消退素E1对牙髓炎中性粒细胞凋亡的影响

doi:10.13701/j.cnki.kqyxyj.2022.08.009

口腔医学研究 ›› 2022, Vol. 38 ›› Issue (8): 742-746.DOI: 10.13701/j.cnki.kqyxyj.2022.08.009

消退素E1对牙髓炎中性粒细胞凋亡的影响

潘亮亮^1,2, 张旗^1,2*

1.同济大学口腔医学院·同济大学口腔医院牙体牙髓科上海 200072;
2.上海牙组织修复与再生工程技术研究中心上海 200072

收稿日期:2022-04-18 出版日期:2022-08-28 发布日期:2022-08-24
通讯作者: *张旗,E-mail:qizhang@tongji.edu.cn
作者简介:潘亮亮(1996~ ),男,浙江温州人,硕士在读,研究方向:牙髓炎活髓保存治疗。
基金资助:
国家自然科学基金(编号:81870760、82170945)

Effect of Resolvin E1 on Neutrophil Apoptosis in Pulpitis

PAN Liangliang^1,2, ZHANG Qi^1,2*

1. Department of Endodontics, School and Hospital of Stomatology, Tongji University, Shanghai 200072, China;
2. Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, China

Received:2022-04-18 Online:2022-08-28 Published:2022-08-24

摘要/Abstract

摘要： 目的: 探讨消退素E1(resolvin E1,RvE1)影响牙髓炎中性粒细胞凋亡及机制。方法: 建立大鼠牙髓炎模型,RvE1盖髓后1、3、7 d收样。苏木素-伊红(hematoxylin-eosin,HE)染色检测炎症浸润情况,免疫组织化学检测炎症因子表达;免疫荧光CD11b/Tunel共染检测中性粒细胞凋亡水平。分离培养人中性粒细胞,RvE1预处理后加入热灭活变形链球菌(Streptococcus mutans,S.mutan)或脂多糖,6、12、24 h收样。Annexin V/PI检测细胞凋亡水平,qPCR检测凋亡调控因子mRNA表达水平。结果: 大鼠牙髓炎模型中,RvE1处理减少了牙髓炎症浸润与TNF-α和IL-1β水平,提高中性粒细胞凋亡水平。体外RvE1提高了S.mutan刺激后中性粒细胞凋亡水平。qPCR结果显示促生存基因Akt,Mcl-1表达降低,促凋亡基因Bax表达升高。结论: RvE1调控Akt、Mcl-1、Bax表达,增强中性粒细胞细菌刺激后凋亡,促进牙髓炎症消退。

关键词: 中性粒细胞, 凋亡, 炎症消退, 牙髓炎

Abstract: Objective: To investigate the effect and mechanism of resolvin E1 (RvE1) on apoptosis of neutrophils in pulpitis. Methods: SD rats were used to establish the pulpitis model and RvE1 was used for pulp capping. Samples were collected 1, 3, and 7 days after surgery. HE staining was performed to detect inflammatory infiltration, and the expression of inflammatory factors were detected by immunohistochemistry. CD11b and Tunel co-staining were used to detect the neutrophil apoptosis rate. Human neutrophils were isolated and cultured with heat-inactived Streptococcus mutans (S.mutan) or lipopolysaccharide after pretreated with RvE1. Samples were collected 6, 12, and 24 hours after stimulation. Annexin V/PI was used to detect the apoptosis rate and qPCR was used to detect mRNA expression of apoptosis related factors. Results: In vivo, RvE1 treatment significantly reduced the pulpitis infiltration and expressions of TNF-α and IL-1β, and increased the neutrophil apoptosis rate. In vitro, RvE1 treatment increased the apoptosis rate of neutrophil after S.mutan stimulation. QPCR results showed that the expression of pro-survival gene Akt and Mcl-1 decreased, and the expression of pro-apoptotic gene Bax increased. Conclusion: RvE1 regulates the expression of Akt, Mcl-1, and Bax, enhances the apoptosis of neutrophils after bacteria stimulation, and promotes the regression of pulpitis.

Key words: neutrophil, apoptosis, resolution, pulplitis

潘亮亮, 张旗. 消退素E1对牙髓炎中性粒细胞凋亡的影响[J]. 口腔医学研究, 2022, 38(8): 742-746.

PAN Liangliang, ZHANG Qi. Effect of Resolvin E1 on Neutrophil Apoptosis in Pulpitis[J]. Journal of Oral Science Research, 2022, 38(8): 742-746.

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消退素E1对牙髓炎中性粒细胞凋亡的影响

Effect of Resolvin E1 on Neutrophil Apoptosis in Pulpitis

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