Bcl-2和p53蛋白在牙龈增生中的表达研究

doi:10.13701/j.cnki.kqyxyj.2021.04.006

口腔医学研究 ›› 2021, Vol. 37 ›› Issue (4): 300-303.DOI: 10.13701/j.cnki.kqyxyj.2021.04.006

Bcl-2和p53蛋白在牙龈增生中的表达研究

张睿^1*, 刘娇艳², 李德纯², 张明珠¹, 彭艺¹, 税艳青¹

1.昆明医科大学附属口腔医院牙周病科云南昆明 650100;
2.昆明医科大学口腔医学院云南昆明 650500

收稿日期:2020-09-24 发布日期:2021-04-15
通讯作者: *张睿,E-mail:274674103@qq.com
作者简介:张睿(1988~ ),女,云南人,医师,硕士,主要从事牙周病学相关基础研究和临床工作。
基金资助:
昆明医科大学大学生创新性实验计划省级项目(329)

Expressions of Bcl-2 and p53 Proteins in Gingival Hyperplasia

ZHANG Rui^1*, LIU Jiaoyan², LI Dechun², ZHANG Mingzhu¹, PENG Yi¹, SHUI Yanqing¹

1. Department of Periodontics, The Affiliated Stomatology Hospital of Kunming Medical University, Kunming 650100, China;
2. School of Stomatology, Kunming Medical University, Kunming 650500, China

Received:2020-09-24 Published:2021-04-15

摘要/Abstract

摘要： 目的: 研究Bcl-2(B-cell leukemia-2)蛋白和p53蛋白在牙龈增生中的表达,探讨其与增生牙龈中抗凋亡作用机制的相关性。方法: 选择符合临床诊断的11例炎症性牙龈增生患者作为实验组,正常牙龈组织11例作为对照组;通过免疫组织化学法探究增生牙龈组织和正常牙龈组织中Bcl-2蛋白和p53蛋白的表达情况。结果: 增生牙龈组织中Bcl-2蛋白和p53蛋白的阳性表达均明显高于正常牙龈组织(P<0.05)。结论: Bcl-2蛋白和p53蛋白在牙龈增生中发挥重要作用,抗凋亡抑制作用参与了牙龈增生的发病过程。

关键词: 牙龈增生, 抗凋亡作用, Bcl-2蛋白, p53蛋白

Abstract: Objective: To study the expressions of Bcl-2 protein and p53 protein in gingival hyperplasia, and to explore their correlation with the anti-apoptotic mechanism of gingival hyperplasia. Methods: 11 cases of gingival hyperplasia caused by inflammation in accordance with the clinical diagnosis were selected as the experimental group and 11 cases of normal gingival tissue as the control group. The expressions of Bcl-2 and p53 proteins in the hyperplastic and normal gingival tissue were investigated by immunohistochemistry. Results: The positive expressions of Bcl-2 protein and p53 protein in hyperplastic gingival tissue were both significantly higher than those in normal gingival tissue (P<0.05). Conclusion: Bcl-2 protein and p53 protein play an important role in gingival hyperplasia, and the anti-apoptosis may involve in the pathogenesis of gingival hyperplasia.

Key words: gingival hyperplasia, anti-apoptosis, Bcl-2 protein, p53 protein

张睿, 刘娇艳, 李德纯, 张明珠, 彭艺, 税艳青. Bcl-2和p53蛋白在牙龈增生中的表达研究[J]. 口腔医学研究, 2021, 37(4): 300-303.

ZHANG Rui, LIU Jiaoyan, LI Dechun, ZHANG Mingzhu, PENG Yi, SHUI Yanqing. Expressions of Bcl-2 and p53 Proteins in Gingival Hyperplasia[J]. Journal of Oral Science Research, 2021, 37(4): 300-303.

