口腔医学研究

口腔医学研究 ›› 2022, Vol. 38 ›› Issue (10): 986-990.DOI: 10.13701/j.cnki.kqyxyj.2022.10.018

• 其他研究 • 上一篇    下一篇

吸烟与非吸烟者口腔微生物多样性形成机制对比分析

康吉哲1, WANG Wei2, 叶俊杰1, 杨阳3, 董琛1*   

  1. 1.山东体育学院健康服务与管理教研室 山东 济南 250102;
    2.德国慕尼黑大学人类科学中心 德国 慕尼黑 280539;
    3.山东省立医院 山东 济南 250021
  • 收稿日期:2022-03-17 出版日期:2022-10-28 发布日期:2022-10-20
  • 通讯作者: *董琛,E-mail: dongchen@sdpei.edu.cn
  • 作者简介:康吉哲(1999~ ),男,山东滨州人,硕士在读,研究方向:口腔微生物学。
  • 基金资助:
    国家社科基金重点项目(编号:18ATY002);山东省青创人才引育团队"健康服务与管理研究"项目(编号:72);山东体育学院高层次人才引进科研启动经费

Comparative Analysis on Formation Mechanism of Oral Microbial Diversity Between Smokers and Non-smokers

KANG Jizhe1, WANG Wei2, YE Junjie1, YANG Yang3, DONG Chen1*   

  1. 1. Health Service and Management Research Office, Shandong Sport University, Jinan 250102, China;
    2. Human Science Center, Ludwig-Maximilians-Universität, Munich 280539, Germany;
    3. Shandong Provincial Hospital, Jinan 250021, China
  • Received:2022-03-17 Online:2022-10-28 Published:2022-10-20

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摘要： 目的: 探究烟草对口腔微生物多样性形成与演替影响机制。方法: 追踪调查了吸烟和非吸烟者各20例4个月口腔的5个常见菌属——链球菌属、普氏菌属、嗜血杆菌属、罗氏菌属和韦荣氏菌属的比生长速率、胞内脂类物质含量及种群数量变化。结果: 非吸烟者口腔微生物胞内的脂类物质含量显著增高,而比生长速率却有不同的变化,而各菌群动态呈现出明显的异步波动趋势。非吸烟者口腔微生物多样性形成与维持包括两种基本动力学机制:一是底物分解产物对种群的负反馈调节,二是微生物对口腔环境发生的时滞响应。而吸烟者恰恰相反,口腔菌群多样性降低且有紊乱的趋势。结论: 吸烟对口腔菌群多样性产生负面影响,不易形成稳定种群多样性;非吸烟者口腔微生物群落中的各菌种数量呈现异步趋同波动,从而大大减弱菌群内部的种间竞争,在口腔中形成与维持较高的物种多样性。

关键词: 微生物多样性, 反馈调节, 时滞响应, 元胞自动机模型, 计算机仿真

Abstract: Objective: To explore the influence mechanism of tobacco on the formation and succession of oral microbial diversity. Methods: The specific growth rate, lipid content, and population number of Streptococcus, Prevotella, Haemophilus, Rothia, and Veillonella were investigated in the oral cavity of 20 smokers and 20 non-smokers for 4 months. Results: The intracellular lipid content of non-smoker oral microorganisms increased significantly, but the specific growth rate varied. Formation and maintain non-smoker oral microbial diversity included two basic dynamic mechanism, one was the substrate decomposition product negative feedback regulation of population, and the second was the delay response of microorganisms in oral environment. On the contrary, in smokers, the diversity of oral flora decreased and had a trend of disorder. Conclusion: Smoking has a negative effect on the diversity of oral microflora, which is difficult to form a stable population diversity. The number of each species in non-smokers oral microbial community showed asynchronous convergence fluctuations, which greatly weakened the interspecific competition within the oral microbiome and formed and maintained a higher species diversity.

Key words: microbial diversity, feedback regulation, time-delay response, cellular automata model, computer simulation