口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (11): 1161-1164.DOI: 10.13701/j.cnki.kqyxyj.2017.11.007

• 牙周组织研究 • 上一篇    下一篇

Th17及其相关因子在不同牙周炎症状态龈沟液中的检测

杨栋1, 2, 李红梅2, 刘林花2, 董维理1, 2*   

  1. 1. 武汉大学口腔医学院牙周科 湖北 武汉 430079;
    2. 口腔基础医学省部共建国家重点实验室培育基地和口腔生物医学教育部重点实验室 湖北 武汉 430079
  • 收稿日期:2017-08-09 出版日期:2017-11-20 发布日期:2017-11-29
  • 通讯作者: 董维理,E-mail:zdwlss272@whu.edu.cn
  • 作者简介:杨栋(1986~ ),男,博士,主治医师,主要从事牙周病的病因研究与临床。
  • 基金资助:
    湖北省卫生计生科研基金(编号:WJ2015MB040)

Detection of Th17 and Related Factors in Gingival Crevicular Fluid of Patients with Different Periodontal Inflammatory Status

YANG Dong1,2, LI Hong-mei2, LIU Lin-hua2, DONG Wei-li1,2*   

  1. 1. School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China;
    2. The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China
  • Received:2017-08-09 Online:2017-11-20 Published:2017-11-29

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摘要： 目的:探讨Th17及其相关因子在慢性牙周炎发病中的作用。方法:选择46例牙周科患者分为4组:慢性牙周炎活动型位点组、慢性牙周炎静止型位点组、牙龈炎组和健康对照组,应用酶联免疫法检测各组龈沟液中IL-17、RORC2、IFN-γ和IL-4的含量并进行分析。结果:IL-17和RORC2的表达趋势相似,活动型位点组明显高于其他3组,静止型位点组明显高于健康对照组;健康对照组的IFN-γ明显低于其他3组;活动型位点组IL-4明显低于其他3组;IL-17和RORC2呈正相关,IL-17和IL-4呈负相关;IL-17、RORC2、IFN-γ与临床指标BI、PD和AL呈正相关,IL-4与BI、PD和AL呈负相关。结论:Th17在慢性牙周炎活动型中占优势,随着附着丧失增加,龈沟液中含量升高;IFN-γ可能参与了牙龈炎症和非炎症的状态转化。

关键词: 牙周炎, IL-17, RORC2, IFN-γ, IL-4, 龈沟液

Abstract: Objective: To study the correlation of Th17 and related factors in the gingival crevicular fluid (GCF) of the patients with different periodontal inflammatory status. Methods: Forty-six patients were divided into four groups: periodontitis active site group, non-active site group, gingivitis group, and healthy group. IL-17, RORC2, IFN-γ, and IL-4 in GCF were detected by enzyme-linked immunosorbent assay (ELISA). Results: The expression trend of IL-17 and RORC2 were similar. The levels of IL-17 and RORC2 in active site group were significantly higher than those of other three groups, and the levels of non-active site group were significantly higher than that of healthy group (P<0.05). The expression of IFN-γ in healthy group was significantly lower than those of other three groups (P<0.05). In active periodontal site group, the expression level of IL-4 was significantly lower than those of other three groups (P<0.05). IL-17 level was positively correlated with RORC2, and negatively correlated with IL-4 (P<0.05). The levels of IL-17, RORC2 and IFN-γ were positively correlated with those of BI, PD and AL. The level of IL-4 was negatively correlated with those of BI, PD and AL (P<0.05). Conclusion: The expression of Th17 was strong in active site group, and it increased with the aggravation of attachment loss. IFN-γ might be involved in the transformation of gingival inflammation and non-inflammation status.

Key words: Periodontitis, IL-17, RORC2, IFN-γ, IL-4, GCF

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