参考文献

[1] 刘培红,马肃.环孢素诱导牙龈上皮细胞增生的机制[J].国际口腔医学杂志,2009,36(1):81-83.
[2] 陈诗书,汤雪明.医学细胞与分子生物学[M].第2版.北京: 科学出版社, 2003: 532-533.
[3] 黄雅玲,李升,杨明华.药物性牙龈增生发病机制的研究进展[J].国际口腔医学杂志, 2008,35(4):414-417.
[4] De Angelis PM, Stokke T, Thorstensen L, et al. Apoptosis and expression of Bax, Bcl-x, and Bcl-2 apoptotic regulatory proteins in colorectal carcinomas, and association with p53 genotype/phenotype [J]. Mol Pathol, 1998, 51(5):254-261.
[5] 焦晓菊, 周佳, 徐烁, 等. 药物性牙龈增生发病机制的研究进展[J].口腔医学研究,2018,34(4):350-352.
[6] 王晓乐, 贾荣. 口腔鳞状细胞癌患者年龄分布与肿瘤分子标记物的关系研究[J].口腔医学研究,2019,35(3):238-241.
[7] Michael Newman, Henry Takei, Perry Klokkevold, et al. Newman and Carranza's clinical periodontology [M]. 13th ed. Philadelphia: Elsevier, 2019.
[8] 朱玉侠, 赵明星, 姜登鸽, 等. 抑癌基因p53、凋亡抑制基因Bcl-2、促凋亡基因Bax在胃癌及癌前病变中的表达[J].胃肠病学和肝病学杂志, 2016,25(9):1040-1043.
[9] Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease [J]. Exp Gerontol, 2004, 39(8):1125-1135.
[10] 方首镕. Bcl-2、MIF蛋白在老年牙龈癌组织中的表达与临床意义[J].上海口腔医学, 2020, 29(1):105-108.
[11] 张强, 刘继刚, 刘江, 等. Bcl-2及DPC4在牙龈癌中的表达及相关性研究[J].牙体牙髓牙周病学杂志, 2015, 25(10):584-586+583.
[12] 王美娟, 孙卫斌, 刘卫红, 等. p53蛋白和Bcl-2蛋白在环孢菌素和硝苯地平龈增生中的表达[J].口腔医学, 2004,24(5):300-302.
[13] 张明珠, 张蕾, 朱红, 等. bcl-2蛋白在血管性和纤维性牙龈瘤中的表达和意义[J].牙体牙髓牙周病学杂志, 2013,23(8):503-506.
[14] 史珂, 张明珠, 刘彦, 等. 增生性龈炎和牙龈瘤中bcl-2、bax基因表达的实验[J].昆明医科大学学报, 2019,40(10):11-14.
[15] 赵卫红,寿好长,闽福岭. 细胞凋亡[M].郑州: 河南医科大学出版社, 1997: 19-21.
[16] Vikhanskaya F, Lee MK, Mazzoletti M, et al. Cancer-derived p53 mutants suppress p53-target gene expression-potential mechanism for gain of function of mutant p53 [J]. Nucleic Acids Res, 2007, 35(6):2093-2104.
[17] Leyva-Huerta ER, Ledesma-Montes C, Rojo-Botello RE, et al. p53 and bcl-2 immunoexpression in patients with oral lichen planus and oral squamous cell carcinoma [J]. Med Oral Patol Oral Cir Bucal, 2012,17(5):e745-e750.
[18] Saito K, Mori S, Tanda N, et al. Immunolocalizaiton of c-Myc and bcl-2 proto-oncogene products in gingival hyperplasia induced by nifedipine and phenytoin [J]. J Periodontol, 2000, 71(1):44-49.
[19] Liu J, Zeng J, Wang X, et al. p53 mediates lipopolysaccharide-induced inflammation in human gingival fibroblasts [J]. J Periodontol, 2018, 89(9):1142-1151.

Bcl-2和p53蛋白在牙龈增生中的表达研究

Expressions of Bcl-2 and p53 Proteins in Gingival Hyperplasia

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