SMURF2/β-catenin轴促进张力条件下人牙周膜细胞成骨分化

doi:10.13701/j.cnki.kqyxyj.2023.07.010

口腔医学研究 ›› 2023, Vol. 39 ›› Issue (7): 619-624.DOI: 10.13701/j.cnki.kqyxyj.2023.07.010

SMURF2/β-catenin轴促进张力条件下人牙周膜细胞成骨分化

张小岑¹, 常茂琳^1,2#, 邹浩¹, 刘小瑜¹, 韩光丽^1,2*,

1.口颌系统重建与再生全国重点实验室,口腔生物医学教育部重点实验室,口腔医学湖北省重点实验室,武汉大学口腔医(学)院湖北武汉 430079;
2.武汉大学口腔医院正畸二科湖北武汉 430079

收稿日期:2023-03-06 发布日期:2023-07-25
通讯作者: *韩光丽,E-mail: guanglihan@whu.edu.cn
作者简介:张小岑(1994~ ),男,湖北咸宁人,硕士在读,主要从事口腔正畸学研究。常茂琳(1990~ ),男,安徽毫州人,博士,主治医师,主要从事口腔正畸学研究。#为共同第一作者
基金资助:
国家自然科学基金(编号:81771111)

SMURF2/β-catenin Axis Promotes Osteogenic Differentiation of Human Periodontal Ligament Cells under Cyclic Stretch

ZHANG Xiaocen¹, CHANG Maolin^1,2#, ZOU Hao¹, LIU Xiaoyu¹, HAN Guangli^1,2*

1. State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University Wuhan 430079, China;
2. Department of Orthodontics Division 2, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China

Received:2023-03-06 Published:2023-07-25

摘要/Abstract

摘要： 目的:探究Smad泛素化调节因子2(SMURF2)在循环牵张力诱导人牙周膜细胞(hPDLCs)成骨分化中的作用和机制。方法:对hPDLCs施加循环牵张力刺激后使用qRT-PCR检测SMURF2的表达和成骨相关基因的表达,使用western blotting检测SMURF2和经典WNT信号相关分子的表达,通过ALP染色检测hPDLCs的ALP活性,并结合成骨相关基因的表达评估hPDLCs的成骨分化。通过使用小干扰RNA沉默hPDLCs中的SMURF2基因探究 SMURF2对牵张力诱导的hPDLCs成骨分化和经典WNT信号的影响。结果:循环牵张力刺激hPDLCs后SMURF2、ALP、OPN、OSX、COL-1和β-catenin表达升高,ALP活性也增强。沉默SMURF2基因后,hPDLCs的ALP活性减弱,ALP、OPN和OSX基因表达水平下降,β-catenin蛋白下降,但GSK-3β蛋白无变化。结论:SMURF2可能通过经典WNT信号通路促进循环牵张力诱导的hPDLCs成骨分化。

关键词: 循环牵张力, 人牙周膜细胞, 成骨分化, Smad泛素化调节因子2

Abstract: Objective: To investigate the effects and mechanisms of Smad ubiquitylation regulatory factor 2(SMURF2) in cyclic stretch-induced osteogenic differentiation of human periodontal ligament cells (hPDLCs). Methods: Cyclic stretch was applied on hPDLCs. The expression of SMURF2 and osteogenic genes were analyzed by qRT-PCR. The expression of SMURF2 and molecules mediating canonical WNT signaling were examined by western blotting. The osteogenic differentiation of hPDLCs was assessed by ALP staining and expression of osteogenic genes. A small interfering RNA was used to knockdown SMURF2 in hPDLCs to investigate effects of SMURF2 on the osteogenic differentiation of hPDLCs and canonical WNT signaling. Results: Expressions of SMURF2, ALP, OPN, OSX, COL-1, and β-catenin were up-regulated in hPDLCs applied cyclic stretch. Activity of ALP was also up-regulated in stretched cells. Depletion of SMURF2 impaired the activity of ALP, down-regulated the expression of ALP,OPN, and OSX genes, and β-catenin protein. However, the protein level of GSK-3β was not changed after SMURF2 depletion. Conclusion: SMURF2 may promote cyclic stretch-induced osteogenic differentiation of hPDLCs through canonical WNT signaling pathway.

Key words: cyclic stretch, human periodontal ligament cells, osteogenic differentiation, smad ubiquitylation regulatory factor 2

张小岑, 常茂琳, 邹浩, 刘小瑜, 韩光丽,. SMURF2/β-catenin轴促进张力条件下人牙周膜细胞成骨分化[J]. 口腔医学研究, 2023, 39(7): 619-624.

ZHANG Xiaocen, CHANG Maolin, ZOU Hao, LIU Xiaoyu, HAN Guangli. SMURF2/β-catenin Axis Promotes Osteogenic Differentiation of Human Periodontal Ligament Cells under Cyclic Stretch[J]. Journal of Oral Science Research, 2023, 39(7): 619-624.

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SMURF2/β-catenin轴促进张力条件下人牙周膜细胞成骨分化

SMURF2/β-catenin Axis Promotes Osteogenic Differentiation of Human Periodontal Ligament Cells under Cyclic Stretch

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