022-02-23

摘要/Abstract

摘要： 目的: 建立前牙咬合干扰动物模型,观察比较雌雄小鼠的焦虑样行为、情绪调控相关脑区的活化以及血清皮质酮水平。方法: 8周龄C57BL/6小鼠随机分为雄性咬合干扰组、雄性对照组、雌性咬合干扰组以及雌性对照组,造模后第7、14天利用旷场、高架十字迷宫以及黑白箱检测小鼠焦虑情绪变化;通过c-fos染色观察情绪相关脑区神经元的激活水平,血清皮质酮浓度评价机体应激水平。结果: 造模后第7、14天雌雄咬合干扰组小鼠的焦虑样行为增加(P<0.05),但两组间比较差异无统计学意义;咬合干扰后即刻雌雄小鼠前额叶皮层、岛叶皮层、伏隔核、中央杏仁核、基底外侧杏仁核以及丘脑旁下核等脑区神经元激活增加(P<0.05);造模后第14天雌性咬合干扰组小鼠血清皮质酮水平显著增加(P<0.05),而雄性咬合干扰组较对照组比较差异无统计学意义。结论: 前牙咬合干扰短期内可导致雌雄小鼠焦虑样行为增加、情绪相关脑区神经元激活,同时可以导致雌性小鼠机体应激水平增加。

关键词: 咬合干扰, 焦虑, 性别差异, 应激

Abstract: Objective: To observe and compare the anxiety-like behavior of male and female mice, the neural activation in brain regions related to emotional regulation, and corticosterone levels in serum. Methods: 8-week-old C57BL/6 mice were randomly divided into male occlusal interference group, male control group, female occlusal interference group, and female control group. On day 7 and day 14, the anxiety of mice was detected by open field test, elevated plus maze, and light-dark box test, the activation of neurons in emotion-related brain areas was observed by immunohistochemical staining, and the serum corticosterone levels was detected to evaluate the body's stress level. Results: Behavioral test results showed that the anxiety-like behavior of mice in both male and female occlusal interference group increased significantly (P<0.05), but there was no significant statistical difference between these two groups. Immunohistochemical staining results showed that the activation level of medial prefrontal cortex, insular cortex, nucleus accumbens, central amygdala, basolateral amygdala, and parasubthalamic nucleus of male and female mice were increased significantly after occlusal interference (P<0.05). Serum corticosterone results showed that serum corticosterone levels of female occlusal interference group increased significantly (P<0.05), while there was no significant statistical difference in male occlusal interference group on the 14th day after modeling (P>0.05). Conclusion: The experimental occlusal interference of anterior teeth can lead to an increase of anxiety-like behaviors and a significant increase of neuronal activation in emotion-related brain regions in male and female mice in a short period of time, and lead to increased stress levels in female mice.

Key words: occlusal interference, anxiety, gender difference, stress

李娟, 徐明, 张琪, 刘伟才. 咬合干扰对小鼠焦虑情绪影响的性别差异[J]. 口腔医学研究, 2022, 38(2): 191-196.

LI Juan, XU Ming, ZHANG Qi, LIU Weicai. Gender Differences of Anxiety Induced by Occlusal Interference in Mice[J]. Journal of Oral Science Research, 2022, 38(2): 191-196.

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咬合干扰对小鼠焦虑情绪影响的性别差异

Gender Differences of Anxiety Induced by Occlusal Interference in Mice

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