FTA-DNA直接提取法在种植体周围炎/牙周炎检测的效果评价

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (11): 1089-1092.

FTA-DNA直接提取法在种植体周围炎/牙周炎检测的效果评价

吴亚婷,聂红兵^*,王子剑,王佳佳,周建业,郭曼丽

西北民族大学口腔医院甘肃兰州 730030

收稿日期:2015-03-26 出版日期:2015-11-28 发布日期:2016-03-21
通讯作者: 聂红兵,电话:13619365767
作者简介:吴亚婷(1992~),女,宁夏固原人,本科在读,主要从事口腔微生物及口腔医学全科的学习。
基金资助:
2014国家级大学生创新创业训练计划项目(编号:201410742013)

Evaluation of FTA-DNA Direct Extraction Method in the Detection of Peri-implantitis or Periodontitis.

WU Ya-ting, NIE Hong-bing, WANG Zi-jian, WANG Jia-jia, ZHOU Jian-ye, GUO Man-li.

Stomatology Hospital, Northwest University for Nationalities, Lanzhou 730030, China

Received:2015-03-26 Online:2015-11-28 Published:2016-03-21

摘要/Abstract

摘要： 目的:建立并评价FTA-DNA直接提取法在种植体周围炎和牙周炎细菌DNA扩增中的应用效果。方法:选取14例种植体周围炎患者和26例种植体周围炎患者,用传统法、煮沸法和FTA-DNA直接提取法分别提取DNA,同时进行16S-rDNA扩增,凝胶电泳检测,比较3种DNA提取方法的差异。结果:种植体周围炎和牙周炎之间差异无显著性,3种方法提取的DNA在PCR扩增后均有条带,但FTA-DNA直接提取法成功率高于试剂盒法和煮沸法,存在统计学差异;且FTA-DNA直接提取法在提取的步骤、时间、费用上有较高的优势。结论:FTA-DNA直接提取法可快速有效地从种植体周围炎和牙周炎临床标本中提取并保存细菌DNA,有望用于种植体周围炎和牙周炎的研究及临床诊断。

Abstract: Objective: To evaluate the effect of FTA-DNA direct extraction method in the detection of peri-implantitis (PI) and periodontitis. Methods: Fourteen PI patients and 26 periodontitis patients were selected. Traditional method, boiling method, and FTA-DNA direct extraction method were used respectively to extract DNA. 16S-rDNA was amplified and gel electrophoresis was carried to compare the differences among three DNA extraction methods. Results: There was no significant difference between the peri-implantitis and periodontitis. DNA could be extracted by these three methods. FTA-DNA direct extraction significantly increased the success rate, when compared with traditional method and boiling method. Conclusion: Bacterial DNA in PI and periodontitis patients can be efficiently extracted and saved by FTA-DNA direct extraction method, which may be used to study and diagnose peri-implantitis and periodontitis.

中图分类号:

R781.4

吴亚婷,聂红兵,王子剑,王佳佳,周建业,郭曼丽. FTA-DNA直接提取法在种植体周围炎/牙周炎检测的效果评价[J]. 口腔医学研究, 2015, 31(11): 1089-1092.

WU Ya-ting, NIE Hong-bing, WANG Zi-jian, WANG Jia-jia, ZHOU Jian-ye, GUO Man-li.. Evaluation of FTA-DNA Direct Extraction Method in the Detection of Peri-implantitis or Periodontitis.[J]. Journal of Oral Science Research, 2015, 31(11): 1089-1092.

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